COMPUTER SIMULATION OF THE PESTICIDE DEPOSITION DISTRIBUTION IN HORIZONTAL DIRECTION SPRAY

Abstract: The objective of this study is taken to realize pesticide precision spray of fruit trees and the other crops and reduce the deposition losses outside the canopy when the real time sensing technology was used in the pesticide target spray. In this paper the Pesticide solution deposition distribution experiments were conducted with two different volume median diameter (VMD) hollow cone nozzles fixed in horizontal direction, to investigate the influence of spray pressure and spray ground speed on the spray deposition region. The probability distribution model of the pesticide deposition was constructed based on the experiments, and the pesticide spray distribution range was simulated by using Matlab statistic toolbox. The simulation result showed that the spray pressure and the ground speed had the great influence on the maximum spray distance. With the increase of the spray speed, the spray deposition distribution range decreases gradually, when the nozzle 200 is under the speed above 1.20km/h and nozzle 300 is under the speed above 2.22km/h, the deposition range was reduced greatly. So the computer simulations make a reference for the choice of the spray control parameters

Semiquantum key distribution using initial states in only one basis without the classical user measuring

From the perspective of resource theory, it is interesting to achieve the same quantum task using as few quantum resources as possible. Semiquantum key distribution (SQKD), which allows a quantum user to share a confidential key with a classical user who prepares and operates qubits in only one basis, is an important example for studying this issue. To further limit the quantum resources used by users, in this paper, we constructed the first SQKD protocol which restricts the quantum user to prepare quantum states in only one basis and removes the classical user's measurement capability. Furthermore, we prove that the constructed protocol is unconditionally secure by deriving a key rate expression of the error rate in the asymptotic scenario. The work of this paper provides inspiration for achieving quantum superiority with minimal quantum resources.Comment: 13 pages, 3 figure

Subcellular localization and expression analysis of the BmDSCLP protein from silkworm, Bombyx mori

Leucine-rich repeat (LRR) proteins play important roles in the transduction of cellular signals and activation of defense responses. By scanning the cDNA library of silkworm (Bombyx mori) pupae constructed in our laboratory, we identified a 1557 bp gene that encodes a protein homologous to the death-associated small cytoplasmic leucine-rich protein, which was named as BmDSCLP. The full-length gene (GenBank accession no. FJ602779) contained a 642 bp open reading frame (ORF) encoding 213 amino acid residues. The ORF of this gene was inserted into the prokaryotic expression vector pET-28a(+) to construct a recombinant expression plasmid and the fusion protein was expressed in Escherichia coli BL21(DE3) cells. The fusion protein was purified by Ni-affinity chromatography and fast protein liquid chromatography (FPLC) and its size was then, determined by liquid chromatography-mass spectrometry (LC/MS/MS) and found to be 27.74 kD. Polyclonal antibodies were raised by subcutaneous injection of the recombinant protein into New Zealand white rabbits and the titer reached 1:12800. Analysis of the subcellular localization of the BmDSCLP protein revealed that, the protein was localized in both the cytoplasm and nucleus, but the amount in the former was slightly higher than that in the latter. In addition, real-time fluorescence quantification polymerase chain reaction studies were conducted to investigate BmDSCLP transcription at different developmental stages and in different tissues of the fifth instar larva. The results indicated that, BmDSCLP is widely transcribed in different stages and tissues of the silkworm. Analysis of stage-specific transcription patterns indicated that, the transcriptional level of BmDSCLP was highest in adults and lowest in eggs. Analysis of tissue-specific transcription patterns revealed that, the transcriptional level of BmDSCLP was highest in genital organs and lowest in silk glands. These results suggest that BmDSCLP plays important roles in the reproductive development of B. mori.Keywords: Bombyx mori, death-associated small cytoplasmic leucine-rich protein, prokaryotic expression, fluorescence quantification polymerase chain reactio

Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori

The Drosophila E(spl)m4 gene contains some highly conserved motifs (such as the Brd box, GY box, K box, and CAAC motif) in its 3′ untranslated region (3′ UTR). It was shown to be a microRNA target gene in Drosophila and to play an important role in the regulation of neurogenesis. We identified a homologue of the E(spl)m4 gene from Bombyx mori called BmEm4 and examined the expression patterns of BmEm4 mRNA and protein. There was a lack of correlation in the expression of the mRNA and protein between the different developmental stages, which raises the possibility of posttranscriptional regulation of the BmEm4 mRNA. Consistent with this idea is the finding that the 3′ UTR contains two putative binding sites for microRNAs. Moreover, given that the expression is the highest in the larval head, as confirmed by immunohistochemistry, we propose that BmEm4 may also be involved in the regulation of neurogenesis. Immunostaining indicated that BmEm4 is located primarily in the cytoplasm

In Vivo Bioassay of Recombinant Human Growth Hormone Synthesized in B. mori Pupae

The human growth hormone (hGH) has been expressed in prokaryotic expression system with low bioactivity previously. Then the effective B. mori baculovirus system was employed to express hGH identical to mature hGH successfully in larvae, but the expression level was still limited. In this work, the hGH was expressed in B. mori pupae by baculovirus system. Quantification of recombinant hGH protein (BmrhGH) showed that the expression of BmrhGH reached the level of approximately 890 μg/mL pupae supernatant solution, which was five times more than the level using larvae. Furthermore, Animals were gavaged with BmrhGH at the dose of 4.5 mg/rat.day, and the body weight gain (BWG) of treated group had a significant difference (P < .01) compared with the control group. The other two parameters of liver weight and epiphyseal width were also found to be different between the two groups (P < .05). The results suggested that BmrhGH might be used as a protein drug by oral administration

“Reading A Book, By A Gain In Your Wit”--How to cultivate high school students' sustainable reading: A multi-factor analysis

Reading cultivation is an important task of education. In order to ensure the sustainable reading behavior of high school students, more and more scholars begin to pay attention to the research of reading psychology. In order to explore the psychological mechanism of high school students' reading, based on the theory of self-efficacy and achievement motivation, this study explores the chain intermediary model of 600 high school students' reading motivation influencing reading satisfaction through reading self-efficacy and social support. The results show that there are significant positive predictors among reading motivation, reading self-efficacy, social support and reading satisfaction. At the same time, reading self-efficacy and social support play an intermediary role between reading motivation and reading satisfaction of high school students respectively, and jointly play a chain intermediary role. The research results are of great theoretical and practical significance for understanding the reading psychology of senior high school students and ensuring their sustainable reading behavior

Design and Experimental Investigation of a Rotational Piezoelectric Energy Harvester with an Offset Distance from the Rotation Center

Rotational energy harvesting technology has attracted more and more attention recently. This paper presents a piezoelectric rotational energy harvester that can be mounted with an offset distance from the rotation center. The piezoelectric energy harvester is designed to be dynamically excited by the force due to gravity, which causes the piezoelectric cantilever beams in the harvester to vibrate periodically as the harvester rotates. A novel design of the harvester structure with a hollow mass is proposed and analyzed in this paper. Experiments were performed to investigate the design and analysis. A power output of 106~2308 &mu;W can be achieved at the rotating frequencies of 0.79~14 Hz with a piezoelectric cantilever beam in the prototyped energy harvester. Results showed that the prototyped harvester can be mounted on a rotating wheel hub and output sufficient power in a wide frequency range for wireless monitoring sensors

Ring-Overlap: A Storage Scaling Mechanism for Hyperledger Fabric

Currently, blockchain is facing a serious storage explosion problem. While most storage scaling schemes are focused on permissionless blockchain, we propose the ring-overlap mechanism for consortium blockchain and use it to scale Hyperledger Fabric. In our scheme, all accounting nodes are divided into clusters, and each cluster contains several nodes; then, a portion of the entire block data is stored in a cluster. Block data is stored overlappingly on some cluster nodes, and each block is guaranteed to have some copies in a cluster. Theoretical analysis and simulation show that the storage occupied by nodes is significantly reduced in blockchain applications with frequent transactions, and the mechanism can still guarantee data integrity in the case of partial node failures in a single cluster. Furthermore, for transaction-frequent applications, storage space consumption can be significantly reduced without increasing excessive query time overhead

Synthesis of a Novel Rigid Semi-Alicyclic Dianhydride and Its Copolymerized Transparent Polyimide Films’ Properties

A new series of colorless polyimides (CPIs) with outstanding thermal properties and mechanical properties were fabricated by the copolymerization of a novel dianhydride and 4,4′-(hexafluoroisopropylidene)diphthalic anhydride (6FDA) with 2,2′-bistrifluoromethyl benzidine (TFDB). The novel dianhydride, 10-oxo-9-phenyl-9-(trifluoromethyl)-9,10-dihydroanthracene-2,3,6,7-tetraacid dianhydride (3FPODA), possessed a rigid semi-alicyclic structure, –CF3 and phenyl side groups, and an active carbonyl group. Benefitting from the special structure of 3FPODA, the glass transition temperatures (Tg) of the new CPIs improved from 330 °C to 377 °C, the coefficient of thermal expansion (CTE) decreased from 46 ppm/K to 24 ppm/K, and the tensile strength (TS), tensile modulus (TM), and elongation at break (EB) increased from 84 MPa to 136 MPa, 3.2 GPa to 4.4 GPa, and 2.94% to 4.13% with the increasing amount of 3FPODA, respectively. Moreover, the active carbonyl group of the 3FPODA could enhance the CPI’s adhesive properties. These results render the new dianhydride 3FPODA an ideal candidate monomer for the fabrication of high-performance CPIs