RiboTagger: Fast and unbiased 16S/18S profiling using whole community shotgun metagenomic or metatranscriptome surveys

10.1186/s12859-016-1378-xBMC Bioinformatics1750

Structure of the human G gamma-A gamma-delta-beta-globin gene locus

We have constructed a physical map of the human G gamma-, A gamma-, delta-, and beta-globin genes. The previously described maps of the fetal and adult beta-like globin genes have been linked to one another by identification of a DNA fragment, generated by BamHI, that contains part of each of the A gamma- and delta-globin genes. The map obtained, which spans more than 40 kilobases, shows the following intergene distances: between G gamma and A gamma, 3500 base pairs; between A gamma and delta, 13,500 base pairs; and between delta and beta, 5500 base pairs. All genes are transcribed from the same DNA strand

The structure of the human β-globin gene in β-thalassaemia

Twenty-one cases of β⁰- and β⁺-thalassaemia have been analysed by restriction endonuclease mapping. In most cases no deletion in the regions surrounding the β- and δ-globin genes could be detected. However, in a single Asian case of β⁰-thalassaemia, homozygous clinically, one of the homologous chromosomes contained a β-globin gene with a deletion of 600 base pairs of DNA and comprising most or all of the 3' end of the structural gene including the EcoRI restriction site within the β-globin coding sequence

The structure of the human β-globin gene in β-thalassaemia

Twenty-one cases of β⁰- and β⁺-thalassaemia have been analysed by restriction endonuclease mapping. In most cases no deletion in the regions surrounding the β- and δ-globin genes could be detected. However, in a single Asian case of β⁰-thalassaemia, homozygous clinically, one of the homologous chromosomes contained a β-globin gene with a deletion of 600 base pairs of DNA and comprising most or all of the 3' end of the structural gene including the EcoRI restriction site within the β-globin coding sequence

Primer-free FISH probes from metagenomics/metatranscriptomics data permit the study of uncharacterised taxa in complex microbial communities

10.1038/s41522-019-0090-9npj Biofilms and Microbiomes511

Physical and genetic analysis of cosmids from the vicinity of the cystic fibrosis locus.

Cosmid libraries have been constructed from DNA of somatic cell hybrid cell lines, each containing a fragment of human chromosome seven and including sequences closely linked to cystic fibrosis (CF). Cosmids containing human DNA as insert were isolated from the library. Three cosmids, when used as probes to total genomic DNA, detected polymorphic loci, each of which was shown to be in strong linkage disequilibrium with CF. Restriction endonuclease digestion of cosmid clones and use of a new, rapid method of chromosome walking based on competitive hybridisation of cosmid inserts has allowed identification of several groups of overlapping cosmids ("contigs") from the vicinity of CF