El Glu regula traduccionalmente a la proteína BMAL 1 en cultivos primarios de BGC

El glutamato, el principal aminoácido excitador, activa una gran variedad de cascadas de transducción de señales. Este neurotransmisor está involucrado en la sincronización fótica de los ritmos circadianos, los cuales regulan funciones fisiológicas y conductuales. El reloj circadiano en los vertebrados, el núcleo supraquiasmático, está basado en un asa de retroalimentación de transcripción-traducción, en el cual la proteína BMAL1 actúa como un activador transcripcional de otros genes reloj. Esta proteína se expresa en casi todas las neuronas del núcleo supraquiasmático, y otras regiones cerebrales y periféricas. El objetivo del estudio fue investigar la función del glutamato en los mecanismos moleculares implicados en los procesos de transcripción/traducción de la proteína BMAL1

Current Opinion on the Use of c-Fos in Neuroscience

For years, the biochemical processes that are triggered by harmful and non-harmful stimuli at the central nervous system level have been extensively studied by the scientific community through numerous techniques and animal models. For example, one of these techniques is the use of immediate expression genes, which is a useful, accessible, and reliable method for observing and quantifying cell activation. It has been shown that both the c-fos gene and its protein c-Fos have rapid activation after stimulus, with the length of time that they remain active depending on the type of stimulus and the activation time depending on the stimulus and the structure studied. Fos requires the participation of other genes (such as c-jun) for its expression (during hetero-dimer forming). c-Fos dimerizes with c-Jun protein to form factor AP-1, which promotes the transcription of various genes. The production and removal of c-Fos is part of cellular homeostasis, but its overexpression results in increased cell proliferation. Although Fos has been used as a marker of cellular activity since the 1990s, which molecular mechanism participates in the regulation of the expression of this protein is still unknown because the gene and the protein are not specific to neurons or glial cells. For these reasons, this work has the objective of gathering information about this protein and its use in neuroscience