4 research outputs found
Direct Assessment of Phytochemicals Inherent in Plant Tissues Using Extractive Electrospray Ionization Mass Spectrometry
An ambient pressure ionization mass
spectrometric strategy called
internal extractive electrospray ionization mass spectrometry (iEESI-MS)
has been developed and applied for direct profiling of labile phytochemicals
inherent in various native plant tissues, including leaves, roots,
and fruits. By passing the electrospray solvent through the plant
tissue, a variety of phytochemicals, such as amino acids, sugars (e.g.,
glucose, sucrose, polysaccharides, etc.), and alkaloids, were continuously
extracted from the sample interior, driven toward the natural/cut
electro-spraying tip, and vaporized into gaseous ions for mass spectrometric
interrogation. Phytochemical patterns obtained by iEESI–MS
permit a rapid differentiation between various species of ginkgo plant
and strawberry maturity stages, as well as characterization of physiological/pathologic
conditions of chlorophytum comosum. Our experimental results further
demonstrate that the established iEESI–MS approach is potentially
useful for direct phytochemomics studies with minimal biodegradation,
allowing elucidation of plant metabolism with high speed, specificity,
and simplicity of analysis
Direct Assessment of Phytochemicals Inherent in Plant Tissues Using Extractive Electrospray Ionization Mass Spectrometry
An ambient pressure ionization mass
spectrometric strategy called
internal extractive electrospray ionization mass spectrometry (iEESI-MS)
has been developed and applied for direct profiling of labile phytochemicals
inherent in various native plant tissues, including leaves, roots,
and fruits. By passing the electrospray solvent through the plant
tissue, a variety of phytochemicals, such as amino acids, sugars (e.g.,
glucose, sucrose, polysaccharides, etc.), and alkaloids, were continuously
extracted from the sample interior, driven toward the natural/cut
electro-spraying tip, and vaporized into gaseous ions for mass spectrometric
interrogation. Phytochemical patterns obtained by iEESI–MS
permit a rapid differentiation between various species of ginkgo plant
and strawberry maturity stages, as well as characterization of physiological/pathologic
conditions of chlorophytum comosum. Our experimental results further
demonstrate that the established iEESI–MS approach is potentially
useful for direct phytochemomics studies with minimal biodegradation,
allowing elucidation of plant metabolism with high speed, specificity,
and simplicity of analysis
Differentiation Using Microwave Plasma Torch Desorption Mass Spectrometry of Navel Oranges Cultivated in Neighboring Habitats
The molecular fingerprinting of intact
fruit samples combined with
statistical data analysis can allow the assessment of fruit quality
and location of origin. Herein, microwave plasma torch desorption
ionization mass spectrometry (MPT-MS) was applied to produce molecular
fingerprints for the juice sac and exocarp of navel oranges cultivated
in three closely located habitats, and the mass spectrometric fingerprints
were differentiated by principal component analysis (PCA). Because
of the relatively high temperature and high ionization efficiency
of MPT, the volatile aroma compounds and semivolatile chemicals in
the navel oranges were sensitively detected and confidently identified
by collision induced dissociation (CID). The limit of detection (LOD)
of MPT-MS for vanillin was 0.119 μg/L, with the relative standard
deviation (RSD, <i>n</i> = 10) of 1.7%. The results showed
that MPT-MS could be a powerful analytical platform for the sensitive
molecular analysis of fruits at molecular level with high chemical
specificity, allowing differentiation between the same sorts grown
in neighboring habitats
Additional file 1: Tables S1-S4. of A network-based predictive gene-expression signature for adjuvant chemotherapy benefit in stage II colorectal cancer
indicate additional results of Cox regression analysis and genes involved in the 11-PPI-mod. Figures S1-S4. show additional information of data processing, feature selection and Kaplan-Meier analysis. (DOC 1262 kb