Object Contour and Edge Detection with RefineContourNet

A ResNet-based multi-path refinement CNN is used for object contour detection. For this task, we prioritise the effective utilization of the high-level abstraction capability of a ResNet, which leads to state-of-the-art results for edge detection. Keeping our focus in mind, we fuse the high, mid and low-level features in that specific order, which differs from many other approaches. It uses the tensor with the highest-levelled features as the starting point to combine it layer-by-layer with features of a lower abstraction level until it reaches the lowest level. We train this network on a modified PASCAL VOC 2012 dataset for object contour detection and evaluate on a refined PASCAL-val dataset reaching an excellent performance and an Optimal Dataset Scale (ODS) of 0.752. Furthermore, by fine-training on the BSDS500 dataset we reach state-of-the-art results for edge-detection with an ODS of 0.824.Comment: Keywords: Object Contour Detection, Edge Detection, Multi-Path Refinement CN

Scalar Perturbations Through Cycles

We analytically and numerically investigate the evolutions of the scalar perturbations through the cycles with nonsingular bounce. It is found that the amplitude of the curvature perturbation on large scale will be amplified cycle by cycle, and the isocurvature perturbations also obtain an amplification, but the rate of its amplification is slower than that of curvature perturbation, unless its coupling to the metric perturbation is not negligible.Comment: 7 pages, 10 figure

Hybrid quantum device based on NV centers in diamond nanomechanical resonators plus superconducting waveguide cavities

We propose and analyze a hybrid device by integrating a microscale diamond beam with a single built-in nitrogen-vacancy (NV) center spin to a superconducting coplanar waveguide (CPW) cavity. We find that under an ac electric field the quantized motion of the diamond beam can strongly couple to the single cavity photons via dielectric interaction. Together with the strong spin-motion interaction via a large magnetic field gradient, it provides a hybrid quantum device where the dia- mond resonator can strongly couple both to the single microwave cavity photons and to the single NV center spin. This enables coherent information transfer and effective coupling between the NV spin and the CPW cavity via mechanically dark polaritons. This hybrid spin-electromechanical de- vice, with tunable couplings by external fields, offers a realistic platform for implementing quantum information with single NV spins, diamond mechanical resonators, and single microwave photons.Comment: Accepted by Phys. Rev. Applie

Modelling of Capillary Pore Structure Evolution in Portland Cement Pastes Based on Irregular-Shaped Cement Particles

The pore structure plays a crucial role in durability performance of cement-based materials. However, the pore structure in cement pastes is highly dependent on the initial packing of cement particles and cement hydration process, which seems to be related to the shapes of cement particles. This paper proposed a numerical method to investigate the effect of cement particle shapes on capillary pore structures in cement pastes. In this study, irregular-shaped cement particles with various shapes are generated using a novel central growth model, and then incorporated into CEMHYD3D model to simulate Portland cement hydration. Some home-made programs of determining pore structure parameters including porosity, pore size distribution, connectivity and tortuosity are subsequently performed on the extracted three-dimensional network of capillary pore structure in cement pastes. The modelling results indicate that shape-induced large surface area in more non-equiaxed irregular-shaped cement particles can improve pore structure parameters in hardened cement pastes, but this effect will be slight in the later curing period and at a low water-tocement ratio. In addition, the less considered geometric difference plays a role in pore structure evolution especially for extremely non-equiaxed cement particle. However, the geometric attribute has a weak effect on pore structure parameters overall. The modelling results can provide a new insight into durability design in cement-based materials by means of manipulating cement particle shape in the future

The effects of disorder and interactions on the Anderson transition in doped Graphene

We undertake an exact numerical study of the effects of disorder on the Anderson localization of electronic states in graphene. Analyzing the scaling behaviors of inverse participation ratio and geometrically averaged density of states, we find that Anderson metal-insulator transition can be introduced by the presence of quenched random disorder. In contrast with the conventional picture of localization, four mobility edges can be observed for the honeycomb lattice with specific disorder strength and impurity concentration. Considering the screening effects of interactions on disorder potentials, the experimental findings of the scale enlarges of puddles can be explained by reviewing the effects of both interactions and disorder.Comment: 7 pages, 7 figure

Activation and degranulation of CAR-T cells using engineered antigen-presenting cell surfaces

Adoptive cell transfer of Chimeric Antigen Receptor (CAR)-T cells showed promising results in patients with B cell malignancies. However, the detailed mechanism of CAR-T cell interaction with the target tumor cells is still not well understood. This work provides a systematic method for analyzing the activation and degranulation of second-generation CAR-T cells utilizing antigen-presenting cell surfaces. Antigen-presenting cell surfaces composed of circular micropatterns of CAR-specific anti-idiotype antibodies have been developed to mimic the interaction of CAR-T cells with target tumor cells using micro-contact printing. The levels of activation and degranulation of fixed non-transduced T cells (NT), CD19.CAR-T cells, and GD2.CAR-T cells on the antigen-presenting cell surfaces were quantified and compared by measuring the intensity of the CD3ζ chain phosphorylation and the Lysosome-Associated Membrane Protein 1 (LAMP-1), respectively. The size and morphology of the cells were also measured. The intracellular Ca2+ flux of NT and CAR-T cells upon engagement with the antigen-presenting cell surface was reported. Results suggest that NT and CD19.CART cells have comparable activation levels, while NT have higher degranulation levels than CD19.CAR-T cells and GD2.CAR-T cells. The findings show that antigen-presenting cell surfaces allow a quantitative analysis of the molecules involved in synapse formation in different CAR-T cells in a systematic, reproducible manner

The impact of hospital attributes on patient choice for first visit

The underutilization of primary care in urban China threatens the efficiency and effectiveness of the Chinese health system. To guide patient flow to primary care, the Chinese government has rolled out a sequence of health care reforms which improve the affordability, the infrastructure and workforce of the primary care system. However, these measures have not yielded the desired effect on the utilization of primary care, which is lowest in urban areas. It is unclear how the factors identified to influence facility choice in urban China are actually impacting choice behaviour. We conducted a discrete choice experiment to elicit the quantitative impact of facility attributes when choosing a health care facility for first visit and analysed how the stated choice varies with these attributes. We found that the respondents placed different weights on the identified attributes, depending on whether they perceived their condition to be minor or severe. For conditions perceived as minor, the respondents valued visit time, equipment and medical skill most. For conditions perceived as severe, they placed most importance on equipment, travel time and facility size. We found that for conditions perceived as minor, only 14% preferred visiting a facility over opting out, a percentage which would more than double to 37% if community health centres were maximally improved. For conditions perceived as severe, improvements in community health centres may almost double first visits to primary care, mostly from patients who would otherwise choose higher-level facilities. Our findings suggest that for both severity conditions, improvements to medical equipment and medical skill at community health centres in urban China can effectively direct patient flow to primary care and promote the efficiency and effectiveness of the urban health system

Radiation-induced Assembly of Rad51 and Rad52 Recombination Complex Requires ATM and c-Abl

Cells from individuals with the recessive cancer-prone disorder ataxia telangiectasia (A-T) are hypersensitive to ionizing radiation (I-R). ATM (mutated in A-T) is a protein kinase whose activity is stimulated by I-R. c-Abl, a nonreceptor tyrosine kinase, interacts with ATM and is activated by ATM following I-R. Rad51 is a homologue of bacterial RecA protein required for DNA recombination and repair. Here we demonstrate that there is an I-R-induced Rad51 tyrosine phosphorylation, and this induction is dependent on both ATM and c-Abl. ATM, c-Abl, and Rad51 can be co-immunoprecipitated from cell extracts. Consistent with the physical interaction, c-Abl phosphorylates Rad51 in vitro and in vivo. In assays using purified components, phosphorylation of Rad51 by c-Abl enhances complex formation between Rad51 and Rad52, which cooperates with Rad51 in recombination and repair. After I-R, an increase in association between Rad51 and Rad52 occurs in wild-type cells but not in cells with mutations that compromise ATM or c-Abl. Our data suggest signaling mediated through ATM, and c-Abl is required for the correct post-translational modification of Rad51, which is critical for the assembly of Rad51 repair protein complex following I-R