Activation of E-prostanoid 3 receptor in macrophages facilitates cardiac healing after myocardial infarction

Two distinct monocyte (Mo)/macrophage (Mp) subsets (Ly6Clow and Ly6Chigh) orchestrate cardiac recovery process following myocardial infarction (MI). Prostaglandin (PG) E2 is involved in the Mo/Mp-mediated inflammatory response, however, the role of its receptors in Mos/Mps in cardiac healing remains to be determined. Here we show that pharmacological inhibition or gene ablation of the Ep3 receptor in mice suppresses accumulation of Ly6Clow Mos/Mps in infarcted hearts. Ep3 deletion in Mos/Mps markedly attenuates healing after MI by reducing neovascularization in peri-infarct zones. Ep3 deficiency diminishes CX3C chemokine receptor 1 (CX3CR1) expression and vascular endothelial growth factor (VEGF) secretion in Mos/Mps by suppressing TGFβ1 signalling and subsequently inhibits Ly6Clow Mos/Mps migration and angiogenesis. Targeted overexpression of Ep3 receptors in Mos/Mps improves wound healing by enhancing angiogenesis. Thus, the PGE2/Ep3 axis promotes cardiac healing after MI by activating reparative Ly6Clow Mos/Mps, indicating that Ep3 receptor activation may be a promising therapeutic target for acute MI

PKA regulatory IIα subunit is essential for PGD2-mediated resolution of inflammation

journal articl

Niacin ameliorates ulcerative colitis via prostaglandin D2-mediated D prostanoid receptor 1 activation

journal articl

Cloud-Based Experimental Platform for the Space-Ground Integrated Network

The space-ground integrated network (SGIN) is an important direction of future network development and is expected to play an important role in edge computing for the Internet of Things (IoT). Through integration with an SGIN, IoT applications can provide services with long-distance and wide-coverage features. However, SGINs are typical large-scale and time-varying networks for which new network technologies, protocols, and applications must be rigorously evaluated and validated. Therefore, a reliable experimental platform is necessary for SGINs. This paper presents a cloud-based experimental platform for the SGIN context named SGIN-Stack. First, the architecture of SGIN-Stack, which combines the Systems Tool Kit (STK) and OpenStack, is described. Based on this architecture, a seamless linkage between OpenStack and STK is achieved to realize synchronous, dynamic, and real-time network emulation for an SGIN, and the dynamic differential compensation technology and a random number generation algorithm are applied to improve the emulation accuracy for satellite links. Finally, an emulation scenario is constructed that includes six space-based backbone nodes, sixty-six space-based access nodes, and a ground station. Based on this emulation scenario, experiments concerning the satellite link delays, bit error ratio (BER), and throughput are carried out to prove the high fidelity of our SGIN-Stack platform. Emulation experiments involving satellite orbital maneuvers and attitude adjustments show that SGIN-Stack can be used for dynamic and real-time SGIN emulation.</jats:p

A Facial Feature and Lip Movement Enhanced Audio-Visual Speech Separation Model

The cocktail party problem can be more effectively addressed by leveraging the speaker&rsquo;s visual and audio information. This paper proposes a method to improve the audio&rsquo;s separation using two visual cues: facial features and lip movement. Firstly, residual connections are introduced in the audio separation module to extract detailed features. Secondly, considering the video stream contains information other than the face, which has a minimal correlation with the audio, an attention mechanism is employed in the face module to focus on crucial information. Then, the loss function considers the audio-visual similarity to take advantage of the relationship between audio and visual completely. Experimental results on the public VoxCeleb2 dataset show that the proposed model significantly enhanced SDR, PSEQ, and STOI, especially 4 dB improvements in SDR

Antioxidant enzymes regulate reactive oxygen species during pod elongation in Pisum sativum and Brassica chinensis.

Previous research has focused on the involvement of reactive oxygen species (ROS) in cell wall loosening and cell extension in plant vegetative growth, but few studies have investigated ROS functions specifically in plant reproductive organs. In this study, ROS levels and antioxidant enzyme activities were assessed in Pisum sativum and Brassica chinensis pods at five developmental stages. In juvenile pods, the high levels of O2.- and .OH indicates that they had functions in cell wall loosening and cell elongation. In later developmental stages, high levels of .OH were also related to increases in cell wall thickness in lignified tissues. Throughout pod development, most of the O2.- was detected on plasma membranes of parenchyma cells and outer epidermis cells of the mesocarp, while most of the H2O2 was detected on plasma membranes of most cells throughout the mesocarp. This suggests that these sites are presumably the locations of ROS generation. The antioxidant enzymes superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) apparently contributed to ROS accumulation in pod wall tissues. Furthermore, specifically SOD and POD were found to be associated with pod growth through the regulation of ROS generation and transformation. Throughout pod development, O2.- decreases were associated with increased SOD activity, while changes in H2O2 accumulation were associated with changes in CAT and POD activities. Additionally, high POD activity may contribute to the generation of(.)OH in the early development of pods. It is concluded that the ROS are produced in different sites of plasma membranes with the regulation of antioxidant enzymes, and that substantial ROS generation and accumulation are evident in cell elongation and cell wall loosening in pod wall cells