In vivo trafficking of endogenous opioid receptors

Studies on trafficking of endogenous opioid receptors in vivo are subject of the present review. In many of the in vivo studies, the use of semi-quantitative immuno-electron microscopy is the approach of choice. Endogenous opioid receptors display differential subcellular distributions with μ opioid receptor (MOPR) being mostly present on the plasma membrane and δ- and κ-opioid receptors (DOPR and KOPR, respectively) having a significant intracellular pool. Etorphine and DAMGO cause endocytosis of the MOPR, but morphine does not, except in some dendrites. Interestingly, chronic inflammatory pain and morphine treatment promote trafficking of intracellular DOPR to the cell surface which may account for the enhanced antinociceptive effects of DOPR agonists. KOPR has been reported to be associated with secretory vesicles in the posterior pituitary and translocated to the cell surface upon salt loading along with the release of vasopressin. The study of endogenous opioid receptors using in vivo models has produced some interesting results that could not have been anticipated in vitro. In vivo studies, therefore, are essential to provide insight into the mechanisms underlying opioid receptor regulation

Vertical Structure of Neutrino Dominated Accretion Disks and Neutrino Transport in the disks

We investigate the vertical structure of neutrino dominated accretion disks by self-consistently considering the detailed microphysics, such as the neutrino transport, vertical hydrostatic equilibrium, the conservation of lepton number, as well as the balance between neutrino cooling, advection cooling and viscosity heating. After obtaining the emitting spectra of neutrinos and antineutrinos by solving the one dimensional Boltzmann equation of neutrino and antineutrino transport in the disk, we calculate the neutrino/antineutrino luminosity and their annihilation luminosity. We find that the total neutrino and antineutrino luminosity is about $10^{54}$ ergs/s and their annihilation luminosity is about $5\times10^{51}$ ergs/s with an extreme accretion rate $10 M_{\rm {sun}}$/s and an alpha viscosity $\alpha=0.1$. In addition, we find that the annihilation luminosity is sensitive to the accretion rate and will not exceed $10^{50}$ ergs/s which is not sufficient to power the most fireball of GRBs, if the accretion rate is lower than $1 M_{\rm {sun}}$/s. Therefore, the effects of the spin of black hole or/and the magnetic field in the accretion flow might be introduced to power the central engine of GRBs.Comment: 22 pages, 9 figures, ApJ accepte

Predictive Modeling of Pedestrian Motion Patterns with Bayesian Nonparametrics

For safe navigation in dynamic environments, an autonomous vehicle must be able to identify and predict the future behaviors of other mobile agents. A promising data-driven approach is to learn motion patterns from previous observations using Gaussian process (GP) regression, which are then used for online prediction. GP mixture models have been subsequently proposed for finding the number of motion patterns using GP likelihood as a similarity metric. However, this paper shows that using GP likelihood as a similarity metric can lead to non-intuitive clustering configurations - such as grouping trajectories with a small planar shift with respect to each other into different clusters - and thus produce poor prediction results. In this paper we develop a novel modeling framework, Dirichlet process active region (DPAR), that addresses the deficiencies of the previous GP-based approaches. In particular, with a discretized representation of the environment, we can explicitly account for planar shifts via a max pooling step, and reduce the computational complexity of the statistical inference procedure compared with the GP-based approaches. The proposed algorithm was applied on two real pedestrian trajectory datasets collected using a 3D Velodyne Lidar, and showed 15% improvement in prediction accuracy and 4.2 times reduction in computational time compared with a GP-based algorithm.Ford Motor Compan

In Situ Measurement of the Junction Temperature of Light Emitting Diodes Using a Flexible Micro Temperature Sensor

This investigation aimed to fabricate a flexible micro resistive temperature sensor to measure the junction temperature of a light emitting diode (LED). The junction temperature is typically measured using a thermal resistance measurement approach. This approach is limited in that no standard regulates the timing of data capture. This work presents a micro temperature sensor that can measure temperature stably and continuously, and has the advantages of being lightweight and able to monitor junction temperatures in real time. Micro-electro-mechanical-systems (MEMS) technologies are employed to minimize the size of a temperature sensor that is constructed on a stainless steel foil substrate (SS-304 with 30 μm thickness). A flexible micro resistive temperature sensor can be fixed between the LED chip and the frame. The junction temperature of the LED can be measured from the linear relationship between the temperature and the resistance. The sensitivity of the micro temperature sensor is 0.059 ± 0.004 Ω/°C. The temperature of the commercial CREE® EZ1000 chip is 119.97 °C when it is thermally stable, as measured using the micro temperature sensor; however, it was 126.9 °C, when measured by thermal resistance measurement. The micro temperature sensor can be used to replace thermal resistance measurement and performs reliably

GEC1-kappa Opioid Receptor Binding Involves Hydrophobic Interactions GEC1 has Chaperone-Like Effect

We demonstrated previously that the protein GEC1 (glandular epithelial cell 1) bound to the human κ opioid receptor (hKOPR) and promoted cell surface expression of the receptor by facilitating its trafficking along the secretory pathway. Here we showed that three hKOPR residues (Phe345, Pro346, and Met350) and seven GEC1 residues (Tyr49, Val51, Leu55, Thr56, Val57, Phe60, and Ile64) are indispensable for the interaction. Modeling studies revealed that the interaction was mediated via direct contacts between the kinked hydrophobic fragment in hKOPR C-tail and the curved hydrophobic surface in GEC1 around the S2 β-strand. Intramolecular Leu44-Tyr109 interaction in GEC1 was important, likely by maintaining its structural integrity. Microtubule binding mediated by the GEC1 N-terminal domain was essential for the GEC1 effect. Expression of GEC1 also increased cell surface levels of the GluR1 subunit and the prostaglandin EP3.f receptor, which have FPXXM and FPXM sequences, respectively. With its widespread distribution in the nervous system and its predominantly hydrophobic interactions, GEC1 may have chaperone-like effects for many cell surface proteins along the biosynthesis pathway

Safety and efficacy of etomidate and propofol anesthesia in elderly patients undergoing gastroscopy: A double-blind randomized clinical study

The aim of the present study is to compare the safety, efficacy and cost effectiveness of anesthetic regimens by compound, using etomidate and propofol in elderly patients undergoing gastroscopy. A total of 200 volunteers (65–79 years of age) scheduled for gastroscopy under anesthesia were randomly divided into the following groups: P, propofol (1.5–2.0 mg/kg); E, etomidate (0.15-0.2 mg/kg); P+E, propofol (0.75–1 mg/kg) followed by etomidate (0.075-0.1 mg/kg); and E+P, etomidate (0.075-0.01 mg/kg) followed by propofol (0.75–1 mg/kg). Vital signs and bispectral index were monitored at different time points. Complications, induction and examination time, anesthesia duration, and recovery and discharge time were recorded. At the end of the procedure, the satisfaction of patients, endoscopists and the anesthetist were evaluated. The recovery (6.1±1.2 h) and discharge times (24.8±2.8 h) in group E were significantly longer compared with groups P, P+E and E+P (P<0.05). The occurrence of injection pain in group P+E was significantly higher compared with the other three groups (P<0.05). In addition, the incidence of myoclonus and post-operative nausea and vomiting were significantly higher in group P+E compared with the other three groups (P<0.05). There was no statistical difference among the four groups with regards to the patients' immediate, post-procedure satisfaction (P>0.05). Furthermore, there was no difference in the satisfaction of anesthesia, as evaluated by the anesthetist and endoscopist, among the four groups (P>0.05). The present study demonstrates that anesthesia for gastroscopy in elderly patients can be safely and effectively accomplished using a drug regimen that combines propofol with etomidate. The combined use of propofol and etomidate has unique characteristics which improve hemodynamic stability, cause minimal respiratory depression and less side effects, provide rapid return to full activity and result in high levels of satisfaction

Loop Formulas for Description Logic Programs

Description Logic Programs (dl-programs) proposed by Eiter et al. constitute an elegant yet powerful formalism for the integration of answer set programming with description logics, for the Semantic Web. In this paper, we generalize the notions of completion and loop formulas of logic programs to description logic programs and show that the answer sets of a dl-program can be precisely captured by the models of its completion and loop formulas. Furthermore, we propose a new, alternative semantics for dl-programs, called the {\em canonical answer set semantics}, which is defined by the models of completion that satisfy what are called canonical loop formulas. A desirable property of canonical answer sets is that they are free of circular justifications. Some properties of canonical answer sets are also explored.Comment: 29 pages, 1 figures (in pdf), a short version appeared in ICLP'1