Additional file 3: Table S3. of Comparative transcriptome investigation of global gene expression changes caused by miR156 overexpression in Medicago sativa

Full list of the components included in cellular component, biological process and molecular function. The full list of the GO terms that correspond to the differentially expressed genes in two genotypes of miR156OE alfalfa plants compared to WT. (XLSX 16 kb

Cynanchum sublanceolatum Matsum.

原著和名: コバノカモメヅル科名: ガガイモ科 = Asclepiadaceae採集地: 千葉県 佐倉市 飯重 (下総 佐倉市 飯重)採集日: 1988/10/15採集者: 萩庭丈壽整理番号: JH004782国立科学博物館整理番号: TNS-VS-95478

Additional file 4: Figure S1. of Comparative transcriptome investigation of global gene expression changes caused by miR156 overexpression in Medicago sativa

A representation of the conserved SBP domain (nucleotides) from the genes included in the phylogenetic tree using WebLogo. (TIF 485 kb

Additional file 2: Table S2. of Comparative transcriptome investigation of global gene expression changes caused by miR156 overexpression in Medicago sativa

Differentially expressed genes with significant fold change detected between WT and two miR156OE genotypes (A11and A17). Differentially expressed genes between WT and the two miR156OE genotypes A11a and A17. (XLSX 1067 kb

The Translation Elongation Factor eEF-1Bβ1 Is Involved in Cell Wall Biosynthesis and Plant Development in <em>Arabidopsis thaliana</em>

<div><p>The eukaryotic translation elongation factor eEF-1Bβ1 (EF1Bβ) is a guanine nucleotide exchange factor that plays an important role in translation elongation. In this study, we show that the EF1Bβ protein is localized in the plasma membrane and cytoplasm, and that the transcripts should be expressed in most tissue types in seedlings. Sectioning of the inflorescence stem revealed that EF1Bβ predominantly localizes to the xylem vessels and in the interfascicular cambium. <em>EF1Bβ</em> gene silencing in <em>efβ</em> caused a dwarf phenotype with 38% and 20% reduction in total lignin and crystalline cellulose, respectively. This loss-of-function mutant also had a lower S/G lignin monomer ratio relative to wild type plants, but no changes were detected in a gain-of-function mutant transformed with the <em>EF1Bβ</em> gene. Histochemical analysis showed a reduced vascular apparatus, including smaller xylem vessels in the inflorescence stem of the loss-of-function mutant. Over-expression of <em>EF1Bβ</em> in an <em>eli1</em> mutant background restored a WT phenotype and abolished ectopic lignin deposition as well as cell expansion defects in the mutant. Taken together, these data strongly suggest a role for EF1Bβ in plant development and cell wall formation in Arabidopsis.</p> </div

Additional file 3: of COP9 signalosome subunit 5A affects phenylpropanoid metabolism, trichome formation and transcription of key genes of a regulatory tri-protein complex in Arabidopsis

Table S2. Down-regulated gene expression more than 2-fold in sk372 compared to wild-type control. (XLSX 25 kb

Additional file 16: of Transcriptome profiling of Brassica napus stem sections in relation to differences in lignin content

Table S10. Targeted transcription factors selected for lignin validation using Arabidopsis mutants. (DOCX 25 kb

Total lignin and crystalline cellulose in inflorescence stems of EFβOX and <i>efβ</i> plants.

<p>Lignin is shown relative to that of the WT (A). Cellulose is expressed as µg cellulose mg⁻¹ dry CWM (B). Data presented are means ± SD for three independent experiments, each replicated three times. * and ** indicate significant differences at <i>P</i>≤0.05 and 0.01, respectively.</p

Additional file 3: of Transcriptome profiling of Brassica napus stem sections in relation to differences in lignin content

Table S2A. Differential expression of DH1 vs YN1 less than point 5 (microarray). (XLSX 496 kb

Additional file 17: of Transcriptome profiling of Brassica napus stem sections in relation to differences in lignin content

Table S11. Selected non-TF genes for lignin validation using Arabidopsis mutants. (DOCX 17 kb