6 research outputs found
In Vitro Resistance Profile of the Hepatitis C Virus NS3 Protease Inhibitor BI 201335
The in vitro resistance profile of BI 201335 was evaluated through selection and characterization of variants in genotype 1a (GT 1a) and genotype 1b (GT 1b) replicons. NS3 R155K and D168V were the most frequently observed resistant variants. Phenotypic characterization of the mutants revealed shifts in sensitivity specific to BI 201335 that did not alter susceptibility to alpha interferon. In contrast to macrocyclic and covalent protease inhibitors, changes at V36, T54, F43, and Q80 did not confer resistance to BI 201335
Discovery of Potent, Orally Bioavailable Inhibitors of Human Cytomegalovirus
A high-throughput
screen based on a viral replication assay was
used to identify inhibitors of the human cytomegalovirus. Using this
approach, hit compound <b>1</b> was identified as a 4 μM
inhibitor of HCMV that was specific and selective over other herpes
viruses. Time of addition studies indicated compound <b>1</b> exerted its antiviral effect early in the viral life cycle. Mechanism
of action studies also revealed that this series inhibited infection
of MRC-5 and ARPE19 cells by free virus and via direct cell-to-cell
spread from infected to uninfected cells. Preliminary structure–activity
relationships demonstrated that the potency of compound <b>1</b> could be improved to a low nanomolar level, but metabolic stability
was a key optimization parameter for this series. A strategy focused
on minimizing metabolic hydrolysis of the N1-amide led to an alternative
scaffold in this series with improved metabolic stability and good
pharmacokinetic parameters in rat