Examples of NIA-Only cDNA Clones and RT–PCR Results

<p>Expression pattern of 19 novel cDNA clones in 16 different cell lines or tissues: unfertilized egg, E3.5 blastocyst, E7.5 whole embryo (embryo plus placenta), E12.5 male mesonephros (gonad plus mesonephros), newborn brain, newborn ovary, newborn kidney, embryonic germ (EG) cell, embryonic stem (ES) cell (maintained as undifferentiated in the presence of LIF), trophoblast stem (TS) cell, mesenchymal stem (MS) cell, osteoblast, neural stem/progenitor (NS) cell, NS differentiated (differentiated neural stem/progenitor cells), and hematopoietic stem/progenitor (HS) cells. Glyceraldegyde-3-phosphate dehydrogenase (GAP-DH) was used as a control. A U number is assigned to each gene in the gene index (see <a href="http://www.plosbiology.org/article/info:doi/10.1371/journal.pbio.0000074#sd002" target="_blank">Dataset S2</a>). The exon number was predicted from alignment with the mouse genome sequence, and the amino acid sequence was predicted with the ORF finder from NCBI.</p

PCA Analysis of EST Frequency

<p>The results were obtained by analyzing 2,812 genes that exceeded 0.1% in at least one library. (A) 3D biplot that shows both cell types (red spheres) and genes (yellow boxes). (B) 2D PCA of cell types. EST frequencies were log-transformed before the analysis. Names of some cells and tissues are abbreviated as follows: 6.5 EP, E6.5 whole embryo (embryo plus placenta); 7.5 EP, E7.5 whole embryo (embryo plus placenta); 8.5 EP, E8.5 whole embryo (embryo plus placenta); 9.5 EP, E9.5 whole embryo (embryo plus placenta); 7.5 E, E7.5 embryonic part only; 7.5 P, E7.5 extraembryonic part only; NbOvary, newborn ovary; NbBrain, newborn brain; NbHeart, newborn heart; NbKidney, newborn kidney; 13.5 VMB, E13.5 ventral midbrain dopamine cells; 12.5 Gonad (F), E12.5 female gonad/mesonephros; 12.5 Gonad (M), E12.5 male gonad/mesonephros; HS (Kit⁻, Sca1⁻), hematopoietic stem/progenitor cells (Lin⁻, Kit⁻, Sca1⁻); HS (Kit⁻, Sca1⁺), hematopoietic stem/progenitor cells (Lin⁻, Kit⁻, Sca1⁺); HS (Kit⁺, Sca1⁻), hematopoietic stem/progenitor cells (Lin⁻, Kit⁺, Sca1⁻); HS (Kit⁺, Sca1⁺), hematopoietic stem/progenitor cells (Lin⁻, Kit⁺, Sca1⁺); and NS-D, differentiated NS cells.</p

Signature Genes for Specific Groups of Early Embryos and Stem Cells

<p>Signature Genes for Specific Groups of Early Embryos and Stem Cells</p