1 research outputs found
Strategy To Enhance the Wettability of Bioacive Paper-Based Sensors
This paper reports a potential method that can restore
the wettability of bioactive paper-based sensors while maintaining
their bioactivity. This study is driven by the need to increase the
wettability of the antibody-loaded blood typing paper devices in order
to increase the blood typing assaying speed using such paper devices.
Plasma treatment is used to improve the wettability of bioactive paper;
the protective effect of bovine serum albumin (BSA) to biomolecules
against plasma deactivation is investigated. In the first stage, horseradish
peroxidase (HRP) was used as a model biomolecule, because of the convenience
of its quantifiable colorimetric reaction with a substrate. By using
this protection approach, the inactivation of biomolecules on paper
during the plasma treatment is significantly slowed down. This approach
enables plasma treatment to be used for fabricating paper-based bioactive
sensors to achieve strong wettability for rapid penetration of liquid
samples or reagents. Finally, we demonstrate the use of plasma treatment
to increase the wettability of antibody treated blood typing paper.
After the treatment, the blood typing paper becomes highly wettable;
it allows much faster penetration of blood samples into the plasma
treated testing paper. Antibodies on the paper are still sufficiently
active for blood typing and can report patients’ blood type
accurately