4,441 research outputs found
Design of the LLRF control system for MA cavity at CSNS RCS
The China Spallation Neutron Source (CSNS) beam power was successfully
reached 125 kW with a low beam loss in February 2022. In order to increase beam
power, during the summer in 2022, we employ magnetic-alloy (MA) cavity in the
rapid cycling synchrotron (RCS). It is a wideband cavity (Q=2), allows the
second harmonic rf (h= 4) operation, with the existing ferrite cavity to
realize the dual-harmonic acceleration. The second harmonic (h=4) is used for
the bunch shape control and alleviating the space charge effects. We design of
the low-level RF(LLRF) control system for MA cavity, in this paper, we describe
the system design and implementation, and the preliminary test results.Comment: Poster presented at LLRF Workshop 2023 (LLRF2023, arXiv: 2310.03199
BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana
The unfolded protein response (UPR) is required for protein homeostasis in the endoplasmic reticulum (ER) when plants are challenged by adverse environmental conditions. Inositol-requiring enzyme 1 (IRE1), the bifunctional protein kinase / ribonuclease, is an important UPR regulator in plants mediating cytoplasmic splicing of the mRNA encoding the transcription factor bZIP60. This activates the UPR signaling pathway and regulates canonical UPR genes. However, how the protein activity of IRE1 is controlled during plant growth and development is largely unknown. In the present study, we demonstrate that the nuclear and Golgi-localized protein BLISTER (BLI) negatively controls the activity of IRE1A/IRE1B under normal growth condition in Arabidopsis. Loss-of-function mutation of BLI results in chronic up-regulation of a set of both canonical UPR genes and non-canonical UPR downstream genes, leading to cell death and growth retardation. Genetic analysis indicates that BLI-regulated vegetative growth phenotype is dependent on IRE1A/IRE1B but not their canonical splicing target bZIP60. Genetic complementation with mutation analysis suggests that the D570/K572 residues in the ATP-binding pocket and N780 residue in the RNase domain of IRE1A are required for the activation of canonical UPR gene expression, in contrast, the D570/K572 residues and D590 residue in the protein kinase domain of IRE1A are important for the induction of non-canonical UPR downstream genes in the BLI mutant background, which correlates with the shoot growth phenotype. Hence, our results reveal the important role of IRE1A in plant growth and development, and BLI negatively controls IRE1A’s function under normal growth condition in plants
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