Silencing of LIN28B suppressed the migration of SW480 cells.

<p>The cells were transfected with 50 nM NC or si-LIN28B and were allowed to migrate through a Transwell chamber. Representative graphs are presented.</p

LIN28B is significantly overexpressed in colon tumour tissues.

<p>IHC showed that LIN28B was markedly upregulated in tumour tissues compared with the normal mucosa, which demonstrated very little LIN28B expression. Representative graphs are presented.</p

LIN28B overexpression correlated with reduced patient survival and an increased likelihood of tumour recurrence.

<p>(A) Higher LIN28B staining intensity from stage I, II and III colon cancers correlated with reduced patient survival. (B) High LIN28B expression was related to a higher probability of tumour recurrence (p<0.01; log rank test).</p

Repression of LIN28B sensitised SW480 and HCT116 cell to oxaliplatin-induced cytotoxicity.

<p>(A) RT-PCR and Western blot analyses evaluated LIN28B expression levels in Caco2, SW480 and HCT116 cells. (B) SW480 and HCT116 cells were plated in 96-well plates and were incubated with the indicated concentrations of oxaliplatin. Cell viability was assessed using a CCK-8 assay kit after 72 h of exposure to the drug or diluent control. IC₅₀ values were calculated after curve fitting using the XLfit software. (C–D) HCT116 and SW480 cells were transfected with NC or si-LIN28B 24 h prior to oxaliplatin (IC₅₀ value) treatment. The inhibitory rate, normalised to NC, was calculated using CCK-8 absorbance at the indicated time points. The data are represented as the mean fold change ± SE (n = 3; Student's t-test).</p