First Whole Genome Sequence of Anaplasma platys, an Obligate Intracellula Rickettsial Pathogen of Dogs

We have assembled the first genome draft of Anaplasma platys, an obligate intracellular rickettsia, and the only known bacterial pathogen infecting canine platelets. A. platys is a not-yet-cultivated bacterium that causes infectious cyclic canine thrombocytopenia, a potentially fatal disease in dogs. Despite its global distribution and veterinary relevance, no genome sequence has been published so far for this pathogen. Here, we used a strategy based on metagenome assembly to generate a draft of the A. platys genome using the blood of an infected dog. The assembled draft is similar to other Anaplasma genomes in size, gene content, and synteny. Notable differences are the apparent absence of rbfA, a gene encoding a 30S ribosome-binding factor acting as a cold-shock protein, as well as two genes involved in biotin metabolism. We also observed differences associated with expanded gene families, including those encoding outer membrane proteins, a type IV secretion system, ankyrin repeat-containing proteins, and proteins with predicted intrinsically disordered regions. Several of these families have members highly divergent in sequence, likely to be associated with survival and interactions within the host and the vector. The sequence of the A. platys genome can benefit future studies regarding invasion, survival, and pathogenesis of Anaplasma species, while paving the way for the better design of treatment and prevention strategies against these neglected intracellular pathogens.We have assembled the first genome draft of Anaplasma platys, an obligate intracellular rickettsia, and the only known bacterial pathogen infecting canine platelets. A. platys is a not-yet-cultivated bacterium that causes infectious cyclic canine thrombocytopenia, a potentially fatal disease in dogs. Despite its global distribution and veterinary relevance, no genome sequence has been published so far for this pathogen. Here, we used a strategy based on metagenome assembly to generate a draft of the A. platys genome using the blood of an infected dog. The assembled draft is similar to other Anaplasma genomes in size, gene content, and synteny. Notable differences are the apparent absence of rbfA, a gene encoding a 30S ribosome-binding factor acting as a cold-shock protein, as well as two genes involved in biotin metabolism. We also observed differences associated with expanded gene families, including those encoding outer membrane proteins, a type IV secretion system, ankyrin repeat-containing proteins, and proteins with predicted intrinsically disordered regions. Several of these families have members highly divergent in sequence, likely to be associated with survival and interactions within the host and the vector. The sequence of the A. platys genome can benefit future studies regarding invasion, survival, and pathogenesis of Anaplasma species, while paving the way for the better design of treatment and prevention strategies against these neglected intracellular pathogens

VianniaTopes: a database of predicted immunogenic peptides for Leishmania (Viannia) species

Leishmania is a protozoan parasite causing several disease presentations collectively known as leishmaniasis. Pathogenic species of Leishmania are divided into two subgenera, L. (Leishmania) and L. (Viannia). Species belonging to the Viannia subgenus have only been reported in Central and South America. These species predominantly cause cutaneous leishmaniasis, but in some cases, parasites can migrate to the nasopharyngeal area and cause a highly disfiguring mucocutaneous presentation. Despite intensive efforts, no effective antileishmanial vaccine is available for use in humans, although a few candidates mainly designed for L. (Leishmania) species are now in clinical trials. After sequencing the genome of Leishmania panamensis, we noticed a high degree of sequence divergence among several orthologous proteins from both subgenera. Consequently, some of the previously published candidates may not work properly for species of the Viannia subgenus. To help in vaccine design, we predicted CD4+ and CD8+ T cell epitopes in the theoretical proteomes of four strains belonging to the Viannia subgenus. Prediction was performed with at least two independent bioinformatics tools, using the most frequent human major histocompatibility complex (MHC) class I and class II alleles in the affected geographic area. Although predictions resulted in millions of peptides, relatively few of them were predicted to bind to several MHC alleles and can therefore be considered promiscuous epitopes. Comparison of our results to previous applications to species of the Leishmania subgenus confirmed that approximately half of the reported candidates are not present in Viannia proteins with a threshold of 80% sequence similarity and coverage. However, our prediction methodology was able to predict 70–100% of the candidates that could be found in Viannia. All the prediction data generated in this study are publicly available in an interactive database called VianniaTopes.Leishmania is a protozoan parasite causing several disease presentations collectively known as leishmaniasis. Pathogenic species of Leishmania are divided into two subgenera, L. (Leishmania) and L. (Viannia). Species belonging to the Viannia subgenus have only been reported in Central and South America. These species predominantly cause cutaneous leishmaniasis, but in some cases, parasites can migrate to the nasopharyngeal area and cause a highly disfiguring mucocutaneous presentation. Despite intensive efforts, no effective antileishmanial vaccine is available for use in humans, although a few candidates mainly designed for L. (Leishmania) species are now in clinical trials. After sequencing the genome of Leishmania panamensis, we noticed a high degree of sequence divergence among several orthologous proteins from both subgenera. Consequently, some of the previously published candidates may not work properly for species of the Viannia subgenus. To help in vaccine design, we predicted CD4+ and CD8+ T cell epitopes in the theoretical proteomes of four strains belonging to the Viannia subgenus. Prediction was performed with at least two independent bioinformatics tools, using the most frequent human major histocompatibility complex (MHC) class I and class II alleles in the affected geographic area. Although predictions resulted in millions of peptides, relatively few of them were predicted to bind to several MHC alleles and can therefore be considered promiscuous epitopes. Comparison of our results to previous applications to species of the Leishmania subgenus confirmed that approximately half of the reported candidates are not present in Viannia proteins with a threshold of 80% sequence similarity and coverage. However, our prediction methodology was able to predict 70–100% of the candidates that could be found in Viannia. All the prediction data generated in this study are publicly available in an interactive database called VianniaTopes

Complete Genome Sequence of a Virulent Leptospira interrogans Serovar Copenhageni Strain, Assembled with a Combination of Nanopore and Illumina Reads

Here, we present the complete genome sequence of a highly virulent Leptospira interrogans serovar Copenhageni strain isolated from a dog with severe leptospirosis. In this work, a gapless genome draft was assembled with a combination of Nanopore and Illumina data of relatively low coverage.Here, we present the complete genome sequence of a highly virulent Leptospira interrogans serovar Copenhageni strain isolated from a dog with severe leptospirosis. In this work, a gapless genome draft was assembled with a combination of Nanopore and Illumina data of relatively low coverage

Multiplicadores domésticos “SAMEA†en un modelo multisectorial económico y ambiental de España

En este trabajo se realiza una aplicación de los denominados sistemas híbridos, donde se integra información de distinta naturaleza (en este caso, económica, social y medioambiental Para ello se elabora una matriz de contabilidad social y medioambiental (SAMEA) de España para el año 2000, aplicada al recurso agua y las emisiones de gases efecto invernadero, estimada a partir de datos oficiales del INE. Este sistema se utiliza como pieza central de un modelo multisectorial del funcionamiento económico y medioambiental y se calculan los “multiplicadores domésticos SAMEAâ€. Estos multiplicadores muestran los efectos de las diferentes actividades en la producción total de la economía y en el deterioro medioambiental.Modelos Input-Output, evaluación de efectos medioambientales, emisiones contaminantes, cuentas ambientales, Agricultural and Food Policy, C68, Q51, Q52, Q56.,

Genome-wide discovery and development of polymorphic microsatellites from Leishmania panamensis parasites circulating in central Panama

The parasite Leishmania panamensis is the main cause of leishmaniasis in Panama. The disease is largely uncontrolled, with a rising incidence and no appropriate control measures. While microsatellites are considered some of the best genetic markers to study population genetics and molecular epidemiology in these and other parasites, none has been developed for L. panamensisThe parasite Leishmania panamensis is the main cause of leishmaniasis in Panama. The disease is largely uncontrolled, with a rising incidence and no appropriate control measures. While microsatellites are considered some of the best genetic markers to study population genetics and molecular epidemiology in these and other parasites, none has been developed for L. panamensi

La SAMEA y la eficiencia económica y ambiental en España

This paper aims to show the utility of the so-called Social Accounting Matrix and Environmental Accounts (SAMEA) for economic and environmental efficiency analysis. The article use the SAMEA for Spain in 2000, applied to the water resource and to greenhouses gases emissions. The estimation has been made from official data of the INE. This matrix is used like central core of a multisectorial model of the economic and environmental performance, and is calculated, what have been called "domestics multipliers SAMEA" and their decomposition into characteristic, direct, indirect and induced effects. These multipliers show some of the valuation economic and environmental efficiency. Also, is made an application of these multipliers that allows to appreciate that there is no causal interrelation between the sectors with a higher economic backward linkages and higher environmental deterioration backward linkages.Input-Output Models; Evaluation of Environmental Effects; Air Pollution; Environmental Accounting

La figura del orientador como agente del cambio educativo e innovador: introducción de la impresión 3D como recurso

La transición de la Sociedad de la Información a la Sociedad del Conocimiento ha revolucionado todos los ámbitos existentes a nuestro alrededor, especialmente el de la educación, donde ha originado un cambio de paradigma educativo que tiene que luchar por adaptarse a estas variaciones. De forma paralela a la educación, la orientación educativa también está cambiando. Los antiguos modelos de orientación centrados en el diagnóstico se han transformado en redes colaborativas de profesionales en las escuelas. Del mismo modo, en la educación, hemos dejado atrás aquellos tiempos en los que trabajábamos con lápiz y papel, para abrirle las puertas a la tecnología digital, y aprovechar todos los beneficios y recursos que nos brinda. El profesional de la orientación, en su papel como agente de cambio, tiene el compromiso de garantizar esta transformación tecnológica en las escuelas, asesorando y guiando en el cambio a toda la comunidad educativa. La tecnología de la impresión 3D está abriéndose camino en muchos campos, siendo uno de ellos la educación. Por este motivo, se pretende explorar sus beneficios y posibilidades, así como crear una guía de actividades que faciliten su inclusión en las aulas y mostrar cómo en otras escuelas han adoptado esta tecnología en la creación de proyectos de diversa índole. A través de una revisión bibliográfica se ha trabajado en emparejar dos conceptos aparentemente inconexos, como son la orientación educativa y la impresión 3D. Para ello, se ha hecho hincapié en la figura del profesional de la orientación como líder de un proceso de cambio innovador, con el objetivo de acompañar a los centros educativos en la adopción de un nuevo recurso tecnológico, mostrando sus beneficios y posibilidades de aplicación. <br /

Mixing and m(q) dependence of axial vector mesons in the Coulomb gauge QCD model.

We discuss pure q (q) over bar axial-vector mesons in the Tamm-Dancoff approximation of the Coulomb-gauge QCD model from North Carolina State University. While recent studies have put emphasis in configuration mixing with open meson-meson channels, we here concentrate on the simpler closed-channel problem and follow the 1(+) mixing through a wide range of quark masses. We also examine their radial excitations and discuss with them the concept of insensitivity to chiral symmetry breaking

The genome of Leishmania panamensis: insights into genomics of the L. (Viannia) subgenus.

Kinetoplastid parasites of the Leishmania genus cause several forms of leishmaniasis. Leishmania species pathogenic to human are separated into two subgenera, Leishmania (Leishmania) and L. (Viannia). Species from the Viannia subgenus cause predominantly cutaneous leishmaniasis in Central and South America, occasionally leading to more severe clinical presentations. Although the genomes of several species of Leishmania have been sequenced to date, only one belongs to this rather different subgenus. Here we explore the unique features of the Viannia subgenus by sequencing and analyzing the genome of L. (Viannia) panamensis. Against a background of conservation in gene content and synteny, we found key differences at the genomic level that may explain the occurrence of molecular processes involving nucleic acid manipulation and differential modification of surface glycoconjugates. These differences may in part explain some phenotypic characteristics of the Viannia parasites, including their increased adaptive capacity and enhanced metastatic ability.Kinetoplastid parasites of the Leishmania genus cause several forms of leishmaniasis. Leishmania species pathogenic to human are separated into two subgenera, Leishmania (Leishmania) and L. (Viannia). Species from the Viannia subgenus cause predominantly cutaneous leishmaniasis in Central and South America, occasionally leading to more severe clinical presentations. Although the genomes of several species of Leishmania have been sequenced to date, only one belongs to this rather different subgenus. Here we explore the unique features of the Viannia subgenus by sequencing and analyzing the genome of L. (Viannia) panamensis. Against a background of conservation in gene content and synteny, we found key differences at the genomic level that may explain the occurrence of molecular processes involving nucleic acid manipulation and differential modification of surface glycoconjugates. These differences may in part explain some phenotypic characteristics of the Viannia parasites, including their increased adaptive capacity and enhanced metastatic ability