Cardiopoietic cell therapy for advanced ischemic heart failure: results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial

Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort

The Impact of Rotational Pasture Management for Farm-Bred Fallow Deer (<i>Dama dama</i>) on Fodder Quality in the Context of Animal Welfare

Extensive breeding of farmed cervids, similarly to other livestock, affects the vegetation of grasslands in different seasons. For this reason, the impact of the rotational grazing of fallow deer on the chemical and species composition of the pasture sward was assessed, along with the possibility of using these animals for grasslands conservation. The species composition of the pastures was analysed through the botanical-weight method. A quality index and mineral concentration test by inductively coupled plasma mass spectrometry were used to evaluate the feed. The highest proportion of valuable grasses, such as Dactylis glomerata, Poa pratensis and Lolium perenne, was recorded in the summer pens (65.7–66.1%), while the smallest proportion was recorded in the control area (46.1%). The estimated yield potential was relatively large, from 5.74 to 7.02 t ha−1 dry matter. The lowest total protein content occurred in the control area in the spring and autumn. The summer pens, including the sown one, had a better fodder quality, depending on the species composition. All pens were characterised by a high production potential and similar floristic composition, without the participation of undesirable plant species, which confirms the hypothesis that, under extensive grazing conditions, fallow deer can be used for grassland conservation

Grazing of Native Livestock Breeds as a Method of Grassland Protection in Roztocze National Park, Eastern Poland

The studies were conducted in a forest settlement in Roztocze National Park (eastern Poland). The aim of the study was to evaluate the vegetation of two pastures depending on the type of use in the context of grassland protection, identify the trends of species composition changes, and analyse the yield and nutritional value of the biomass in the context of animal welfare. The studies were conducted on permanent grasslands varying in terms of fertility and location. Both sites were pastures where native breeds of livestock (Polish Lowland sheep of the Uhrusk and Polish Konik) were grazing. The fertile pasture was represented by the developing Lolio-Cynosuretum association, while the poor dry pasture – by a community with Common Bent (Agrostis capillaris L.) and a community with Mouse-Ear Hawkweed (Hieracium pilosella L.). The greatest changes over time were observed in the mowed site in the fertile pasture (increased share of tall grasses) and in the abandoned poor dry pasture (increased share of herbs and weeds). Livestock grazing conducted from 2010 influenced the stabilisation of the species composition. Tree and shrub seedlings were systematically eaten by livestock, which evidences a positive impact of grazing on the preservation of permanent grasslands in Roztocze National Park where forest ecosystems predominate. The assessment of the species composition and yielding indicated that the fertile pasture was characterised by good value while the poor pasture – low or sufficient value. In terms of nutrient yield and content, these pastures were poor or very poor, and their nutritive potential was largely dependent on the meteorological conditions. While the livestock density in the pastures, ranging from 1.1 to 1.5 LSU ha–1 in the years under study, was appropriate, grazing should be limited in the summer months, particularly in periods of drought, by reducing the number of animals or by additional feeding to ensure their welfare

Exome Sequencing Reveals Novel Variants and Expands the Genetic Landscape for Congenital Microcephaly

Congenital microcephaly causes smaller than average head circumference relative to age, sex and ethnicity and is most usually associated with a variety of neurodevelopmental disorders. The underlying etiology is highly heterogeneous and can be either environmental or genetic. Disruption of any one of multiple biological processes, such as those underlying neurogenesis, cell cycle and division, DNA repair or transcription regulation, can result in microcephaly. This etiological heterogeneity manifests in a clinical variability and presents a major diagnostic and therapeutic challenge, leaving an unacceptably large proportion of over half of microcephaly patients without molecular diagnosis. To elucidate the clinical and genetic landscapes of congenital microcephaly, we sequenced the exomes of 191 clinically diagnosed patients with microcephaly as one of the features. We established a molecular basis for microcephaly in 71 patients (37%), and detected novel variants in five high confidence candidate genes previously unassociated with this condition. We report a large number of patients with mutations in tubulin-related genes in our cohort as well as higher incidence of pathogenic mutations in MCPH genes. Our study expands the phenotypic and genetic landscape of microcephaly, facilitating differential clinical diagnoses for disorders associated with most commonly disrupted genes in our cohort