The effect of PFC plus carbogen and carbogen alone on survival of mice with intracranial tumours who received a single dose of whole brain radiation.

<p>Kaplan Meier curves were compiled from 3 separate experiments. Mice were randomly assigned to groups of 5 mice. Mice were left untreated (black line), given 4.5Gy of radiation (red line), breathing carbogen (95%O₂/5%CO₂) for 1h immediately prior to irradiation (green line) or injected IV with 1.5cc/kg of a 40% PFC emulsion in addition to breathing carbogen for one hour immediately prior to irradiation (blue line). Only data from animals that died from tumour progression were used. Data from animals that died from other causes were excluded.</p

Different hypoxia levels in intracranial tumours of untreated control mice in the same experiment.

<p><b>(</b>A) and (C): day 14 after implantation; (B) and (D): day 16 after implantation. Photographs are from one experiment with 4 mice (A-D).</p

Irradiation setup for whole brain irradiation of mice.

<p>(A) The mouse is anaesthetized and positioned in a Falcon tube (B) inside a 2 cm thick lead shielding device (C) inside plastic rings (D) inside the aluminium cylinder in the Gammacell 3000 Elan irradiator. (E) Diagrammatic representation of the irradiation setup. This setup allowed us only to do whole brain irradiation; we could not irradiate subcutaneous tumors.</p

Effect of PFC/carbogen and carbogen alone on hypoxia in subcutaneous mouse glioma tumours.

<p>Animals were divided into groups of 5 mice each. (A) Untreated control mice; (B) mice exposed to both PFC emulsions and carbogen and (C) mice exposed to carbogen alone. Tumor-bearing mice were injected IV with 1.5cc/kg of a 40% PFC emulsion and hypoxia was measured using pimonidazole-HCL (PIM). Mice that received carbogen (95%O₂/5%CO₂) for 1h were placed in an air-tight chamber. Photos are representative of 2 separate experiments with 5 mice in each group.</p

Statistical data for Fig 5.

<p>Statistical data for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184250#pone.0184250.g005" target="_blank">Fig 5</a>.</p

Effect of PFC/carbogen and carbogen alone on hypoxia in intracranial mouse glioma tumours.

<p>Animals were divided into groups of 5 mice each. A. Untreated control mice; B. Mice exposed to PFC emulsions; C. Mice exposed to carbogen and D. Mice exposed to both PFC emulsions and carbogen. Tumor-bearing mice were injected IV with 1.5cc/kg of a 40% PFC emulsion and hypoxia was measured using pimonidazole-HCL (PIM). Mice that received carbogen (95%O₂/5%CO₂) for 1h were placed in an air-tight chamber. Photos are representative of 3 separate experiments.</p