Production and Characterization of Nickel and Cobalt Binding Proteins

In dieser Arbeit geht es um die Grundlage einer grünen Alternative zur chemischen Aufbereitung gelöster Metallionen. Im Fokus stehen dabei zwei wirtschaftlich wichtige Elemente: Cobalt und Nickel. In einem vorangegangenen Praktikum wurden dafür bereits Peptide mittels Isothermer Titrationskalorimetrie (ITC) untersucht, welche in der Lage waren, diese beiden Metallionen zu binden. Diese Peptide wurden mittels Phagen Display selektiert. Da die zwei Peptide die gewünschten Affinitäten aufwiesen, wurden mit diesen Fusionsproteine konstruiert, wobei die Peptide als Insert, neben weiteren Funktionalitäten, hinzugefügt wurden. Somit entstanden Proteine, welche als Fusionsproteine (FP) bezeichnet werden. Ein Zusätzliches FP mit sieben Histidinen wurde als Positivkontrolle mitgeführt, da Histidin bekanntermaßen beide Elemente binden kan

Characterization of the Binding Behavior of Specific Cobalt and Nickel Ion-Binding Peptides Identified by Phage Surface Display

In recent years, the application focus of phage surface display (PSD) technology has been extended to the identification of metal ion-selective peptides. In previous studies, two phage clones—a nickel-binding one with the peptide motif CNAKHHPRCGGG and a cobalt-binding one with the peptide motif CTQMLGQLCGGG—were isolated, and their binding ability to metal-loaded NTA agarose beads was investigated. Here, the free cyclic peptides are characterized by UV/VIS spectroscopy with respect to their binding capacity for the respective target ion and in crossover experiments for the other ion by isothermal titration calorimetry (ITC) in different buffer systems. This revealed differences in selectivity and affinity. The cobalt-specific peptide is very sensitive to different buffers; it has a 20-fold higher affinity for cobalt and nickel under suitable conditions. The nickel-specific peptide binds more moderately and robustly in different buffers but only selectively to nickel

Characterization of the Binding Behavior of Specific Cobalt and Nickel Ion-Binding Peptides Identified by Phage Surface Display

In recent years, the application focus of phage surface display (PSD) technology has been extended to the identification of metal ion-selective peptides. In previous studies, two phage clones—a nickel-binding one with the peptide motif CNAKHHPRCGGG and a cobalt-binding one with the peptide motif CTQMLGQLCGGG—were isolated, and their binding ability to metal-loaded NTA agarose beads was investigated. Here, the free cyclic peptides are characterized by UV/VIS spectroscopy with respect to their binding capacity for the respective target ion and in crossover experiments for the other ion by isothermal titration calorimetry (ITC) in different buffer systems. This revealed differences in selectivity and affinity. The cobalt-specific peptide is very sensitive to different buffers; it has a 20-fold higher affinity for cobalt and nickel under suitable conditions. The nickel-specific peptide binds more moderately and robustly in different buffers but only selectively to nickel

Potentiation effect of the AMPK activator A-769662 on cardiac myocytes metabolism and survival

Abstract 286 van Poster session 2 Frontiers in CardioVascular Biology, London 30th March – 1st April 2012 Second Congress of the ESC Council on Basic Cardiovascular Science