Parthenolide attenuates LPS-induced activation of NF-κB in a time-dependent manner in rat myocardium.

Parthenolide (PTN), a selective nuclear factor kappa B (NF-κB) inhibitor, has been used extensively to inhibit NF-κB activation. The duration of the inhibitory effect of PTN on NF-κB in vivo remains unclear. This study was to determine whether a lipopolysaccharide (LPS) challenge 6, 12 and 24 h after the administration of PTN could activate NF-κB. Rats were devided into five groups. The rats in the PTN, PTN+LPS and DMSO groups were injected intraperitoneally with PTN or DMSO. After 6, 12 or 24 h, LPS was administered in LPS and PTN+LPS groups. The expressions of NF-κB p50, IκBα and p-IκBα were inhibited in both PTN and PTN+LPS group at end of 6 and 12 h and no effects at 24 h. In summary, myocardial NF-κB expression occurs 1 h after the administration of LPS. PTN blocks this effect given at 6 h and no inhibitory effect 24 h after administration in vivo

A proof of Mader's conjecture on large clique subdivisions in C4C_4-free graphs

Given any integers $s,t\geq 2$, we show there exists some $c=c(s,t)>0$ such that any $K_{s,t}$-free graph with average degree $d$ contains a subdivision of a clique with at least $cd^{\frac{1}{2}\frac{s}{s-1}}$ vertices. In particular, when $s=2$ this resolves in a strong sense the conjecture of Mader in 1999 that every $C_4$-free graph has a subdivision of a clique with order linear in the average degree of the original graph. In general, the widely conjectured asymptotic behaviour of the extremal density of $K_{s,t}$-free graphs suggests our result is tight up to the constant $c(s,t)$.Comment: 25 pages, 1 figur