796 research outputs found
Effect of organic acids on the growth and lipid accumulation of oleaginous yeast Trichosporon fermentans
<p>Abstract</p> <p>Background</p> <p>Microbial lipids have drawn increasing attention in recent years as promising raw materials for biodiesel production, and the use of lignocellulosic hydrolysates as carbon sources seems to be a feasible strategy for cost-effective lipid fermentation with oleaginous microorganisms on a large scale. During the hydrolysis of lignocellulosic materials with dilute acid, however, various kinds of inhibitors, especially large amounts of organic acids, will be produced, which substantially decrease the fermentability of lignocellulosic hydrolysates. To overcome the inhibitory effects of organic acids, it is critical to understand their impact on the growth and lipid accumulation of oleaginous microorganisms.</p> <p>Results</p> <p>In our present work, we investigated for the first time the effect of ten representative organic acids in lignocellulosic hydrolysates on the growth and lipid accumulation of oleaginous yeast <it>Trichosporon fermentans </it>cells. In contrast to previous reports, we found that the toxicity of the organic acids to the cells was not directly related to their hydrophobicity. It is worth noting that most organic acids tested were less toxic than aldehydes to the cells, and some could even stimulate the growth and lipid accumulation at a low concentration. Unlike aldehydes, most binary combinations of organic acids exerted no synergistic inhibitory effects on lipid production. The presence of organic acids decelerated the consumption of glucose, whereas it influenced the utilization of xylose in a different and complicated way. In addition, all the organic acids tested, except furoic acid, inhibited the malic activity of <it>T. fermentans</it>. Furthermore, the inhibition of organic acids on cell growth was dependent more on inoculum size, temperature and initial pH than on lipid content.</p> <p>Conclusions</p> <p>This work provides some meaningful information about the effect of organic acid in lignocellulosic hydrolysates on the lipid production of oleaginous yeast, which is helpful for optimization of biomass hydrolysis processes, detoxified pretreatment of hydrolysates and lipid production using lignocellulosic materials.</p
A thermodynamically consistent quasi-particle model without density-dependent infinity of the vacuum zero point energy
In this paper, we generalize the improved quasi-particle model proposed in J.
Cao et al., [ Phys. Lett. B {\bf711}, 65 (2012)] from finite temperature and
zero chemical potential to the case of finite chemical potential and zero
temperature, and calculate the equation of state (EOS) for (2+1) flavor Quantum
Chromodynamics (QCD) at zero temperature and high density. We first calculate
the partition function at finite temperature and chemical potential, then go to
the limit and obtain the equation of state (EOS) for cold and dense QCD,
which is important for the study of neutron stars. Furthermore, we use this EOS
to calculate the quark-number density, the energy density, the quark-number
susceptibility and the speed of sound at zero temperature and finite chemical
potential and compare our results with the corresponding ones in the existing
literature
Geometric effects of a quarter of corrugated torus
In the spirit of the thin-layer quantization scheme, we give the effective
Shr\"{o}dinger equation for a particle confined to a corrugated torus, in which
the geometric potential is substantially changed by corrugation. We find the
attractive wells reconstructed by the corrugation not being at identical
depths, which is strikingly different from that of a corrugated nanotube,
especially in the inner side of the torus. By numerically calculating the
transmission probability, we find that the resonant tunneling peaks and the
transmission gaps are merged and broadened by the corrugation of the inner side
of torus. These results show that the quarter corrugated torus can be used not
only to connect two tubes with different radiuses in different directions, but
also to filter the particles with particular incident~energies.Comment: 7 pages, 8 figure
Cold hardiness of Phauda flammans (Lepidoptera: Zygaenidae) larvae
This study aimed to determine the cold hardiness of Phauda flammans (Lepidoptera: Zygaenidae) larvae. Supercooling points of the 1st–6th instar larvae of P. flammans ranged from –7.7 to –13.0 °C. The lethal temperatures were –8 °C for 1st, –5 °C for 2nd, and –7 °C for 3rd–6th instars. Lethal times at the instar-specific lethal temperatures were 12 h for 1st, 14 h for 2nd, 15 h for 3rd, 17 h for 4th, and 18 h for 5th–6th instars. The times required for all larvae to die in an incubator at 5 °C were 30 d for 1st, 3rd, 4th, and 5th instars, and 25 d for 2nd and 6th instars. The findings suggest that P. flammans is a chill-intolerant species, and larvae will die if the air temperature decreases to –5 to –8 °C for 12–18 h or to 5 °C for 25–30 d. Such conditions are, however, unlikely to occur in southern China
- …