Non-Blocking Signature of very large SOAP Messages

Data transfer and staging services are common components in Grid-based, or more generally, in service-oriented applications. Security mechanisms play a central role in such services, especially when they are deployed in sensitive application fields like e-health. The adoption of WS-Security and related standards to SOAP-based transfer services is, however, problematic as a straightforward adoption of SOAP with MTOM introduces considerable inefficiencies in the signature generation process when large data sets are involved. This paper proposes a non-blocking, signature generation approach enabling a stream-like processing with considerable performance enhancements.Comment: 13 pages, 5 figure

Non-Blocking Signature of very large SOAP Messages

Data transfer and staging services are common components in Grid-based, or more generally, in service-oriented applications. Security mechanisms play a central role in such services, especially when they are deployed in sensitive application fields like e-health. The adoption of WS-Security and related standards to SOAP-based transfer services is, however, problematic as a straightforward adoption of SOAP with MTOM introduces considerable inefficiencies in the signature generation process when large data sets are involved. This paper proposes a non-blocking, signature generation approach enabling a stream-like processing with considerable performance enhancements.Comment: 13 pages, 5 figure

Durable resistance to crop pathogens: an epidemiological framework to predict risk under uncertainty.

Increasing the durability of crop resistance to plant pathogens is one of the key goals of virulence management. Despite the recognition of the importance of demographic and environmental stochasticity on the dynamics of an epidemic, their effects on the evolution of the pathogen and durability of resistance has not received attention. We formulated a stochastic epidemiological model, based on the Kramer-Moyal expansion of the Master Equation, to investigate how random fluctuations affect the dynamics of an epidemic and how these effects feed through to the evolution of the pathogen and durability of resistance. We focused on two hypotheses: firstly, a previous deterministic model has suggested that the effect of cropping ratio (the proportion of land area occupied by the resistant crop) on the durability of crop resistance is negligible. Increasing the cropping ratio increases the area of uninfected host, but the resistance is more rapidly broken; these two effects counteract each other. We tested the hypothesis that similar counteracting effects would occur when we take account of demographic stochasticity, but found that the durability does depend on the cropping ratio. Secondly, we tested whether a superimposed external source of stochasticity (for example due to environmental variation or to intermittent fungicide application) interacts with the intrinsic demographic fluctuations and how such interaction affects the durability of resistance. We show that in the pathosystem considered here, in general large stochastic fluctuations in epidemics enhance extinction of the pathogen. This is more likely to occur at large cropping ratios and for particular frequencies of the periodic external perturbation (stochastic resonance). The results suggest possible disease control practises by exploiting the natural sources of stochasticity.GL is funded by the ESPA award “Dynamic Drivers of Disease in Africa Consortium”. The work of FvdB is supported by Rothamsted Research, who receives grant aided assistance from the Biological and Biotechnological Research Council of the United Kingdom. CAG gratefully acknowledges the support of a BBSRC Professorial Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.This is the final version of the article. It first appeared from PLOS via http://dx.doi.org/10.1371/journal.pcbi.100287

An optimized procedure for preparation of conditioned medium from Wharton’s jelly mesenchymal stromal cells isolated from umbilical cord

Cell-free therapy based on conditioned medium derived from mesenchymal stromal cells (MSCs) has gained attention in the field of protective and regenerative medicine. However, the exact composition and properties of MSC-derived conditioned media can vary greatly depending on multiple parameters, which hamper standardization. In this study, we have optimized a procedure for preparation of conditioned medium starting from efficient isolation, propagation and characterization of MSCs from human umbilical cord, using a culture medium supplemented with human platelet lysate as an alternative source to fetal bovine serum. Our procedure successfully maximizes the yield of viable MSCs that maintain canonical key features. Importantly, under these conditions, the compositional profile and biological effects elicited by the conditioned medium preparations derived from these MSC populations do not depend on donor individuality. Moreover, approximately 120 L of conditioned medium could be obtained from a single umbilical cord, which provides a suitable framework to produce industrial amounts of toxic-free conditioned medium with predictable composition

ANATOMICAL VARIATIONS OF THE INTERNAL JUGULAR VEIN: THE ROLE OF ULTRASONOGRAPHY

Purpose: In many places, especially in emergency department, central venous catheter is still inserted using anatomical landmark guidance with a success rate up to 97.6% and complications up to 15%. This study was aimed to determine by the support of ultrasono-graphy (US) the anatomical variations of the internal jugular vein (IJV) in relation with other structures of the neck, such as the common carotid artery (CCA). Material and Methods: 830 patients requiring central vein catheterization (CVC) were in-cluded in the analysis. The position of the IJV in relation to the other structures of the neck was demonstrated by portable ultrasonography. Results: The mean diameter of IJV was 10.3 mm in right and 10.5 mm in left side of neck, in male (p > 0.05) and 9.1 mm in right and 10.5 mm in left side of neck, in female (p > 0.05). The mean distance between IJV and CCA was 1.9 mm in right and 1.7 mm in left side of neck in male, and 2.0 mm in right and 2.2 mm in left side of neck in female. The mean distance of IJV from the skin surface was 9.8 mm in right and 10.0 mm in left side of neck in male, and 12.1 mm in right and 12.5 mm in left side of neck in female. On 25.54 % we observed variations of internal jugular vein site. On 3.97 % we observed a small caliber of internal jugular vein that could complicate the catheterization of the vein. On 1.8 % was diagnosed a thrombus of internal jugular vein, that is considered as an ab-solute contraindication for a CVC. Conclusion: Different patients had anatomical variations that are important and should be knowed, in order to reduce the possibility of severe complication

Gamma-hydroxybutyrate (GHB) for prevention and treatment of alcohol withdrawal

This is the protocol for a review and there is no abstract. The objectives are as follows: To evaluate the efficacy and safety of GHB in prevention and treatment of the AWS, more specifically • to compare the efficacy of GHB with placebo or other drugs; • to identify the most effective GHB dosage and schedules; • to estimate the incidence of side effects; • to carry out a risk-benefit analysis

Geo-hazards of the San Vito peninsula offshore (southwestern Tyrrhenian Sea)

In this paper we present geomorphological hazard mapping of the San Vito Peninsula offshore (Sicilian margin of Tyrrhenian Sea), characterised by a very narrow continental shelf and a very dipping, tectonically active continental slope, no far from a seismogenic belt. The data set consists of morpho-bathymetric models achieved by means of multibeam survey and scattered high resolution seismic profiles. The morpho-bathymetric study highlighted some potential sites of geomorphological hazards, the most representative of which are: (i) two canyons developing from the upper slope off San Vito Cape down to the Erice basin; (ii) a dense network of small incisions across the upper slope; (iii) a very active landslide, developed onland and extending in the continental shelf close to the Scopello village. The geomorphological features that can generate submarine hazard are represented by: unstable sediment packages into canyon heads or along the upper slope; submarine canyons with regressive erosion; coastal landslides

Thoracoscopy in pleural effusion –two techniques: awake single-access video-assisted thoracic surgery versus 2-ports video-assisted thoracic surgery under general anesthesia

Awake single access video-assisted thoracic surgery with local anesthesia improves procedure tolerance, reduces postoperative stay and costs. MATERIALS & METHODS: Local anesthesia was made with lidocaine and ropivacaine. We realize one 20 mm incision for the 'single-access', and two incisions for the '2-trocars technique'. RESULTS: Mortality rate was 0% in both groups. Postoperative stay: 3dd ± 4 versus 4dd ± 5, mean operative time: 39 min versus 37 min (p < 0.05). Chest tube duration: 2dd ± 5 versus 3dd ± 6. COMPLICATIONS: 11/95 versus 10/79. CONCLUSION: Awake technique reduce postoperative hospital stay and chest drainage duration, similar complications and recurrence rate. The authors can say that 'awake single-access VATS' is an optimal diagnostic and therapeutic tool for the management of pleural effusions, but above extends surgical indication to high-risk patients

Where are the horses? With the sheep or cows? Uncertain host location, vector-feeding preferences and the risk of African horse sickness transmission in Great Britain

Understanding the influence of non-susceptible hosts on vector-borne disease transmission is an important epidemiological problem. However, investigation of its impact can be complicated by uncertainty in the location of the hosts. Estimating the risk of transmission of African horse sickness (AHS) in Great Britain (GB), a virus transmitted by Culicoides biting midges, provides an insightful example because: (i) the patterns of risk are expected to be influenced by the presence of non-susceptible vertebrate hosts (cattle and sheep) and (ii) incomplete information on the spatial distribution of horses is available because the GB National Equine Database records owner, rather than horse, locations. Here, we combine land-use data with available horse owner distributions and, using a Bayesian approach, infer a realistic distribution for the location of horses. We estimate the risk of an outbreak of AHS in GB, using the basic reproduction number (R0), and demonstrate that mapping owner addresses as a proxy for horse location significantly underestimates the risk. We clarify the role of non-susceptible vertebrate hosts by showing that the risk of disease in the presence of many hosts (susceptible and non-susceptible) can be ultimately reduced to two fundamental factors: first, the abundance of vectors and how this depends on host density, and, second, the differential feeding preference of vectors among animal species