De novo assembly and characterization of Camelina sativa transcriptome by paired-end sequencing

published_or_final_versio

The Reliability of Histamine Pharmacodynamic Response Phenotype Classification in Children With Allergic Disease.

We have identified distinct histamine pharmacodynamic response phenotypes in children with allergic disease utilizing histamine iontophoresis with laser Doppler (HILD). These response phenotypes may be relevant in guiding therapeutic decision making for agents targeting the allergic response pathways. However, the reliability of these response phenotypes has not been assessed. Therefore, we performed HILD in children with allergic rhinitis and/or asthma on two to three separate occasions. HILD response-time data were analyzed in NONMEM using a linked effect PKPD model. Examination of observed vs. classified response phenotypes predicted response plots and the sum of residuals. The intraclass correlation coefficient (ICC) was used to determine the reliability of phenotype classification. Eighty-two percent of children exhibited a reliable histamine response phenotype [intraclass correlation coefficient 0.77 (95% CI 0.44–0.93]. These preliminary results suggest moderate reliability of HILD response phenotype in children. Further exploration is needed to determine contributions to phenotype variability

Cellular response of Murine Osteoblasts to Cryopreservation: the influence of attachment to Hydroxyapatite (HA) scaffolds

This paper presents data relevant to the rational design of cryopreservation processes for tissueengineered bone. The effects of cell-scaffold interactions and cell-cell interactions on osteoblastviability and attachment to hydroxyapatite (HA) scaffolds following cryopreservation processing are defined experimentally. It is found that cryopreservation processing detaches osteoblasts from the HAscaffold and that cells suffer significantly more damage than when they are cryopreserved in liquid suspension. Thus, some aspect of cell-surface interaction is detrimental. The detrimental influenceinvolves thermal modulations because when osteoblasts attached to HA are exposed to cryoprotective agents for two-step freezing without temperature change, morphological changes occur but littledetachment or damage is observed. Enhanced post-thaw attachment and viability are realized for higher number densities of osteoblasts on the HA scaffold surface, indicating that some aspect of cellcellinteraction is beneficial. Higher porosity HA scaffolds are better for cell attachment but porosity is not a statistically significant factor for post thaw viability

A potato NOA gene increased salinity tolerance in Arabidopsis thaliana

The purpose of this study is to produce recombinant StNOA1 in transgenic plants and to test its potential role in plant salt stress responses. The newly cloned StNOA1 gene from Solanum tuberosum L. was inserted into AtnOA1 mutant plant genome by Agrobaterium-mediated floral dip method. Transgene integration was verified by polymerase chain reaction (PCR) in 4 different lines of transgenic Atnoa1. Expression of StNOA1 gene was further analyzed by reverse trancription (RT)-PCR. Physiological analyses indicated that the transgenic line TL9 had higher proline, soluble protein and chlorophyll contents as well as lower content of malondialdehyde (MDA) compared to its receptor, Atnoa1 mutant, under salt stress condition. Root elongation and survival rate in TL9 were significantly higher than those in Atnoa1 seedlings under salt stress. Present study proved that StNOA1 participated in Arabidopsis thaliana salt stress responses and increased its salinity tolerance.Keywords: StNOA1 transformation, Solanum tuberosum, Atnoa1 mutant, salt toleranceAfrican Journal of Biotechnology Vol. 9(36), pp. 5869-5878, 6 September, 201

Global small RNA analysis in fast-growing Arabidopsis thaliana with elevated concentrations of ATP and sugars

BACKGROUND: In higher eukaryotes, small RNAs play a role in regulating gene expression. Overexpression (OE) lines of Arabidopsis thaliana purple acid phosphatase 2 (AtPAP2) were shown to grow faster and exhibit higher ATP and sugar contents. Leaf microarray studies showed that many genes involved in microRNAs (miRNAs) and trans-acting siRNAs (tasiRNAs) biogenesis were significantly changed in the fast-growing lines. In this study, the sRNA profiles of the leaf and the root of 20-day-old plants were sequenced and the impacts of high energy status on sRNA expression were analyzed. RESULTS: 9-13 million reads from each library were mapped to genome. miRNAs, tasiRNAs and natural antisense transcripts-generated small interfering RNAs (natsiRNAs) were identified and compared between libraries. In the leaf of OE lines, 15 known miRNAs increased in abundance and 9 miRNAs decreased in abundance, whereas in the root of OE lines, 2 known miRNAs increased in abundance and 9 miRNAs decreased in abundance. miRNAs with increased abundance in the leaf and root samples of both OE lines (miR158b and miR172a/b) were predicted to target mRNAs coding for Dof zinc finger protein and Apetala 2 (AP2) proteins, respectively. Furthermore, a significant change in the miR173-tasiRNAs-PPR/TPR network was observed in the leaves of both OE lines. CONCLUSION: In this study, the impact of high energy content on the sRNA profiles of Arabidopsis is reported. While the abundance of many stress-induced miRNAs is unaltered, the abundance of some miRNAs related to plant growth and development (miR172 and miR319) is elevated in the fast-growing lines. An induction of miR173-tasiRNAs-PPR/TPR network was also observed in the OE lines. In contrast, only few cis- and trans-natsiRNAs are altered in the fast-growing lines.published_or_final_versio

A numerical study of the impact of climate and emission changes on surface ozone over South China in Autumn time in 2000-2050

2012-2013 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishe

Systematic characterization of novel lncRNAs responding to phosphate starvation in Arabidopsis thaliana

published_or_final_versio

Review and comparison of shearography and active thermography for nondestructive evaluation

postprin

Unique walnut-shaped porous MnO<inf>2</inf>/C nanospheres with enhanced reaction kinetics for lithium storage with high capacity and superior rate capability

Unique walnut-shaped porous MnO2/carbon nanospheres via in situ carbonization of amorphous MnO2 nanospheres demonstrate enhanced reaction kinetics for lithium storage.This work is realized in the frame of a program for Changjiang Scholars and Innovative Research Team (IRT_15R52) of Chinese Ministry of Education. B. L. Su acknowledges the Chinese Central Government for an “Expert of the State” position in the Program of the “Thousand Talents” and a Life Membership at the Clare Hall, Cambridge and the financial support of the Department of Chemistry, University of Cambridge. Y. Li acknowledges Hubei Provincial Department of Education for the “Chutian Scholar” program. T. Hasan acknowledges funding from the Royal Academy of Engineering (Graphlex) and an Impact Acceleration Award (GRASS). This work is also financially supported by the National Science Foundation for Young Scholars of China (No. 21301133 and 51302204), International Science & Technology Cooperation Program of China (2015DFE52870) and and Self-determined and Innovative Research Funds of the SKLWUT (2015‐ZD‐7). The authors also would like to thank Dr. Bin-Jie Wang from Shanghai Nanoport (FEI, Shanghai) for TEM analysis, and thank Hang Ping from Wuhan University of Technology for the TGA/DSC tests.This is the author accepted manuscript. The final version is available from the Royal Society of Chemistry via http://dx.doi.org/10.1039/C6TA00594