TAGAP expression influences CD4+ T cell differentiation, immune infiltration, and cytotoxicity in LUAD through the STAT pathway: implications for immunotherapy

BackgroundT-cell Activation GTPase Activating Protein (TAGAP) plays a role in immune cell regulation. This study aimed to investigate TAGAP’s expression and its potential impact on CD4+ T cell function and prognosis in lung adenocarcinoma (LUAD).MethodsWe analyzed TAGAP expression and its correlation with immune infiltration and clinical data in LUAD patients using multiple datasets, including The Cancer Genome Atlas (TCGA-LUAD), Gene Expression Omnibus (GEO), and scRNA-seq datasets. In vitro and in vivo experiments were conducted to explore the role of TAGAP in CD4+ T cell function, chemotaxis, and cytotoxicity.ResultsTAGAP expression was significantly lower in LUAD tissues compared to normal tissues, and high TAGAP expression correlated with better prognosis in LUAD patients. TAGAP was positively correlated with immune/stromal/ESTIMATE scores and immune cell infiltration in LUAD. Single-cell RNA sequencing revealed that TAGAP was primarily distributed in CD4+/CD8+ T cells. In vitro experiments showed that TAGAP overexpression enhanced CD4+ T cell cytotoxicity, proliferation, and chemotaxis. Gene Set Enrichment Analysis (GSEA) indicated that TAGAP was enriched in the JAK-STAT signaling pathway. In vivo experiments in a xenograft tumor model demonstrated that TAGAP overexpression suppressed tumor growth and promoted CD4+ T cell cytotoxicity.ConclusionsTAGAP influences CD4+ T cell differentiation and function in LUAD through the STAT pathway, promoting immune infiltration and cytotoxicity. This study provides a scientific basis for developing novel LUAD immunotherapy strategies and exploring new therapeutic targets

(2S,3R)-3-(2-Bromophenyl)-2-nitro-2,3,6,7-tetrahydro-1-benzofuran-4(5H)-one

The title compound, C14H12BrNO4, has two chiral C atoms. The C atom next to the O atom in the dihydrofuran ring has an S configuration, while the adjacent chiral C atom has an R configuration. The cyclohex-2-enone and dihydrofuran rings both adopt envelope conformations, with the flap atoms (middle CH2 in cyclohex-2-enone and NO2-substituted C in dihydrofuran) lying 0.612 (3) and 0.295 (2) Å, respectively, from the mean plane of the remaining atoms. The dihedral angle between the mean planes of the furan and benzene rings is 80.0 (3)°. In the crystal, the molecules are linked by C—H...O interactions, generating a three-dimensional network

Simulation of Nonseparable Nonstationary Spatially Varying Ground Motions with an Enhanced Interpolation Approximation Approach

An enhanced interpolation approach is developed for simulating nonseparable nonstationary ground motions on the basis of the spectral representation method, which mainly contains two steps of interpolations and an optimization. Firstly, the interpolation technique is utilized to reduce the Cholesky decomposition time of the lagged coherence matrix. The square root of the evolutionary power spectral density is then decoupled into several time and frequency discrete functions using the proper orthogonal decomposition (POD) interpolation technique, which results in the availability of the fast Fourier transform (FFT) technique in the simulation. Compared with existing decoupling schemes, the POD interpolation achieves a significant efficiency improvement with a slight accuracy reduction. Finally, the simulation formula is further optimized to reduce the number of FFT operations. The accuracy and efficiency of this method are verified with the numerical examples of nonstationary ground motions simulation. Results show that the error introduced by two-step interpolations is fairly small and the simulation agrees with the targets very well. Furthermore, the efficiency generating sample function is significantly enhanced

Investigation on the Status Quo of Self-Health Management of Patients with Bipolar Disorder and Analysis of Influencing Factors

In this study, we aim to explore the status quo of self-health management of patients with bipolar disorder and influencing factors. A total of 80 patients with bipolar disorder were included and assigned into the observation group and the control group, with 40 cases per group. The patients in the control group were given general health education, and those in the observation group were given personalized education combined with peer support. It was found that there was no significant difference in gender, age, educational degree, marital status, and living state between the observation group and the control group (P>0.05). There are significant differences with regard to self-management between the two groups. The observation group showed stronger self-care ability, health education level, self-protection ability, and emotional control ability than the control group (P0.05). After the intervention, the HAMD scores were lower in the observation group than in the control group (P<0.05), suggesting an alleviation in manic and depressive episodes in the observation group. It was also revealed that the observation group exhibited lower Functioning Assessment Short Test (FAST) scores in all aspects than the control group, suggesting personalized education combined with peer support could significantly improve the quality of life of patients with bipolar disorder. Self-health management ability of the patients was regarded as the dependent variable and healthy education level, health literacy, and objective support as the independent variables, and the regression model was performed. The results show that information acquisition ability, communication and interaction ability, objective social support, subjective social support, and utilization of social support can all affect the self-health management ability of patients. Personalized education combined with peer support can effectively alleviate the condition of patients with bipolar disorder, improve the degree of overall functional impairment, enhance the patient’s self-management ability, and promote their recovery. It is worthy of promotion and application. In conclusion, information acquisition ability, communication and interaction ability, objective social support, subjective social support, and utilization of social support can all affect the self-health management ability of patients

Influence of Phases of Coherence Functions on the Wind Field Simulation Using Spectral Representation Method

The wave passage effect is a measure of the wave passage delay due to the apparent velocity of waves, which is one of spatially varying properties of multivariate random processes. The phase of coherence function reflects the wave passage effect of wind fields. In the wind field, simulation by the spectral representation method, the classical phase formula, is not rigorous. This may affect the accuracy of simulation results and even cause incorrect simulations. In this study, the influences of the phase on stationary and nonstationary wind field simulations are researched and discussed in detail. Two schemes containing the classical phase formula and the separated phase scheme are compared in four types of wind field simulation. The qualitative analysis based on theoretical correlation function formula is first made to study the influence of the phase. Then, four numerical examples are utilized to quantitatively study the magnitude of the influence on the sample time history and correlation function of the simulated wind field. Results show that the classical phase formula will result in considerable simulation error for all four types of wind fields because it cannot completely represent the phase angle of a complex number

Simulation of Nonseparable Nonstationary Spatially Varying Ground Motions with an Enhanced Interpolation Approximation Approach

An enhanced interpolation approach is developed for simulating nonseparable nonstationary ground motions on the basis of the spectral representation method, which mainly contains two steps of interpolations and an optimization. Firstly, the interpolation technique is utilized to reduce the Cholesky decomposition time of the lagged coherence matrix. The square root of the evolutionary power spectral density is then decoupled into several time and frequency discrete functions using the proper orthogonal decomposition (POD) interpolation technique, which results in the availability of the fast Fourier transform (FFT) technique in the simulation. Compared with existing decoupling schemes, the POD interpolation achieves a significant efficiency improvement with a slight accuracy reduction. Finally, the simulation formula is further optimized to reduce the number of FFT operations. The accuracy and efficiency of this method are verified with the numerical examples of nonstationary ground motions simulation. Results show that the error introduced by two-step interpolations is fairly small and the simulation agrees with the targets very well. Furthermore, the efficiency generating sample function is significantly enhanced

Enhanced performance of microbial fuel cells by using MnO2/Halloysite nanotubes to modify carbon cloth anodes

The modification of anode materials is important to enhance the power generation of MFCs (microbial fuel cells). A novel and cost-effective modified anode that is fabricated by dispersing manganese dioxide (MnO2) and HNTs (Halloysite nanotubes) on carbon cloth to improve the MFCs' power production was reported. The results show that the MnO2/HNT anodes acquire more bacteria and provide greater kinetic activity and power density compared to the unmodified anode. Among all modified anodes, 75 wt% MnO2/HNT exhibits the highest electrochemical performance. The maximum power density is 767.3 mWm(-2), which 21.6 higher than the unmodified anode (631 mW/m(2)). Besides, CE (Coulombic efficiency) was improved 20.7, indicating that more chemical energy transformed to electricity. XRD (X-Ray powder diffraction) and FTIR (Fourier transform infrared spectroscopy) are used to characterize the structure and functional groups of the anode. CV (cyclic voltammetry) scans and SEM (scanning electron microscope) images demonstrate that the measured power density is associated with the attachment of bacteria, the microorganism morphology differed between the modified and the original anode. These findings demonstrate that MnO2/FINT nanocomposites can alter the characteristics of carbon cloth anodes to effectively modify the anode for practical MFC applications. (C) 2016 Elsevier Ltd. All rights reserved.National Natural Science Foundation of China [21106072, 51172107]; Research Fund for the Doctoral Program of Higher Education of China [20113221110004]; Key Projects in the National Science & Technology Pillar Program of China [2012BAE01B03]Available online 28 May 2016. 24 Month Embargo.This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [email protected]

A systematic review and meta-analysis of the benefits of a gluten-free diet and/or casein-free diet for children with autism spectrum disorder

ContextIt has been suggested that a gluten-free and casein-free (GFCF) diet may alleviate the symptoms of autism spectrum disorder (ASD) and facilitate neurodevelopment of children with ASD. Studies to date have been inconclusive.ObjectiveThis study aimed to evaluate (through quantitative meta-analysis) the efficacy and safety of a GFCF diet for children with ASD. To our knowledge, this is the first time such an analysis has been carried out.Data sourcesEight electronic databases were searched, from the establishment of each database up to March 27, 2020: PubMed, Web of Science, Embase (Ovid), PsycINFO (Ovid), Cochrane Library, CNKI, Wanfang, and VIP databases.Data extractionTwo authors independently performed the data extraction and risk-of-bias assessment.Data analysisA quantitative meta-analysis was performed with standard procedures by using Stata SE 15 software. Within the total of 8 studies, with 297 participants, 5 studies reported significant reductions in stereotypical behaviors [standard mean difference (SMD) = -0.41, 95% confidence interval (CI): -0.68 to -0.15], and 3 studies reported improvements in cognition (SMD = -0.46, 95% CI: -0.91 to -0.01) following GFCF dietary intervention . No statistically significant changes were observed in other symptomatic categories (all P &gt; 0.05).ConclusionThe current meta-analysis showed that a GFCF diet can reduce stereotypical behaviors and improve the cognition of children with ASD. Though most of the included studies were single-blind, the benefits of a GFCF diet that have been indicated are promising. Additional studies on a larger scale are warranted.Systematic review registrationPROSPERO registration no. CRD42020177619

A Nitrogen-Rich Covalent Triazine Framework as a Photocatalyst for Hydrogen Production

Covalent triazine frameworks (CTFs) have emerged as new candidate materials in various research areas such as catalysis, gas separation storage, and energy-related organic devices due to their easy functionalization, high thermal and chemical stability, and permanent porosity. Herein, we report the successful synthesis of a CTF rich in cyano groups (CTF-CN) by the solvothermal condensation of 2,3,6,7-tetrabromonapthalene (TBNDA), Na2(1,1-dicyanoethene-2,2-dithiolate), and 1,3,5-tris-(4-aminophenyl)-triazine (TAPT) at 120°C. XRD, SEM, and TEM characterization studies revealed CTF-CN to be amorphous and composed of ultrathin 2D sheets. CTF-CN possessed strong absorption at visible wavelengths, with UV-vis measurements suggesting a band gap energy in the range 2.7-2.9 eV. A 5 wt.% Pt/CTF-CN was found to be a promising photocatalyst for hydrogen production, affording a rate of 487.6 μmol g-1 h-1 in a H2O/TEOA/CH3OH solution under visible light. The photocatalytic activity of CTF-CN was benchmarked against g-C3N4 for meaningful assessment of performance. Importantly, the 5 wt.% Pt/CTF-CN photocatalyst exhibited excellent thermal and photocatalytic stability. Further, as a nitrogen-rich porous 2D material, CTF-CN represents a potential platform for the development of novel electrode material for fuel cells and metal ion batteries