395 research outputs found

    Numerical solutions of three-dimensional non-grey gas radiative transfer using the statistical narrow-band model

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    Peer reviewed: YesNRC publication: Ye

    The Coordination of Centromere Replication, Spindle Formation, and Kinetochore–Microtubule Interaction in Budding Yeast

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    The kinetochore is a protein complex that assembles on centromeric DNA to mediate chromosome–microtubule interaction. Most eukaryotic cells form the spindle and establish kinetochore–microtubule interaction during mitosis, but budding yeast cells finish these processes in S-phase. It has long been noticed that the S-phase spindle in budding yeast is shorter than that in metaphase, but the biological significance of this short S-phase spindle structure remains unclear. We addressed this issue by using ask1-3, a temperature-sensitive kinetochore mutant that exhibits partially elongated spindles at permissive temperature in the presence of hydroxyurea (HU), a DNA synthesis inhibitor. After exposure to and removal of HU, ask1-3 cells show a delayed anaphase entry. This delay depends on the spindle checkpoint, which monitors kinetochore–microtubule interaction defects. Overproduction of microtubule-associated protein Ase1 or Cin8 also induces spindle elongation in HU-arrested cells. The spindle checkpoint-dependent anaphase entry delay is also observed after ASE1 or CIN8 overexpression in HU-arrested cells. Therefore, the shorter spindle in S-phase cells is likely to facilitate proper chromosome–microtubule interaction

    The unsteady-state energy conservation equation for a small spherical particle in LII modeling

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    Peer reviewed: YesNRC publication: Ye

    Numerical study of the effects of gravity on soot formation in laminar coflow methane/air diffusion flames under different air stream velocities

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    Numerical simulations of laminar coflow methane/air diffusion flames at atmospheric pressure and different gravity levels were conducted to gain a better understanding of the effects of gravity on soot formation by using relatively detailed gas-phase chemistry and complex thermal and transport properties coupled with a semi-empirical twoequation soot model. Thermal radiation was calculated using the discrete-ordinates method coupled with a non-grey model for the radiative properties of CO, CO\u2082, H\u2082O, and soot. Calculations were conducted for three coflow air velocities of 77.6, 30, and 5 cm/s to investigate how the coflowing air velocity affects the flame structure and soot formation at different levels of gravity. The coflow air velocity has a rather significant effect on the streamwise velocity and the fluid parcel residence time, especially at reduced gravity levels. The flame height and the visible flame height in general increase with decreasing the gravity level. The peak flame temperature decreases with decreasing either the coflow air stream velocity or the gravity level. The peak soot volume fraction of the flame at microgravity can either be greater or less than that of its normal gravity counterpart, depending on the coflowair velocity.At sufficiently high coflowair velocity, the peak soot volume fraction increases with decreasing the gravity level. When the coflow air velocity is low enough, soot formation is greatly suppressed at microgravity and extinguishment occurs in the upper portion of the flame with soot emission from the tip of the flame owing to incomplete oxidation. The numerical results provide further insights into the intimate coupling between flame size, residence time, thermal radiation, and soot formation at reduced gravity level. The importance of thermal radiation heat transfer and coflow air velocity to the flame structure and soot formation at microgravity is demonstrated for the first time.NRC publication: Ye

    Radiative properties of numerically generated fractal soot aggregates : the importance of configuration averaging

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    The radiative properties of numerically generated fractal soot aggregates were studied using the numerically accurate generalized multisphere Mie-solution method. The fractal aggregates investigated in this study contain 10\u2013600 primary particles of 30 nm in diameter. These fractal aggregates were numerically generated using a combination of the particle-cluster and cluster-cluster aggregation algorithms with fractal parameters representing flame-generated soot. Ten different realizations were obtained for a given aggregate size measured by the number of primary particles. The wavelength considered is 532 nm, and the corresponding size parameter of primary particle is 0.177. Attention is paid to the effect of different realizations of a fractal aggregate with identical fractal dimension, prefactor, primary particle diameter, and the number of primary particles on its orientation-averaged radiative properties. Most properties of practical interest exhibit relatively small variation with aggregate realization. However, other scattering properties, especially the vertical-horizontal differential scattering cross section, are very sensitive to the variation in geometrical configuration of primary particles. Orientationaveraged radiative properties of a single aggregate realization are not always sufficient to represent the properties of random-oriented ensemble of fractal aggregates.Peer reviewed: YesNRC publication: Ye

    Approximating the monomer-dimer constants through matrix permanent

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    The monomer-dimer model is fundamental in statistical mechanics. However, it is #P-complete in computation, even for two dimensional problems. A formulation in matrix permanent for the partition function of the monomer-dimer model is proposed in this paper, by transforming the number of all matchings of a bipartite graph into the number of perfect matchings of an extended bipartite graph, which can be given by a matrix permanent. Sequential importance sampling algorithm is applied to compute the permanents. For two-dimensional lattice with periodic condition, we obtain 0.6627±0.0002 0.6627\pm0.0002, where the exact value is h2=0.662798972834h_2=0.662798972834. For three-dimensional lattice with periodic condition, our numerical result is 0.7847±0.0014 0.7847\pm0.0014, {which agrees with the best known bound 0.7653≤h3≤0.78620.7653 \leq h_3 \leq 0.7862.}Comment: 6 pages, 2 figure
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