Enhanced iron and zinc accumulation in genetically engineered wheat plants using sickle alfalfa (Medicago falcata L.) ferritin gene

Iron deficiency is the most common nutritional disorder, affecting over 30% of the world’s human population. The primary method used to alleviate this problem is nutrient biofortification of crops so as to improve the iron content and its availability in food sources. The over-expression of ferritin is an effective method to increase iron concentration in transgenic crops. For the research reported herein, sickle alfalfa (Medicago falcata L.) ferritin was transformed into wheat driven by the seed-storage protein glutelin GluB-1 gene promoter. The integration of ferritin into the wheat was assessed by PCR, RT-PCR and Western blotting. The concentration of certain minerals in the transgenic wheat grain was determined by inductively coupled plasma-atomic emission spectrometry, the results showed that grain Fe and Zn concentration of transgenic wheat increased by 73% and 44% compared to nontransformed wheat, respectively. However, grain Cu and Cd concentration of transgenic wheat grain decreased significantly in comparison with non-transformed wheat. The results suggest that the over-expression of sickle alfalfa ferritin, controlled by the seed-storage protein glutelin GluB-1 gene promoter, increases the grain Fe and Zn concentration, but also affects the homeostasis of other minerals in transgenic wheat grain

Impact on the Indoor Environment of the Release and Diffusion of TVOC

The release of VOCs by architectural decorative material, furniture and indoor things for use is considered the main reason for indoor environment pollution. The polypropylene Styene-Butadiene Rubber (abbreviation SBR) is regarded as a TVOC release source. The control function of the mechanism mode is put forward according to the process of the TVOC under dry conditions within the room in this study. The function is made up of three parts: the diffusion transport function of VOCS in the material and the release transport function in the interface between air and material and the convention diffusion transport function of the TVOC in the air. The ventilation rate of the local research region was determined after analyzing the VOCS release and diffusion concentrations by using the CFD and VOCS release rates. The average concentration in the different baking temperature was compared. Effective dilution and ventilation methods should be further developed to shorten VOCS release time and reduce its indoor concentration

Simulation Study of Heat Transportation in an Aquifer about Well-water-source Heat Pump

The study of groundwater reinjection, pumping and heat transportation in an aquifer plays an important theoretical role in ensuring the stability of deep-well water reinjection and pumping as well as smooth reinjection. Based on the related conception of underground hydrogeology and the rationale of seepage flow mechanics, a geologic conceptual model of doublet reinjection and a seepage flow model of heat transportation are proposed in this paper. The temperature distribution in the temperature field was obtained by a coupled method of the heat transportation equation and groundwater seepage flow equation fitting for the seepage-affected section. The temperature changes in aquifer and heat storage efficiency are analyzed under different working conditions. All the work referenced above provided references for the popularization and evaluation of well-water source heat pump