Fluoro Jade C staining of lesion areas in brain sections.

<p>All sections were taken from mice sacrificed at 14 days post-TBI. Green is FJC and blue is DAPI )control for equal numbers of cell nuclei. (A) Sham-TBI control mouse; (B) real-TBI sham Tx mouse. (C) TBI mouse treated with 1 laser Tx. (C) TBI mouse treated with 3 laser Tx (E) TBI mouse treated with 14 laser Tx (F) Mean green staining calculated with Image J from mice (n = 2–3) in the groups in panels A–E. # P<0.05 vs TBI sham; * P<0.05 vs 14 laser Tx. One way ANOVA.</p

BrdU staining of lesion areas in brain sections.

<p>All sections were taken from mice sacrificed at 28 days post-TBI. Red is BrdU and blue is DAPI (control for equal numbers of cell nuclei). (A) Sham control mouse; (B) TBI sham untreated mouse. (C) TBI mouse treated with 1 laser Tx. (C) TBI mouse treated with 3 laser Tx (E) TBI mouse treated with 14 laser Tx (F) Mean (n = 6) red staining calculated with Image J from mice (n = 6) in the groups in panels A–E. *** P<0.001 vs TBI sham; # P<0.05 vs 1 laser Tx; <a href="mailto:@@" target="_blank">@@</a> P<0.01 vs 14 laser Tx. One way ANOVA.</p

Wire grip and motion score for TBI mice.

<p>Mice are assessed the score for the best level they reach.</p

Neurological Severity Score (NSS) for TBI Mice.

<p>Mice are awarded 1 point for each failure to complete a task.</p

WGMT scores of the mice.

<p>Mean (n = 8–14) WGMT scores measured over 4 weeks, of mice in 9 groups consisting of sham TBI mice, sham control mice, 810-nm laser treated TBI mice (18 J/cm² delivered at 25 mW/cm²). (A) Mice and controls given 1 laser Tx or 1 sham Tx at 4 hours post TBI; (B) Mice and controls given 3 daily laser Tx or 3 sham Tx; (C) Mice and controls given 14 daily laser Tx or 14 sham Tx. (D) Mean areas under curve of WGMT time courses from mice in different groups. P values given were determined by one-way ANOVA.</p

Effect of PDT doses on MRSA arthritis. a

<p>: Time course series of bioluminescent images after therapeutic PDT (Th-PDT) in each irradiation energy group. <b>b</b>: A line graph of time courses of bioluminescent intensity after Th-PDT in each irradiation energy group. <b>c</b>: Comparison of the area under the RLU curve (AUC) indicated in b. <i>n</i> = 5 each. *<i>P</i><0.05, **<i>P</i><0.01.</p

Effect of Pre-PDT on MRSA arthritis. a

<p>: Time course series of bioluminescent images after preventive PDT (Pre-PDT) in each group. <b>b</b>: A line graph of time courses of bioluminescent intensity after Pre-PDT in each group. <b>c</b>: Comparison of the area under the RLU curve (AUC) of the data indicated in b. <i>n</i> = 5 each. *<i>P</i><0.05, **<i>P</i><0.01.</p

MRSA arthritis model followed by bioluminescence imaging.

<p>(a) Time course of the bioluminescent signal of MRSA arthritis models. (b) Images of bioluminescence in the model using microparticle (MP+ group) and in the model without microparticle (MP- group). <i>n</i> = 5 each. *<i>P</i><0.05.</p

Time course of intrarticular leukocytes.

<p>Comparison of time courses of intraarticular leukocyte counts in the Pre-PDT (−1d) group and the PS-IR- group. <i>n</i> = 5 each. *<i>P</i><0.05, **<i>P</i><0.01.</p

Histology of knees from Pre-PDT treated mice.

<p>Histopathological images of the intraarticular tissues of the knee joint in the preventive PDT (Pre-PDT) group and the PS-IR- group (hematoxylin-eosin staining and immunostaining using an anti-GR-1 antibody). Bar = 50 µm.</p