Weekly changes in body weight and body composition.

<p>(A) Body weight increased by week 2 and remained elevated throughout the six-week hypercaloric high-fat feeding period. (B) Visceral (white bar) and subcutaneous (black bar) adipose tissue calculated as area percent of total tissue. *P<.05 vs. week0; **P<.01 vs. week 0; ***P<.001 vs. week 0.</p

Glucose and FFA plasma levels at three time periods during 24-hr before and after fat feeding.

<p>(A) Plasma glucose and (B) plasma FFAs during 2–4 AM (hash bars), 8–8:30 AM (black bars), and 6–8 PM (white bars). *P<.05 vs. week0; **P<.01 vs. week 0; ***P<.001 vs. week 0.</p

Consistency of the hyperbolic relationship (DI) between insulin sensitivity and insulin secretion.

<p>Solid black hyperbolic curve =  average DI value for week 0; black solid square  =  average DI for week 0; open square  =  average DI for week 2; black solid triangle  =  average DI for week 4; open triangle  =  average DI for week 6. The hyperbola represents the curve on which the animals would have to remain to maintain a constant DI (DI = AIRg.SI = constant) and it is calculated as extrapolation of the average DI for week 0 over a range of insulin sensitivities.</p

Weekly estimates of insulin sensitivity, insulin secretion and whole-body insulin clearance.

<p>(A) SI, (B) AIRg, and (C) MCR of insulin. *P<.05 vs. week 0; **P<.01 vs. week 0; ***P<.001 vs week 0.</p

Spearman’s rank correlation between fasting insulin and fasting FFA.

<p>Spearman’s rank correlation between fasting insulin and fasting FFA.</p

Observed fasting plasma insulin, glucose and FFAs.

<p>Fasting levels of (A) insulin, (B) glucose, and (C) FFA before and after fat feeding. *P<.05 vs. week0; **P<.01 vs. week 0; ***P<.001 vs. week 0.</p