Additional file 1: Figure S1. of Glycerolipidome responses to freezing- and chilling-induced injuries: examples in Arabidopsis and rice

Hierarchical average linkage clustering of lipid molecular species relative contents (mol%). Table S1. Levels of galactolipid species in leaves of Arabidopsis and rice after various low-temperature treatments. Table S2. Component matrix of PCA analysis. Table S3. Lipid ratios of Arabidopsis and rice after various low-temperature treatments. Table S4. Levels of PG molecular species in leaves of Arabidopsis and rice after various low-temperature treatments. Table S5. Levels of PA molecular species in leaves of Arabidopsis and rice after various low-temperature treatments. (DOC 446 kb

Supplementary document for Large Scale Plasmonic Nanodisk Array as Biosensing Platform Fabricated by Transfer Nanoprinting - 6609524.pdf

Calculated electric field amplitude profile and SEM imag

Immunostaining analyses of grafted MSCs in a rat model of Tourette’s syndrome at 4 wk after transplantation.

<p>Grafted MSCs are identified by BrdU staining. (A) Grafted cells are double-labeled with anti-BrdU (purple) and anti-Nestin (red). (B) Some grafted MSCs differentiated into neurons, as evidenced by double-labeling with BrdU and MAP-2. (C) Grafted cells differentiated into astrocytes, which coexpressed BrdU and GFAP. Scale bar: 20 µm. Arrows represent transplanted MSCs that differentiated into different types of neural cells.</p

Sensitivity analysis of rs4444235 and colorectal cancer risk.

<p>Abbreviations: OR, odds ratio; 95% CI, 95% confidence interval; OR_G, generalized OR.</p><p>*<i>P</i> values for heterogeneity were calculated by the Cochran’s χ² based Q test.</p

The Common Variant rs4444235 near <i>BMP4</i> Confers Genetic Susceptibility of Colorectal Cancer: An Updated Meta-Analysis Based on a Comprehensive Statistical Strategy

<div><p>Objective</p><p>We performed an updated meta-analysis, using a comprehensive strategy of a logistic regression and a model-free approach, to evaluate more precisely the role of the rs4444235 variant near the Bone morphogenetic protein-4 (<i>BMP4</i>) gene in susceptibility to colorectal cancer (CRC).</p><p>Methods</p><p>A total of 19 studies with 28770 cases and 28234 controls were included. Metagen system with logistic regression was applied to choose the most plausible genetic model for rs4444235. Generalized odds ratio (OR_G) metric was used to provide a global test of relationship between rs4444235 and CRC risk.</p><p>Results</p><p>Metagen analysis suggested the rs4444235 fitted best to an additive model. In assessment of the additive model, heterogeneity was observed (<i>P</i> = 0.059, <i>I²</i> = 36.1), and pooled per-allele OR was 1.08 (95% CI = 1.05–1.11). Based on the model-free approach, pooled OR_G was 1.09 (95% CI = 1.05–1.14) under a random-effect model. Stratified analyses suggested heterogeneity could be in part explained by population ethnicity, study design, sources of controls, and sample size. Sensitivity analysis further supported the robust stability of the current results, by showing similar pooled estimates before and after sequential removal of each study.</p><p>Conclusions</p><p>This meta-analysis provides a robust estimate of the positive association between the rs4444235 and CRC risk and further emphasizes the importance of the rs4444235 in CRC risk prediction.</p></div

Flow cytometry analysis of MSCs.

<p>Cells were cultured for 3–4 passages, harvested, and analyzed by a flow cytometry system. The y-axis represents the number of cells, and the x-axis represents CD29, CD-44, CD105, CD106, CD34, and CD45.</p

The amounts of dopamine (DA) in striatum homogenate (Figure 4) were analyzed at the end of the test by high-performance liquid chromatography with electrochemical detection in 3 groups (n = 16).

<p>The amounts of dopamine (DA) in striatum homogenate (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0062198#pone-0062198-g004" target="_blank">Figure 4</a>) were analyzed at the end of the test by high-performance liquid chromatography with electrochemical detection in 3 groups (n = 16).</p

Stereotypy counts were recorded for 30 min at different time points in 3 groups.

<p>Scores are higher in the Tourette syndrome (TS) group than those in the control group. TS rats with mesenchymal stem cell (MSC) grafts exhibited decreased stereotypic behaviors compared with TS+vehicle rats. <i>P</i><0.05.</p

Characteristics of included studies in the meta-analysis of rs4444235 and colorectal cancer.

<p>Abbreviations: GWAS, genome-wide association study; MAF, minor allele frequency (the C allele of rs4444235).</p

Meta-analysis of rs4444235 and colorectal cancer risk.

<p>Abbreviations: GWAS, genome-wide association study; OR, odds ratio; 95% CI, 95% confidence interval; OR_G, generalized OR.</p