JEB data file G. niger.xlsx

Datasheet1. Sperm velocity (VCL) of sneaker and territorial black goby males in different activating solutions (no seminal fluid, own seminal fluid, the seminal fluid of the opposite tactic)<div>Datasheet2. <i>in vitro</i> fertilization success of sneaker and territorial ejaculates <br><br></div

whole dataset

whole datase

Logistic regression model of fertilization success.

<p>Reported is the logistic regression model of fertilization success of territorial males (proportion of progeny sired by the territorial male) in relation to body weight, number of sneakers and number of females present in the nest at the moment of sampling. SE = standard error.</p

Relationship between body weight and testes weight in sneaker and territorial males of grass goby.

<p>Lines are linear regression lines and dotted lines are their 80% confidence intervals.</p

Sampling details.

<p>The table includes sampling date, number of eggs per nest, total length (TL, cm) and weight (W, g) of territorial male and number of sneakers and females present at the moment of sampling.</p

Relationships between sperm longevity and male chelae asymmetry.

<p>Both variables were √ arcsine-transformed and are thus expressed in degrees (°) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043771#s2" target="_blank">Materials and Methods</a>). The regression line is shown.</p

Relationships between sperm area and relative chelae size.

<p>Relative chelae size is expressed as PC2 scores (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0043771#s2" target="_blank">Methods</a>). The regression line is shown.</p

Relationships between sperm area and date of insemination.

<p>The regression line is shown.</p

Stained freshwater crayfish sperm cells released from spermatophores (40× magnification).

<p>a) Phase contrast microscopy: note the radial nuclear arms or spikes spreading out from the sperm cells at activation; b) Fluorescence microscopy: the same sperm cells under fluorescence light with blue excitation filter (<i>λ</i> = 450–480 nm); living sperm with intact cellular membranes are green, while dead sperm are red.</p

Body traits of male and female crayfish used in mating trials in the two years of study.

<p>Body traits of male and female crayfish used in mating trials in the two years of study.</p