Emergence of Extensively Drug-Resistant Proteus mirabilis Harboring a Conjugative NDM-1 Plasmid and a Novel Salmonella Genomic Island 1 Variant, SGI1-Z

Acquisition of blaNDM-1 in bacterial species, such as Proteus mirabilis that is intrinsically resistant to tetracycline, tigecycline and colistin, will make clinical treatment extremely difficult. Here, we characterized an NDM-1-producing clinical isolate of P. mirabilis (PM58) that displayed an extensively drug-resistant (XDR) phenotype, susceptible only to aztreonam. Molecular analysis revealed that PM58 harbored both a conjugative NDM-1 plasmid and a novel Salmonella genomic island 1 variant on chromosome

High Incidence and Endemic Spread of NDM-1-Positive Enterobacteriaceae in Henan Province, China

The emergence and spread of New Delhi metallo-β-lactamase 1 (NDM-1)-producing carbapenem-resistant Enterobacteriaceae (CRE) present an urgent threat to human health. In China, the blaNDM-1 gene has been reported mostly in Acinetobacter spp. but is rarely found in Enterobacteriaceae. Here, we report a high incidence and endemic spread of NDM-1-producing CRE in Henan Province in China. Sixteen (33.3%) of the 48 CRE isolates obtained from patients during June 2011 to July 2012 were positive for blaNDM-1, and the gene was found to be carried on plasmids of various sizes (∼55 to ∼360 kb). These plasmids were readily transferrable to recipient Escherichia coli by conjugation, conferred resistance to multiple antibiotics, and belonged to multiple replicon types. The blaNDM-1-positive CRE isolates were genetically diverse, and six new multilocus sequence typing (MLST) sequence types were linked to the carriage of NDM-1. Five of the isolates were classified as extensively drug-resistant (XDR) isolates, four of which also carried the fosA3 gene conferring resistance to fosfomycin, an alternative drug for treating infections by CRE. In each blaNDM-1-positive CRE isolate, the blaNDM-1 gene was downstream of an intact ISAba125 element and upstream of the bleMBL gene. Furthermore, gene environment analysis suggested the possible transmission of blaNDM-1-containing sequences from Acinetobacter spp. to Klebsiella pneumoniae and Klebsiella oxytoca. These findings reveal the emergence and active transmission of NDM-1-positive CRE in China and underscore the need for heightened measures to control their further spread

Whole-genome resequencing of 472 Vitis accessions for grapevine diversity and demographic history analyses

Despite the importance of grapevine cultivation in human history and the economic values of cultivar improvement, large-scale genomic variation data are lacking. Here the authors resequence 472 Vitis accessions and use the identified genetic variations for domestication history, demography, and GWAS analyses

Potential Effects of Urbanization on Precipitation Extremes in the Pearl River Delta Region, China

Rapid urbanization plays an indelible role in modifying local climate, with more extreme precipitation in urban areas. Understanding the mechanism of urban-induced precipitation changes and quantifying the potential effects of urbanization on the changes in precipitation extremes have become hotspot issues in hydrometeorology. We examine the spatiotemporal changes of precipitation extremes over the Pearl River Delta region in China using the homogenized daily precipitation dataset from the period 1961–2017, and quantify the urbanization effects on these changes. Most of the extreme precipitation indices show increasing trends, but only the mean precipitation intensity has a significant increase. Urbanization could induce the intensification of extreme precipitation, with a higher amount, intensity, and frequency of precipitation extremes and a larger magnitude of their trends in urban areas by comparison with those rural areas. Moreover, high-level urbanization tends to make a greater contribution to the temporal changes in precipitation extremes, indicating that urbanization effects on precipitation extremes may be related to urbanization levels. However, urbanization level shows little effect on the changes in the spatial patterns of precipitation extremes, with similar spatial distribution in different urbanization stages. Our findings highlight the important role of urbanization in precipitation extremes and offer insights into the feedback of anthropogenic changes into variations in precipitation extremes

Potential Effects of Urbanization on Precipitation Extremes in the Pearl River Delta Region, China

Rapid urbanization plays an indelible role in modifying local climate, with more extreme precipitation in urban areas. Understanding the mechanism of urban-induced precipitation changes and quantifying the potential effects of urbanization on the changes in precipitation extremes have become hotspot issues in hydrometeorology. We examine the spatiotemporal changes of precipitation extremes over the Pearl River Delta region in China using the homogenized daily precipitation dataset from the period 1961–2017, and quantify the urbanization effects on these changes. Most of the extreme precipitation indices show increasing trends, but only the mean precipitation intensity has a significant increase. Urbanization could induce the intensification of extreme precipitation, with a higher amount, intensity, and frequency of precipitation extremes and a larger magnitude of their trends in urban areas by comparison with those rural areas. Moreover, high-level urbanization tends to make a greater contribution to the temporal changes in precipitation extremes, indicating that urbanization effects on precipitation extremes may be related to urbanization levels. However, urbanization level shows little effect on the changes in the spatial patterns of precipitation extremes, with similar spatial distribution in different urbanization stages. Our findings highlight the important role of urbanization in precipitation extremes and offer insights into the feedback of anthropogenic changes into variations in precipitation extremes

Remote Sensing Scene Data Generation Using Element Geometric Transformation and GAN-Based Texture Synthesis

Classification of remote sensing scene image (RSSI) has been broadly applied and has attracted increasing attention. However, scene classification methods based on convolutional neural networks (CNNs) require a large number of manually labeled samples as training data, which is time-consuming and costly. Therefore, generating labeled data becomes a practical approach. However, conventional scene generation based on generative adversarial networks (GANs) involve some significant limitations, such as distortion and limited size. To solve the mentioned problems, herein, we propose a method of RSSI generation using element geometric transformation and GAN-based texture synthesis. Firstly, we segment the RSSI, extracting the element information in the RSSI. Then, we perform geometric transformations on the elements and extract the texture information in them. After that, we use the GAN-based method to model and generate the texture. Finally, we fuse the transformed elements with the generated texture to obtain the generated RSSI. The geometric transformation increases the complexity of the scene. The GAN-based texture synthesis ensures the generated scene image is not distorted. Experimental results demonstrate that the RSSI generated by our method achieved a better visual effect than a GAN model. In addition, the performance of CNN classifiers was reduced by 0.44–3.41% on the enhanced data set, which is partly attributed to the complexity of the generated samples. The proposed method was able to generate diverse scene data with sufficient fidelity under conditions of small sample size and solve the accuracy saturation issues of the public scene data sets

CRISPR/Cas9-mediated efficient targeted mutagenesis in Chardonnay (Vitis vinifera L.)

The type II clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 system (CRISPR/Cas9) has been successfully applied to edit target genes in multiple plant species. However, it remains unknown whether this system can be used for genome editing in grape. In this study, we described genome editing and targeted gene mutation in 'Chardonnay' suspension cells and plants via the CRISPR/Cas9 system. Two single guide RNAs (sgRNAs) were designed to target distinct sites of the L-idonate dehydrogenase gene (IdnDH). CEL I endonuclease assay and sequencing results revealed the expected indel mutations at the target site, and a mutation frequency of 100% was observed in the transgenic cell mass (CM) as well as corresponding regenerated plants with expression of sgRNA1/Cas9. The majority of the detected mutations in transgenic CM were 1-bp insertions, followed by 1-to 3-nucleotide deletions. Off-target activities were also evaluated by sequencing the potential off-target sites, and no obvious off-target events were detected. Our results demonstrated that the CRISPR/Cas9 system is an efficient and specific tool for precise genome editing in grape