Collagen and bone morphogenetic protein-2 functionalized hydroxyapatite scaffolds induce osteogenic differentiation in human adipose-derived stem cells

Surface modification is one important way to fabricate successful biocompatible materials in bone tissue engineering. Hydroxyapatite (HAp) materials have received considerable attention as suitable bioceramics for manufacturing osseous implants because of their similarity to bone mineral in terms of chemical composition. In this study, the surface of porous HAp scaffold was modified by collagen treatment and bone morphogenetic protein‐2 (BMP‐2) conjugation. The surface modification did not affect the HAp scaffold's bulk properties. No significant difference in compressive strength was found among different scaffolds, with HAp, collagen modified HAp, and collagen–BMP‐2‐functionalized HAp having compressive strengths of 45.8 ± 3.12, 51.2 ± 4.09, and 50.7 ± 3.98 MPa, respectively. In vitro studies were performed to compare adhesion and osteogenic differentiation between human adipose‐derived stem cells (hADSCs) with modified surfaces and those unmodified HAp surfaces. Collagen or BMP‐2 alone was insufficient and that both collagen and BMP‐2 are necessary to get the desired results. The findings suggest the possibility of using three‐dimensional HAp scaffold treated with gold‐standard collagen coating and highly researched BMP‐2 growth factor as a platform to deliver hADSCs. Results of this study could be used to develop treatment strategy for regenerating completely transected models using more synergistic approaches

1960 Chevrolet Brookwood with a dent in the door, photo 5

Electrospun blends of biphasic calcium phosphate (BCP) loaded polyvinyl alcohol (PVA)-gelatin (GE) were created with the aim of fabricating biodegradable scaffolds for bone tissue engineering. The process parameters including the electrical field and tip-to-collector distance (TCD) were investigated. The morphology of these hybrid scaffolds were characterized by scanning electron microscope (SEM). X-ray diffraction (XRD) was used to determine the crystallinity of the membrane. Adhesion of osteoblastic cells (MG-63) onto the BCP loaded PVA/GE composite nano-fiber mat was performed to assess potential of the product as a bone scaffold. This result suggests that the BCP loaded PVA/GE composite nano-fiber mat has a high potential for use in the field of bone regeneration and tissue engineering