7 research outputs found
Three New Hg(II) Complexes Constructed From Mercapto- and Amino-Containing Triazole Ligands: Syntheses, Crystal Structures, and Luminescent Properties
<div><p>Three new mercury(II) complexes [HgI<sub>2</sub>(3-aptt)<sub>2</sub>] (1), [Hg(4-aptt)<sub>2</sub>] (2), and [Hg(a-mtt)<sub>2</sub>]·H<sub>2</sub>O (3) were synthesized based on three structurally related ligands: 4-amino-3-(3-pyridyl)-1H-1,2,4-triazole-5-thione (3-Haptt), 4-amino-3-(4-pyridyl)-5-mercapto-1,2,4-triazole (4-Haptt), and 4-amino-3-methyl-1,2,4-triazoline-5-thiadone (a-Hmtt). The structures of complexes 1–3 were determined by single-crystal X-ray diffraction techniques and further characterized by elemental analyses and IR spectra. The results reveal that these three complexes take mononuclear structures. And although various weak interactions, including N‒H···I and N···H‒O H-bondings as well as N<sub>trz</sub>···N<sub>py</sub> and π···π stacking, play significant roles in crystal structures, just these weak interactions link the mononuclear units into higher dimensional supramolecular networks. Their thermal stabilities and the solid state luminescent properties have also been discussed.</p></div
Cardiac specific deletion of KLF15 alters tissue and plasma levels of free fatty acids and triglycerides.
<p>Cardiac FFA (A) and TG (B) levels in control (MHC-Cre) vs. KF15-cKO following 48 hours fasting, (n = 5), *P<0.05 vs. Cre Fed, **P<0.05 vs. CKO Fed, # P<0.05 vs. Cre Fast. Plasma FFA (C) and TG (D) levels in control (MHC-Cre) vs. KLF15-cKO following 48 hours fasting, (n = 5), *P<0.05 vs. Cre Fed, **P<0.05 vs. CKO Fed, # P<0.05 vs. Cre Fast.</p
Short-chain diet rescues the KLF15-dependent attenuation of cardiac function in response to fasting.
<p>(A) qPCR analysis of expression of transporter genes in MHC-Cre vs. KLF15-cKO under fed vs. 48 hour fasting conditions. *P<0.05 vs. Cre Fed, **P<0.05 vs. CKO Fed, # P<0.05 vs. Cre Fast. Values normalized to <i>Ppib</i>. (B) <i>Slc25a20</i> expression (qPCR) in MHC-Cre vs. KLF15-cKO under fed vs. 48 hour fasting conditions. *P<0.05 vs. Cre Fed, **P<0.05 vs. CKO Fed, # P<0.05 vs. Cre Fast. Values normalized to <i>Ppib</i>. (C) Western blot analysis of CACT levels in MHC-Cre vs KLF15-cKO under fed and 48 hour fasting conditions. α-tubulin used as loading control. (D) Quantification of data in C (n = 3 per group). Two-tailed Student's <i>t</i>-test for unpaired data was used. *P<0.05. (E) Left ventricular fractional shortening from echocardiography performed in control (MHC-Cre) vs. KLF15-cKO under fed vs. 48 hours fasting conditions following 10 weeks of short-chain fatty acid diet, (n = 10). (F) Representative echocardiography image from MHC-Cre vs. KLF15-cKO following 48 hours fasting and 10 weeks of short-chain fatty acid diet. (G) Tabular representation of echocardiography data in MHC-Cre vs. KLF15-cKO under fed vs. 48 hour fasting conditions following 10 weeks of short-chain fatty acid diet.</p
Cardiac specific deletion of KLF15 alters lipid profile.
<p>Metabolomic analysis of long chain acylcarnitines in cardiac tissue from control (MHC-Cre) vs. KLF15-cKO with and without 48 hour fast, (n = 5), *P<0.05 by one-way analysis of variance (ANOVA) with the Tukey post hoc test.</p
Cardiac KLF15 is required for the heart’s functional adaptation in response to fasting.
<p>(A) Left ventricular fractional shortening from echocardiography performed in control (MHC-Cre) vs KLF15-cKO under fed vs. 48 hours fasting conditions, (n = 5), *P<0.05 vs. MHC-Cre Fast. (B) Representative echocardiography image from MHC-Cre vs. KLF15-cKO following a 48 hour fast. (C) Tabular representation of echocardiography data in MHC-Cre vs. KLF15-cKO under fed vs. 48 hour fasting conditions.</p
Change in long chain acylcarnitine profiles after loss of cardiac KLF15 expression.
<p>Change in long chain acylcarnitine profiles after loss of cardiac KLF15 expression.</p
Systemic KLF15 is required for the heart’s functional adaptation in response to fasting.
<p>(A) Left ventricular fractional shortening from echocardiography performed in wild-type (WT) vs. systemic <i>Klf15</i>-null (<i>Klf15-/-</i>) under fed vs. 48 hours fasting conditions, (n = 5), *P,0.05 vs. WT Fast. (B) Representative echocardiography image from WT vs. <i>Klf15-/-</i> following a 48 hour fast. (C) Tabular representation of echocardiography data in WT vs. <i>Klf15-/-</i> under fed vs. 48 hour fasting conditions.</p