Rsp5 promotes Gene Activation mediated by 9aaTAD Transcription Factors Oaf1 and Gal4

Nine-amino-acid transactivation domain, 9aaTAD, defines a large superfamily of yeast and mammals transcription factors. The transactivation of the 9aaTAD has been addressed to multiple general co-activators TAF9, MED15, CBP and p300. We demonstrate for the 9aaTAD transcription factors Oaf1 and Gal4 functional and physical interaction with E3-Ubiquitin Ligase Rsp5. The Rsp5-associations with RNA polymerase II and TFIID were reported previously

The organisation and functions of local Ca²⁺ signals

Calcium (Ca2+) is a ubiquitous intracellular messenger, controlling a diverse range of cellular processes, such as gene transcription, muscle contraction and cell proliferation. The ability of a simple ion such as Ca2+ to play a pivotal role in cell biology results from the facility that cells have to shape Ca2+ signals in space, time and amplitude. To generate and interpret the variety of observed Ca2+ signals, different cell types employ components selected from a Ca2+ signalling 'toolkit', which comprises an array of homeostatic and sensory mechanisms. By mixing and matching components from the toolkit, cells can obtain Ca2+ signals that suit their physiology. Recent studies have demonstrated the importance of local Ca2+ signals in defining the specificity of the interaction of Ca2+ with its targets. Furthermore, local Ca2+ signals are the triggers and building blocks for larger global signals that propagate throughout cells

Local and global spontaneous calcium events regulate neurite outgrowth and onset of GABAergic phenotype during neural precursor differentiation

Neural stem cells can generate in vitro progenitors of the three main cell lineages found in the CNS. The signaling pathways underlying the acquisition of differentiated phenotypes in these cells are poorly understood. Here we tested the hypothesis that Ca2+ signaling controls differentiation of neural precursors. We found low-frequency global and local Ca2+ transients occurring predominantly during early stages of differentiation. Spontaneous Ca2+ signals in individual precursors were not synchronized with Ca2+ transients in surrounding cells. Experimentally induced changes in the frequency of local Ca2+signals and global Ca2+ rises correlated positively with neurite outgrowth and the onset of GABAergic neurotransmitter phenotype, respectively. NMDA receptor activity was critical for alterations in neuronal morphology but not for the timing of the acquisition of the neurotransmitter phenotype. Thus, spontaneous Ca2+ signals are an intrinsic property of differentiating neurosphere-derived precursors. Their frequency may specify neuronal morphology and acquisition of neurotransmitter phenotype

Block Crossings in Storyline Visualizations

Storyline visualizations help visualize encounters of the characters in a story over time. Each character is represented by an x-monotone curve that goes from left to right. A meeting is represented by having the characters that participate in the meeting run close together for some time. In order to keep the visual complexity low, rather than just minimizing pairwise crossings of curves, we propose to count block crossings, that is, pairs of intersecting bundles of lines. Our main results are as follows. We show that minimizing the number of block crossings is NP-hard, and we develop, for meetings of bounded size, a constant-factor approximation. We also present two fixed-parameter algorithms and, for meetings of size 2, a greedy heuristic that we evaluate experimentally.Comment: Appears in the Proceedings of the 24th International Symposium on Graph Drawing and Network Visualization (GD 2016

Kodumaa kiriku ja hariduse lugu. Tõine raamat, Uuem aeg : Wene walitsuse algusest meie päiwini. Esimene anne: Esimesed sada aastat Wene kotka warju all

http://tartu.ester.ee/record=b1739079~S1*es

Evolution of the SpaceLiner towards a Reusable TSTO-Launcher

Since a couple of years the DLR launcher systems analysis division is investigating a visionary and extremely fast passenger transportation concept based on rocket propulsion. The fully reusable concept consists of two vertically launched winged stages in parallel arrangement. The space transportation role of the SpaceLiner concept as a TSTO-launcher is now, for the first time, addressed in technical detail. Different mission options to LEO and beyond are traded and necessary modifications of the passenger stage to an unmanned cargo-carrier are investigated and described in this paper. Meanwhile, technical progress of the SpaceLiner ultra-high-speed passenger transport is ongoing at Phase A conceptual design level. Iterative sizings of all major subcomponents in nominal and off-nominal flight conditions have been performed. Potential intercontinental flight routes, taking into account range-safety and sonic boom constraints as well as good reachability from major business centers, are evaluated and flight guidance schemes are established. Alternative passenger cabin and rescue capsule options with innovative morphing shapes were also investigated. The operational and business concept of the SpaceLiner is under definition. The project is on a structured development path and as one key initial step the Mission Requirements Review has been successfully concluded

The subjective experience of habit captured by self-report indexes may lead to inaccuracies in the measurement of habitual action

Commentary on Gardner, B. (2014). A review and analysis of the use of ‘habit’ inunderstanding, predicting and influencing health-related behaviour