Evaluating the Risk of Tumors Diseases Based on Measurement of Urinary and Serumal Antioxidants Using the New Agar Diffusion Methods

Objectives. To discuss the characteristics of the amount of urinary total antioxidants in tumor diseases and the possibility of utilizing the changing regulation of urinary antioxidants to diagnose tumor diseases. Method. Urine and serum specimens from 130 healthy people were used to investigate the variation of antioxidant capacity against age. Urine and serum specimens from 44 unselected patients with tumors and 44 healthy people with same age background were used to explore the significance of urinary antioxidant capacity in clinic to diagnose tumor diseases. Potassium permanganate agar method and iodine starch method were used to determine the amount of total antioxidants. Results. In healthy people, more antioxidants in urine were measured in older people, while the results were opposite in serum. More antioxidants were found in urine of tumor patients than in healthy people with same age-range. Conclusions. According to the results of 130 measurements, the amount of antioxidants in urine varies by age. By using agar methods to measure antioxidants, the effect of age is required to be considered. Antioxidants levels from tumor patients were significantly higher than healthy individuals in urine. The combination of urine and serum to determine total antioxidants can better diagnose tumor diseases based on iodine starch method, with area under the receiver operating characteristics curve at 0.787

Influence of Croton tiglium seeds on the pharmacokinetics of anthraquinones in Radix et Rhizoma Rhei

In this study, the influence of Croton tiglium seeds on the pharmacokinetic of rhein in Radix et Rhizoma Rhei was performed to elucidate the compatibility relationship of these two herbs. The pharmacokinetics of rhein was studied following oral administration of pure rhein, Radix et Rhizoma Rhei methanol extract (RE) and Radix et Rhizoma Rhei and seeds of Croton tiglium mathanol extract (RCE), with approximately the same dose of 10mg/kg. It was found that comparing with that in monomer, both AUC and Cmax of rhein in RE and different proportion of RCE were significantly different. The results indicated that comparing with the monomer, the concentration of rhein in the plasma was increased in the group of RE whereas the tendency of AUC and Cmax of rhein in RCE presented quite discrepancy depending on the different proportion of RCE.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Room-Temperature Electrochemical Reduction of Epitaxial Bi₂O₃ Films to Epitaxial Bi Films

This work reports a new facile approach to fabricate high-quality epitaxial Bi thin films by direct electrochemical reduction of epitaxial δ-Bi2O3 thin films on Au single crystals in aqueous solution at room-temperature. The as-produced Bi thin films (without any post-annealing process) exhibit large grain sizes, continuous microstructures, and enhanced magnetotransport properties

The radiosensitization effects of Endostar on human lung squamous cancer cells H-520

<p>Abstract</p> <p>Background</p> <p>The present study mainly aimed to investigate the direct effects of Endostar (ES) on the proliferation and radiosensitivity of human lung squamous cancer cell line H-520.</p> <p>Results</p> <p>ES significantly inhibited H-520 cell proliferation in a time- and dose-dependent manner. According to the colony-forming assays, ES could increase the H-520 cell radiosensitivity. ES induced cell apoptosis, the apoptosis rate increased with the raise of ES concentration. Irradiation induced significantly higher apoptosis rate in ES-treated H-520 cells than non-treated H-520 cells. ES induced cell cycle distribution and G₀/G₁arrest in H-520 cells, whereas irradiation induced G₂/M arrest. The phospho-p38-MAPK and p-Akt protein levels were decreased in H-520 cells after ES treatment. Furthermore, activated caspase protein level increased and Bcl-2 protein levels decreased after treatment with ES and irradiation.</p> <p>Conclusion</p> <p>ES significantly enhanced the sensitivity of H-520 cells to irradiation by inhibition of cellular proliferation, promotion of cell apoptosis and redistribution of cell cycle, possibly via deactivation of Akt pathway. The present study supports the possibility to use the combination of ES and ionizing irradiation to treat patients with lung squamous cell cancer in clinics.</p