Wind loads on multiple cylinders arranged in tandem with effects of turbulence and surface roughness

The goal of this study is to investigate the characteristics of mean drag and lift forces for a series of cylinders arranged in tandem with varying flow turbulence and surface roughness. This goal was fulfilled by building a wind tunnel test section capable of generating both smooth flow and turbulent flow, and by adopting cylinder models with certain types of surface roughness as well as the smooth surface. In both smooth flow and grid-turbulence flow conditions, single cylinders as well as multiple cylinders were tested. The tested Reynolds number ranged from 2.7×104 to 8.6×104. For multiple-cylinder tests, a majority of the tests were conducted with cylinders aligned in tandem or as tops-leveled for unequal diameter cases. A limited number of experiments were conducted for different alignment cases as the comparison. The mean drag and lift coefficients were reported for individual cylinders as well as for combinations. A significant effect of flow turbulence was observed on both drag coefficients and lift coefficients. The critical spacing was observed for most combinations in the smooth flow condition, which featured with an abrupt change of drag coefficients with a small change of spacing. However, this feature was much less pronounced when these combinations were tested in the turbulent flow. It was also found that for the combinations with larger cylinder in the upstream position, large combined drag coefficients and small lift coefficients were more likely to occur, especially in the smooth-flow conditions. The force ratios were reported for all the tested combinations, which measure the reduction of the combined drag force compared to the sum of the drag forces when the cylinders stand independently. Results of force ratios showed that there was more reduction with more cylinders in the tandem group, and the reduction became less when the spacing between the cylinders increased. Recommendations for the design practice were developed based on the force ratios obtained from the tests in the turbulent flow condition

Chlorine and Bromine Isotope Fractionation of Halogenated Organic Pollutants on Gas Chromatography Columns

Compound-specific chlorine/bromine isotope analysis (CSIA-Cl/Br) has become a useful approach for degradation pathway investigation and source appointment of halogenated organic pollutants (HOPs). CSIA-Cl/Br is usually conducted by gas chromatography-mass spectrometry (GC-MS), which could be negatively impacted by chlorine and bromine isotope fractionation of HOPs on GC columns. In this study, 31 organochlorines and 4 organobromines were systematically investigated in terms of Cl/Br isotope fractionation on GC columns using GC-double focus magnetic-sector high resolution MS (GC-DFS-HRMS). On-column chlorine/bromine isotope fractionation behaviors of the HOPs were explored, presenting various isotope fractionation modes and extents. Twenty-nine HOPs exhibited inverse isotope fractionation, and only polychlorinated biphenyl-138 (PCB-138) and PCB-153 presented normal isotope fractionation. And no observable isotope fractionation was found for the rest four HOPs, i.e., PCB-101, 1,2,3,7,8-pentachlorodibenzofuran, PCB-180 and 2,3,7,8-tetrachlorodibenzofuran. The isotope fractionation extents of different HOPs varied from below the observable threshold (0.50%) to 7.31% (PCB-18). The mechanisms of the on-column chlorine/bromine isotope fractionation were tentatively interpreted with the Craig-Gordon model and a modified two-film model. Inverse isotope effects and normal isotope effects might contribute to the total isotope effects together and thus determine the isotope fractionation directions and extents. Proposals derived from the main results of this study for CSIA-Cl/Br research were provided for improving the precision and accuracy of CSIA-Cl/Br results. The findings of this study will shed light on the development of CSIA-Cl/Br methods using GC-MS techniques, and help to implement the research using CSIA-Cl/Br to investigate the environmental behaviors and pollution sources of HOPs.Comment: 30 pages, 5 figure

Are You A Risk Taker? Adversarial Learning of Asymmetric Cross-Domain Alignment for Risk Tolerance Prediction

Most current studies on survey analysis and risk tolerance modelling lack professional knowledge and domain-specific models. Given the effectiveness of generative adversarial learning in cross-domain information, we design an Asymmetric cross-Domain Generative Adversarial Network (ADGAN) for domain scale inequality. ADGAN utilizes the information-sufficient domain to provide extra information to improve the representation learning on the information-insufficient domain via domain alignment. We provide data analysis and user model on two data sources: Consumer Consumption Information and Survey Information. We further test ADGAN on a real-world dataset with view embedding structures and show ADGAN can better deal with the class imbalance and unqualified data space than state-of-the-art, demonstrating the effectiveness of leveraging asymmetrical domain information

cRVR: A Stackelberg Game Approach for Joint Privacy-Aware Video Requesting and Edge Caching

As users conveniently stream their favored online videos, video request records will be automatically seized by video content providers, which may leak users' privacy. Unfortunately, most existing privacy-enhancing approaches are not applicable for protecting users' privacy in requests, which cannot be easily altered or distorted by users and must be visible for content providers to stream correct videos. To preserve request privacy in online video services, it is possible to request additional videos irrelevant to users' interests so that content providers cannot precisely infer users' interest information. However, a naive redundant requesting approach will significantly degrade the performance of edge caches and increase bandwidth overhead accordingly. In this paper, we are among the first to propose a Cache-Friendly Redundant Video Requesting (cRVR) algorithm for User Devices (UDs) and its corresponding caching algorithm for the Edge Cache (EC), which can effectively mitigate the problem of request privacy leakage with minimal impact on the EC's performance. To solve the problem, we develop a Stackelberg game to analyze the dedicated interaction between UDs and EC and obtain their optimal strategies to maximize their respective utility. For UDs, the utility function is a combination of both video playback utility and privacy protection utility. We theoretically prove the existence and uniqueness of the equilibrium of the Stackelberg game. In the end, extensive experiments are conducted with real traces to demonstrate that cRVR can effectively protect video request privacy by reducing up to 57.96\% of privacy disclosure compared to baseline algorithms. Meanwhile, the caching performance of ECs is only slightly affected

The new oncogene transmembrane protein 60 is a potential therapeutic target in glioma

Glioma is a malignant tumor with a high fatality rate, originating in the central nervous system. Even after standard treatment, the prognosis remains unsatisfactory, probably due to the lack of effective therapeutic targets. The family of transmembrane proteins (TMEM) is a large family of genes that encode proteins closely related to the malicious behavior of tumors. Thus, it is necessary to explore the molecular and clinical characteristics of newly identified oncogenes, such as transmembrane protein 60 (TMEM60), to develop effective treating options for glioma. We used bioinformatic methods and basic experiments to verify the expression of transmembrane protein 60 in gliomas and its relationship with 1p and 19q (1p19q) status, isocitrate dehydrogenase (IDH) status, patient prognosis, and immune cell infiltration using public databases and clinical samples. In addition, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to detect co-expressed genes. Thus, we inhibited the expression of transmembrane protein 60 to observe the proliferation and activity of glioma LN229 cells. We found transmembrane protein 60 was significantly upregulated in glioma compared with that in normal brain tissue at the mRNA. In the subgroups of World Health Organization high grade, isocitrate dehydrogenase wildtype, 1p and 19q non-codeletion, or isocitrate dehydrogenase wild combined with 1p and 19q non-codeletion, the expression of transmembrane protein 60 increased, and the prognosis of glioma patients worsened. In the transmembrane protein 60 high expression group, infiltration of immune cells and stromal cells in the tumor microenvironment increased, tumor purity decreased, and immune cells and pathways were activated. The immune cells mainly included regulatory T-cell, gamma delta T-cell, macrophages M0, neutrophils, and CD8+ T-cells. Overexpression of co-inhibitory receptors (CTLA4, PDL1 and CD96) may promote the increase of depletion of T-cell, thus losing the anti-tumor function in the transmembrane protein 60 high expression group. Finally, we found that transmembrane protein 60 silencing weakened the viability, proliferation, and colony formation of glioma LN229 cells. This is the 0 report on the abnormally high expression of transmembrane protein 60 in glioma and its related clinical features, such as tumor microenvironment, immune response, tumor heterogeneity, and patient prognosis. We also found that transmembrane protein 60 silencing weakened the proliferation and colony formation of glioma LN229 cells. Thus, the new oncogene transmembrane protein 60 might be an effective therapeutic target for the clinical treatment of glioma

Analyzing the Effectiveness of Policy Instruments on New Energy Vehicle Industry using Consistent Fuzzy Preference Relations A case study in China

New energy vehicles (NEVs) have important implications for traffic pollution management and energy consumption. A series of industrial incentive policies have been introduced to promote the development of the NEV industry. However, the actual effects of the policies do not come up to expectations. Aiming to analyze the relationship between the policy instruments and the market acceptance of NEVs, a consistent fuzzy preference relations model is proposed to evaluate the efficiency of policies on the NEV industry. The model introduces an assessment criteria system with three criterions (industry development, technology research and development (R&D), and NEV popularization). The quantitative weights of evaluation criteria are calculated by the fuzzy preference relation. The weights indicate the significance of the factors in the development of NEVs, especially when NEVs have not been widely accepted. With the proposed model, a case study in China shows that the policy of technology R&D is the most crucial for the NEV industry in China. The policy of NEV industry development, on the other hand, is equally important. The results are hoped to give a better understanding of the relationship between government policy instruments and NEV development, the measures for enhancing the policy efficiency of NEV development

Nutlin-3 overcomes arsenic trioxide resistance and tumor metastasis mediated by mutant p53 in Hepatocellular Carcinoma

Background: Arsenic trioxide has been demonstrated as an effective anti-cancer drug against leukemia and solid tumors both in vitro and in vivo. However, recent phase II trials demonstrated that single agent arsenic trioxide was poorly effective against hepatocellular carcinoma (HCC), which might be due to drug resistance. Methods: Mutation detection of p53 gene in arsenic trioxide resistant HCC cell lines was performed. The therapeutic effects of arsenic trioxide and Nutlin-3 on HCC were evaluated both in vitro and in vivo. A series of experiments including MTT, apoptosis assays, co-Immunoprecipitation, siRNA transfection, lentiviral infection, cell migration, invasion, and epithelial-mesenchy-mal transition (EMT) assays were performed to investigate the underlying mechanisms. Results: The acquisition of p53 mutation contributed to arsenic trioxide resistance and enhanced metastatic potential of HCC cells. Mutant p53 (Mutp53) silence could re-sensitize HCC resistant cells to arsenic trioxide and inhibit the metastatic activities, while mutp53 overexpression showed the opposite effects. Neither arsenic trioxide nor Nutlin-3 could exhibit obvious effects against arsenic trioxide resistant HCC cells, while combination of them showed significant effects. Nutlin-3 can not only increase the intracellular arsenicals through inhibition of p-gp but also promote the p73 activation and mutp53 degradation mediated by arsenic trioxide. In vivo experiments indicated that Nutlin-3 can potentiate the antitumor activities of arsenic trioxide in an orthotopic hepatic tumor model and inhibit the metastasis to lung. Conclusions: Acquisitions of p53 mutations contributed to the resistance of HCC to arsenic trioxide. Nutlin-3 could overcome arsenic trioxide resistance and inhibit tumor metastasis through p73 activation and promoting mutant p53 degradation mediated by arsenic trioxide