A new optimized method in wellbore to improve gas recovery in shale reservoirs

With the scale development of shale gas, the importance of selecting appropriate deliquification process has become increasingly evident in maintaining well productivity and improving shale gas recovery rate. At present, the preferred deliquification process are macro-control plate method and field experience method. The existing methods can only qualitatively select the deliquification process by considering limited influencing factors, resulting in poor process implementation. Based on the results of error analysis, the Gray model was optimized to calculate the pressure distribution in the shale gas wellbore and determine the applicable pressure limit. The W.Z.B. empirical model, which fully considers the influence of wellbore inclination, is used to calculate the gas-liquid carrying situation and determine the applicable liquid carrying limit. By analyzing the technical limits of five commonly used deliquification processes in the Changning shale gas field, namely, plunger lift, optimizing pipe string, gas lift, foam drainage, and intermittent production, a quantitative optimization method for deliquification processes was established. This method was then used to obtain the optimization chart for deliquification processes in shale gas wells. This method was applied in Well Ning 209-X, where the corresponding optimization chart for deliquification processes was drawn based on the production characteristics of the gas well. By quantitatively optimizing the deliquification processes and adjusting to suitable techniques, it effectively guided the production of the gas well and improved the gas field recovery rate

Association of eNOS Polymorphisms with Anterior Chamber Depth in Han Chinese: Jiangsu Eye Study

Recently, a study reported that single nucleotide polymorphisms (SNP) in endothelial nitric oxide synthase (eNOS) were associated with primary angle closure glaucoma (PACG) in Australian cohort. In this study, we aimed to investigate whether those eNOS SNPs are associated with primary angle closure (PAC) or ocular biometric characteristics such as axial length (AL), anterior chamber depth (ACD), and diopter of spherical power (DS) in Han Chinese. The samples consisted of 232 PAC subjects and 306 controls collected from a population-based prevalence survey conducted in Funing County of Jiangsu, China. The rs3793342 and rs11771443 in eNOS were genotyped by TaqMan-MGB probe using the RT-PCR system. Our data did not identify any association of the eNOS SNPs with PAC. However, the analysis on the quantitative traits of ocular biometrics showed that the ACD of rs11771443 AA and GA carriers is significantly deeper than that of rs11771443 GG carriers (P = 0.0025), even though the AL and DS are not associated with rs11771443 genotypes. Rs3793342 was not associated with any biometric parameters including ACD, AL and DS. In summary, our data indicates that eNOS rs11771443 is associated with ACD and its role in the pathogenesis of PACG warranted further study

Quasi-solid-state electrolyte for rechargeable high-temperature molten salt iron-air battery

Molten salts are a unique type of electrolyte enabling high-temperature electrochemical energy storage (EES) with unmatched reversible electrode kinetics and high ion-conductivities, and hence impressive storage capacity and power capability. However, their high tendency to evaporate and flow at high temperatures challenges the design and fabrication of the respective EES devices in terms of manufacturing cost and cycling durability. On the other hand, most of these EES devices require lithium-containing molten salts as the electrolyte to enhance performances, which not only increases the cost but also demands a share of the already limited lithium resources. Here we report a novel quasi-solid-state (QSS) electrolyte, consisting of the molten eutectic mixture of Na2CO3-K2CO3 and nanoparticles of yttrium stabilized zirconia (YSZ) in a mass ratio of 1:1. The QSS electrolyte has relatively lower volatility in comparison with the pristine molten Na2CO3-K2CO3 eutectic, and therefore significantly suppresses the evaporation of molten salts, thanks to a strong interaction at the interface between molten salt and YSZ nanoparticles at high temperatures. The QSS electrolyte was used to construct an iron-air battery that performed excellently in charge-discharge cycling with high columbic and energy efficiencies. We also propose and confirm a redox mechanism at the three-phase interlines in the negative electrode. These findings can help establish a simpler and more efficient approach to designing low-cost and high-performance molten salt metal-air batteries with high stability and safety

Experimental Study on the Scouring Rate of Cohesive Soil in the Lower Yellow River

The different soil anti scourability in the lower reaches of the Yellow River leads to different scouring and retreating speeds, which has a great influence on river regime evolution. Through the incipient motion scouring test of cemented cohesive soil in the lower reaches of the Yellow River, the physical phenomena of the incipient motion of cohesive soil were expounded, the scouring rate of cohesive soil was calculated, and the relationship between the scouring rate and its influencing factors was established. The results show that when the moisture content of cohesive soil is 43%~61%, the scouring rate is about 0.001~0.03 kg/(m2·s). The scouring rate of cohesive soil with the same particle size varies with the flow shear stress under different deposition duration conditions. Under the same flow rate, the scouring rate of cohesive soil increases with the increase of water content, showing an exponential relationship of increment. Under the same shear stress condition, the scouring rate decreases with the increase of dry density, while the exponential relationship between dry density and scouring rate is not clear when the shear stress is small. With the increase of shear stress, there is an obvious exponential relationship between dry density and scouring rate. Finally, the relationship between the scouring rate and relative residual shear stress was established, and the scouring rate formula suitable for cohesive extremely fine sediment was fitted. The formula can better estimate the scouring rate of the riverbank composed of very fine cohesive sediment and provide support for predicting the scouring and retreating rate of riverbanks in natural rivers

Dynamic Formation Processes and Characteristics of “28 June 2012” Debris Flow in Aizi Valley, Ningnan County, Sichuan Province, China

On 28 June 2012, a large debris flow occurred in Aizi gully, Ningnan County, Sichuan Province, China. It was the most significant debris flow disaster at a construction site in 2012 in China, which left 40 people dead or missing. Field investigations and model calculations were conducted following the event in order to determine dynamic parameters and execute engineering mitigation measures. Analyzing the collected data, it is found that earthquake and drought play a dominant role in debris flow initiation, and from 1 January 1986 to 31 December 2010, 43 earthquakes with a magnitude greater than 3.0 occurred around the study area. Through calculation it was found that the inducive radius of 16 earthquakes was larger than the distance between the epicenter and the study area, and it proves that the study area is frequently affected by seismic activities. Furthermore, on the basis of calculation of rainfall anomaly index H from January 2011 to June 2012, it can be seen that the rainfall of 2011 is extremely low. The percentage of the rainfall anomaly index is almost less than −34%, which indicates that the drought in the basin is serious. Under the influences of repeated seismic activities and continuous droughts, the structure of soil was destroyed and its strength and permeability changed significantly, providing favorable conditions for debris flow initiation. On 28 June 2012, heavy rainfall with the intensity of 23.3 mm/h occurred, and the total amount of precipitation reached 66.1 mm before 6am of 28 June 2012. Debris flow was induced due to the triggering effect of the torrential rain. Studying the causes of the large-scale debris flows in construction sites can significantly facilitate the prevention and mitigation of future debris flow disasters, as well as reduce the potential of hazards caused by debris flows in major engineering areas

Exploring the Attractiveness of Residential Areas for Human Activities Based on Shared E-Bike Trajectory Data

Human activities generate diverse and sophisticated functional areas and may impact the existing planning of functional areas. Understanding the relationship between human activities and functional areas is key to identifying the real-time urban functional areas based on trajectories. Few previous studies have analyzed the interactive information on humans and regions for functional area identification. The relationship between human activities and residential areas is the most representative for urban functional areas because residential areas cover a wide range and are closely connected with human life. The aim of this paper is to propose the CARA (Commuting Activity and Residential Area) model to quantify the correlation between human activities and urban residential areas. In this model, human activities are represented by hot spots extracted by the Gaussian Mixture Model algorithm while residential areas are represented by POI (point of interest) data. The model shows that human activities and residential areas present a logarithmic relationship. The CARA model is further assessed by retrieving urban residential areas in Tengzhou City from shared e-bike trajectories. Compared with the actual map, the accuracy reaches 83.3%, thus demonstrating the model’s reliability and feasibility. This study provides a new method for functional areas identification based on trajectory data, which is helpful for formulating the urban people-oriented policies

Social Group Architecture Based Distributed Ride-Sharing Service in VANET

Lots of traditional distributed ride-sharing services match vehicles with drivers and passengers geographic information only. When urban road congestion situation is particularly serious, such as Beijing's rush hour, the waiting time for the passengers is too long which affects the quality of the service. So we propose a distributed ride-sharing service based on dual Social Group Architecture (SGA). We divide the service into Drivers Social Group Architecture (DSGA) message and Vehicles Social Group Architecture (VSGA) message. Vehicles generate dual SGA messages to complete ride-sharing service. DSGA messages focus on the relation between drivers and passengers. We make a basic geometry matching by generating the DSGA messages. VSGA messages figure out the traffic conditions through a multilevel detection. After generating VSGA messages, the final matching strategy is processed. The low level detection is finished by the limited neighbors to decrease the network consume. Dual SGA messages shorten the waiting time for the passengers and avoid traffic jams. Analysis shows that our scheme enhances the ride-sharing service quality and robust

LRRK2 Deficiency Aggravates Sleep Deprivation-Induced Cognitive Loss by Perturbing Synaptic Pruning in Mice

Mutations of the leucine-rich repeat kinase 2 (LRRK2) gene are associated with pronounced sleep disorders or cognitive dysfunction in neurodegenerative diseases. However, the effects of LRRK2 deficiency on sleep rhythms and sleep deprivation-related cognitive changes, and the relevant underlying mechanism, remain unrevealed. In this study, Lrrk2-/- and Lrrk2+/+ mice were subjected to normal sleep (S) or sleep deprivation (SD). Sleep recording, behavioral testing, Golgi-cox staining, immunofluorescence, and real-time PCR were employed to evaluate the impacts of LRRK2 deficiency on sleep behaviors and to investigate the underlying mechanisms. The results showed that after SD, LRRK2-deficient mice displayed lengthened NREM and shortened REM, and reported decreased dendritic spines, increased microglial activation, and synaptic endocytosis in the prefrontal cortex. Meanwhile, after SD, LRRK2 deficiency aggravated cognitive impairments, especially in the recall memory cued by fear conditioning test. Our findings evidence that LRRK2 modulates REM/NREM sleep and its deficiency may exacerbate sleep deprivation-related cognitive disorders by perturbing synaptic plasticity and microglial synaptic pruning in mice

Hypoxic bone marrow mesenchymal stem cell exosomes promote angiogenesis and enhance endometrial injury repair through the miR-424-5p-mediated DLL4/Notch signaling pathway

Background Currently, bone marrow mesenchymal stem cells (BMSCs) have been reported to promote endometrial regeneration in rat models of mechanically injury-induced uterine adhesions (IUAs), but the therapeutic effects and mechanisms of hypoxic BMSC-derived exosomes on IUAs have not been elucidated. Objective To investigate the potential mechanism by which the BMSCS-derived exosomal miR-424-5p regulates IUA angiogenesis through the DLL4/Notch signaling pathway under hypoxic conditions and promotes endometrial injury repair. Methods The morphology of the exosomes was observed via transmission electron microscopy, and the expression of exosome markers (CD9, CD63, CD81, and HSP70) was detected via flow cytometry and Western blotting. The expression of angiogenesis-related genes (Ang1, Flk1, Vash1, and TSP1) was detected via RT‒qPCR, and the expression of DLL4/Notch signaling pathway-related proteins (DLL4, Notch1, and Notch2) was detected via Western blotting. Cell proliferation was detected by a CCK-8 assay, and angiogenesis was assessed via an angiogenesis assay. The expression of CD3 was detected by immunofluorescence. The endometrial lesions of IUA rats were observed via HE staining, and the expression of CD3 and VEGFA was detected via immunohistochemistry. Results Compared with those in exosomes from normoxic conditions, miR-424-5p was more highly expressed in the exosomes from hypoxic BMSCs. Compared with those in normoxic BMSC-derived exosomes, the proliferation and angiogenesis of HUVECs were significantly enhanced after treatment with hypoxic BMSC-derived exosomes, and these effects were weakened after inhibition of miR-424-5p. miR-424-5p can target and negatively regulate the expression of DLL4, promote the expression of the proangiogenic genes Ang1 and Flk1, and inhibit the expression of the antiangiogenic genes Vash1 and TSP1. The effect of miR-424-5p can be reversed by overexpression of DLL4. In IUA rats, treatment with hypoxic BMSC exosomes and the miR-424-5p mimic promoted angiogenesis and improved endometrial damage. Conclusion The hypoxic BMSC-derived exosomal miR-424-5p promoted angiogenesis and improved endometrial injury repair by regulating the DLL4/Notch signaling pathway, which provides a new idea for the treatment of IUAs