Investigations of physicochemical properties of bottom-ash materials for use them as secondary raw materials

In this paper chemical content and particle size distribution of bottom-ash material are defined; results of differential thermal and X-ray analyzes are given; processes of phase transformations occurring during heating, are examined for possible use of the waste to produce ceramic products. Studies have shown that effective specific activity of radionuclides in the material under examination would have no effect on radiation safety of the finished product

Identification of new DNA markers of endometrial cancer in patients from the Ukrainian population

Aim: To identify clinically significant molecular markers of endometrial cancer. Materials and Methods: Cancer and normal endometrial tissue samples from 20 patients of the Gynecology Clinic of Odessa State Medical University (Odessa, Ukraine) with confirmed endometrial cancer were compared for SSR and ISSR polymorphisms. Identified polymorphic fragments from anonymous genome regions situated between microsatellite repeats underwent direct DNA sequencing; analysis of their homology to sequences from human genome database has been performed. Results: No significant variability for the microsatellite loci adjacent to the E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 and P53 genes was detected. Search for new molecular markers of endometrial cancer within anonymous DNA sequences located between microsatellite repeats revealed 100 bp and 174 bp polymorphic fragments. These fragments were detected correspondingly in 60% and 35% of patients. 100 bp fragment appeared to be homologous to a region within the NFKB gene, 174 bp fragment – to a sequence within the DDR1 gene. Conclusions: NFKB1 and DDR1 genes may be regarded as potential markers for some types of endometrial cancer. This is a first report about possible association of these genes with endometrial cancer.Цель: идентификация клинически значимых маркеров рака эндометрия. Материалы и Методы: проведен анализ SSR- и ISSR-полиморфизма в образцах опухолей и непораженной ткани эндометрия двадцати пациентов, получавших лечение в клинике гинекологии Одесского государственного медицинского университета (Одесса, Украина). Выполнено секвенирование выявленных полиморфных фрагментов ДНК, локализованных в анонимных участках генома между микросателлитными повторами (ISSR-полиморфизм), осуществлен анализ их гомологии с известными участками ДНК из базы данных генома человека. Результаты: не установлено значительной вариабельности микросателлитных повторов, соседствующих с генами E2F1, BAX, TCF7L2, C-MYC, WNT1, FES, DCC, P27, THRA, APC, CYP19 и P53. В процессе поиска новых маркеров рака эндометрия среди анонимных последовательностей ДНК, локализующихся между микросателлитными повторами, выявлены полиморфные фрагменты длиной 100 и 174 пн. Эти фрагменты присутствовали соответственно у 60 и 35% пациентов . Фрагмент длиной 100 пн оказался гомологичным участку гена NFKB1, а фрагмент длиной 174 пн – участку гена DDR1. Выводы: гены NFKB1 и DDR1 могут рассматриваться в качестве потенциальных маркеров некоторых типов рака эндометрия. Это первое сообщение о возможной ассоциации данных генов с опухолями эндометрия

Synthesis and Physicochemical Properties of SiO[2]-Ta[2]O[5] Thin-Film Systems and Powders

Within the research, SiO[2]-Ta[2]O[5] films from film-forming solutions based on tetraethoxysilane and tantalum chloride (V) are synthesized. Physicochemical patterns and specifics of producing the films are determined by means of IR spectroscopy and thermogravimetric analysis. The processes of SiO[2]-Ta[2]O[5] films formation are studied. Interrelation between SiO[2]-Ta[2]O[5] composition, concentration of incoming components and their physicochemical properties are revealed

Microarray study of gene expression in uterine leiomyoma

Uterine leiomyoma is a most common benign neoplasm in women of reproductive age. It arises from the myometrial compartment of the uterus and may transform in some cases to a malignant phenotype. Aim: To identify the genes involved in pathogenesis of uterine leiomyoma. Methods: We have studied differential gene expression in matched tissue samples of leiomyoma and normal myometrium from the very same people utilizing a cDNA microarray screening method. We also compared our results with previously published microarray data to identify the overlapping gene alterations. Results: Based on this comparison we can divide genes deregulated in our study into two groups. The first group comprises genes that to our knowledge have not been previously reported as deregulated in fibroids: CLDN1, FGF7 (KGF), HNRPM, ISOC1, MAGEC1 (CT7), MAPK12, RFC, TIE1, TNFRSF21 (DR6). The second group consists of genes identified also in previous studies: CCND1 (BCL1), CDKN1A (P21), CRABP2, FN1 and SOX4 (EVI16). In our study FN1 was the most up-regulated gene, occupying the place between the myometrium and fibroids ranging from 2.07 to 3.64, depending of the probe molecule used for detection. Conclusions: Newly identified genes may be regarded as potential diagnostic or prognostic markers of uterine leiomyoma and thus may be very useful as new therapeutic candidates.Лейомиома матки является одним из наиболее распространенных доброкачественных новообразований женской репродуктивной сферы. В некоторых случаях отмечают злокачественную трансформацию данного новообразования. Цель: идентификация генов, вовлеченных в патогенез лейомиомы. Методы: проведен анализ дифференциальной экспрессии генов в образцах лейомиомы и нормального миометрия одних и тех же пациентов методом ДНК-биочип-гибридизации и проведено сравнение полученных результатов с данными, опубликованными ранее. Результаты: выявлены различия в экспрессии ряда генов, которые можно разделить на две группы. Впервые выявлена повышенная экспрессия генов CLDN1, FGF7 (KGF), HNRPM, ISOC1, MAGEC1 (CT7), MAPK12, RFC, TIE1 и TNFRSF21 (DR6) в ткани лейомиомы по сравнению с нормальным миометрием. Ко второй группе можно отнести гены CCND1 (BCL1), CDKN1A (P21), CRABP2, FN1 и SOX4 (EVI16), уже упоминавшиеся в связи с патогенезом лейомиомы в ряде предыдущих исследований. Наибольшим изменением уровня экспрессии (в 2,07–3,64 раз в зависимости от зонда) характеризовался ген фибронектина FN1. Выводы: идентифицированные гены могут рассматриваться в качестве потенциальных диагностических и прогностических маркеров лейомиомы матки

Biotesting as a Method of Evaluating Waste Hazard in Metallic Mineral Mining

As the result of identifying content of each chemical element it has been revealed that metallic mineral wastes have a considerable amount of valuable useful metals. Thus, large-tonnage inorganic wastes are considered to be an additional raw source of metal production. This paper highlights the necessity of supplementary biotesting metallic mineral wastes in order to bring into correlation with corresponding hazard classes and facilitate efficient recycling of these wastes in future. It has been found out that determined in this way waste class can be dumped or used after recycling. It has been also indicated that mill tailings are to be stored according to contained metals without messing up dissimilar metal-containing wastes. After winning metals these wastes are similar to the group of inorganic non-metallic wastes and can be used in building material production, for filling mined-out spaces, in road construction etc

Acid-Base Properties Of Glass Substrate And SiO[2]-Bi[2]O[3]Thin-Film Systems Obtained On It

The article describes an experimental research as a result of which SiO[2]–Bi[2]O[3] films have been synthesized of film-forming solutions based on tetraethoxysilane and bismuth nitrate (III). Acid-base properties of a glass substrate and SiO[2]–Bi[2]O[3] films obtained on it have been studied. The dependency of physical and chemical properties of SiO[2]–Bi[2]O[3] composites on their percentage composition have been revealed

Analyzing the Technology of Using Ash and Slag Waste from Thermal Power Plants in the Production of Building Ceramics

The work describes the problem of impounding and storing ash and slag waste at coal thermal power plants in Russia. Recovery and recycling of ash and slag waste are analyzed. Activity of radionuclides, the chemical composition and particle sizes of ash and slag waste were determined; the acidity index, the basicity and the class of material were defined. The technology for making ceramic products with the addition of ash and slag waste was proposed. The dependencies relative to the percentage of ash and slag waste and the optimal parameters for baking were established. The obtained materials were tested for physical and mechanical properties, namely for water absorption, thermal conductivity and compression strength. Based on the findings, future prospects for use of ash and slag waste were identified

Synthis and Phisical And Chemical; Properties of SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] Thin Film Systems and Powders

The SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] films were synthesized by using film forming solutions having a P[2]O[5] content of up to 30% and B[2]O[3] up to 40%. Properties of the filmforming solutions and binary oxides were examined. The physical and chemical processes occurring in the solution during the heat treatment of films were examined. The conditions for producing films of different thicknesses were determined. The kinetic parameters were calculated

Synthis and Phisical And Chemical; Properties of SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] Thin Film Systems and Powders

The SiO[2]-B[2]O[3] and SiO[2]-P[2]O[5] films were synthesized by using film forming solutions having a P[2]O[5] content of up to 30% and B[2]O[3] up to 40%. Properties of the filmforming solutions and binary oxides were examined. The physical and chemical processes occurring in the solution during the heat treatment of films were examined. The conditions for producing films of different thicknesses were determined. The kinetic parameters were calculated

Acid-Base Properties Of Glass Substrate And SiO[2]-Bi[2]O[3]Thin-Film Systems Obtained On It

The article describes an experimental research as a result of which SiO[2]–Bi[2]O[3] films have been synthesized of film-forming solutions based on tetraethoxysilane and bismuth nitrate (III). Acid-base properties of a glass substrate and SiO[2]–Bi[2]O[3] films obtained on it have been studied. The dependency of physical and chemical properties of SiO[2]–Bi[2]O[3] composites on their percentage composition have been revealed