Compact Real-time Radiance Fields with Neural Codebook

Reconstructing neural radiance fields with explicit volumetric representations, demonstrated by Plenoxels, has shown remarkable advantages on training and rendering efficiency, while grid-based representations typically induce considerable overhead for storage and transmission. In this work, we present a simple and effective framework for pursuing compact radiance fields from the perspective of compression methodology. By exploiting intrinsic properties exhibiting in grid models, a non-uniform compression stem is developed to significantly reduce model complexity and a novel parameterized module, named Neural Codebook, is introduced for better encoding high-frequency details specific to per-scene models via a fast optimization. Our approach can achieve over 40 $\times$ reduction on grid model storage with competitive rendering quality. In addition, the method can achieve real-time rendering speed with 180 fps, realizing significant advantage on storage cost compared to real-time rendering methods.Comment: Accepted by ICME 202

High-Resolution Volumetric Reconstruction for Clothed Humans

We present a novel method for reconstructing clothed humans from a sparse set of, e.g., 1 to 6 RGB images. Despite impressive results from recent works employing deep implicit representation, we revisit the volumetric approach and demonstrate that better performance can be achieved with proper system design. The volumetric representation offers significant advantages in leveraging 3D spatial context through 3D convolutions, and the notorious quantization error is largely negligible with a reasonably large yet affordable volume resolution, e.g., 512. To handle memory and computation costs, we propose a sophisticated coarse-to-fine strategy with voxel culling and subspace sparse convolution. Our method starts with a discretized visual hull to compute a coarse shape and then focuses on a narrow band nearby the coarse shape for refinement. Once the shape is reconstructed, we adopt an image-based rendering approach, which computes the colors of surface points by blending input images with learned weights. Extensive experimental results show that our method significantly reduces the mean point-to-surface (P2S) precision of state-of-the-art methods by more than 50% to achieve approximately 2mm accuracy with a 512 volume resolution. Additionally, images rendered from our textured model achieve a higher peak signal-to-noise ratio (PSNR) compared to state-of-the-art methods

PRL3-zumab, a first-in-class humanized antibody for cancer therapy

Novel, tumor-specific drugs are urgently needed for a breakthrough in cancer therapy. Herein, we generated a first-in-class humanized antibody (PRL3-zumab) against PRL-3, an intracellular tumor-associated phosphatase upregulated in multiple human cancers, for unconventional cancer immunotherapies. We focused on gastric cancer (GC), wherein elevated PRL-3 mRNA levels significantly correlated with shortened overall survival of GC patients. PRL-3 protein was overexpressed in 85% of fresh-frozen clinical gastric tumor samples examined but not in patient-matched normal gastric tissues. Using human GC cell lines, we demonstrated that PRL3-zumab specifically blocked PRL-3(+), but not PRL-3(–), orthotopic gastric tumors. In this setting, PRL3-zumab had better therapeutic efficacy as a monotherapy, rather than simultaneous combination with 5-fluorouracil or 5-fluorouracil alone. PRL3-zumab could also prevent PRL-3(+) tumor recurrence. Mechanistically, we found that intracellular PRL-3 antigens could be externalized to become “extracellular oncotargets” that serve as bait for PRL3-zumab binding to potentially bridge and recruit immunocytes into tumor microenvironments for killing effects on cancer cells. In summary, our results document a comprehensive cancer therapeutic approach to specific antibody-targeted therapy against the PRL-3 oncotarget as a case study for developing antibodies against other intracellular targets in drug discovery

Targeting codon 158 p53-mutant cancers via the induction of p53 acetylation

Abstract: Gain of function (GOF) DNA binding domain (DBD) mutations of TP53 upregulate chromatin regulatory genes that promote genome-wide histone methylation and acetylation. Here, we therapeutically exploit the oncogenic GOF mechanisms of p53 codon 158 (Arg158) mutation, a DBD mutant found to be prevalent in lung carcinomas. Using high throughput compound screening and combination analyses, we uncover that acetylating mutp53R158G could render cancers susceptible to cisplatin-induced DNA stress. Acetylation of mutp53R158G alters DNA binding motifs and upregulates TRAIP, a RING domain-containing E3 ubiquitin ligase which dephosphorylates IĸB and impedes nuclear translocation of RelA (p65), thus repressing oncogenic nuclear factor kappa-B (NF-ĸB) signaling and inducing apoptosis. Given that this mechanism of cytotoxic vulnerability appears inapt in p53 wild-type (WT) or other hotspot GOF mutp53 cells, our work provides a therapeutic opportunity specific to Arg158-mutp53 tumors utilizing a regimen consisting of DNA-damaging agents and mutp53 acetylators, which is currently being pursued clinically

The Effects of Perceived Cultural and Tourism Public Services on Visitor Satisfaction and Quality of Life: A Multiple Mediation Model

The provision of cultural and tourism public services in tourism destinations exerts a substantial influence on visitor experience and their overall quality of life. The current study applied structural equation modeling to empirically investigate the relationship between perceived cultural and tourism public services of visitors and their quality of life. Additionally, the mediating effects of perceived value, expectancy disconfirmation, and satisfaction in the above-mentioned influence relationship was also explored. In order to collect data, a field survey was conducted in a world heritage site located in Nanjing, Jiangsu Province, China. A total of 550 questionnaires were delivered, out of which 518 valid questionnaires were retained for subsequent analysis. The results demonstrate that perceived cultural and tourism public services of visitors have significant and positive influence on their perceived value, and visitors’ perceived value has a positive effect on their expectancy disconfirmation. Moreover, there is a strong and positive relationship between visitors’ expectancy disconfirmation and their satisfaction and quality of life. Additionally, the mediating effect of perceived value, expectancy disconfirmation, and satisfaction exist, and these three mediating variables serve as full mediators. The present study addresses the theoretical and practical implications, limitations, and potential areas for further research

Multi-Fracture Propagation Considering Perforation Erosion with Respect to Multi-Stage Fracturing in Shale Reservoirs

Shale gas is considered a crucial global energy source. Hydraulic fracturing with multiple fractures in horizontal wells has been a crucial method for stimulating shale gas. During multi-stage fracturing, the fracture propagation is non-uniform, and fractures cannot be induced in some clusters due to the influence of stress shadow. To improve the multi-fracture propagation performance, technologies such as limited-entry fracturing are employed. However, perforation erosion limits the effect of the application of these technologies. In this paper, a two-dimensional numerical model that considers perforation erosion is established based on the finite element method. Then, the multi-fracture propagation, taking into account the impact of perforation erosion, is studied under different parameters. The results suggest that perforation erosion leads to a reduction in the perforation friction and exacerbates the uneven propagation of the fractures. The effects of erosion on multi-fracture propagation are heightened with a small perforation diameter and perforation number. However, reducing the perforation number and perforation diameter remains an effective method for promoting uniform fracture propagation. As the cluster spacing is increased, the effects of erosion on multi-fracture propagation are aggravated because of the weakened stress shadow effect. Furthermore, for a given volume of fracturing fluid, although a higher injection rate is associated with a shorter injection time, the effects of erosion on the multi-fracture propagation are more severe at a high injection rate

Fucose promotes intestinal stem cell-mediated intestinal epithelial development through promoting Akkermansia-related propanoate metabolism

ABSTRACTIntestinal stem cells (ISCs) are critical for the development and rapid turnover of intestinal epithelium. The regulatory effects of gut microbiota and their metabolites on ISCs stemness remain elusive. Fucose has been demonstrated to mediate host–microbe interactions in the intestine. However, the association between fucose, gut bacteria, and ISCs stemness remains unclear. To investigate the effects of fucose on ISCs-mediated intestinal epithelial cells (IECs) development, we administered fucose to 4-week-old mice for 4 weeks. ISCs stemness, IECs proliferation, and differentiation were examined. Variations in gut microbes and metabolism were detected using 16S rDNA sequencing and metabolomic analysis. Fucose was added to the bacterial culture medium to further study its effects on metabolism. Crypts were isolated from the mouse ileum for organoids culture in vitro to evaluate the effects of metabolites and the underlying mechanism. The results showed that fucose accelerated ISCs proliferation and secretory lineage differentiation in mice, whereas antibiotics eliminated these effects. The composition and functions of gut bacteria were altered by fucose treatment, while significant increases in Akkermansia and propanoate metabolism were noted. Propionic acid and propionate have been shown to promote organoid development. Fucose fermentation increases the production of propionic acid in Akkermansia muciniphila and enhances its ability to increase the stemness of ISCs. Moreover, ileal contents from fucose-treated mice promoted organoid development in a Gpr41/Gpr43-dependent manner. Fucose administration activates the Wnt signaling pathway in ISCs, and Wnt inhibitors suppress the effects of fucose. We conclude that fucose accelerates ISC-mediated intestinal epithelial development by promoting Akkermansia-related propanoate metabolism. These findings provide new insights into the promotion of gut homeostasis and the application potential of fucose as a prebiotic

Promoting Effect of L-Fucose on the Regeneration of Intestinal Stem Cells through AHR/IL-22 Pathway of Intestinal Lamina Propria Monocytes

The recovery of the intestinal epithelial barrier is the goal for curing various intestinal injurious diseases, especially IBD. However, there are limited therapeutics for restoring intestinal epithelial barrier function in IBD. The stemness of intestinal stem cells (ISCs) can differentiate into various mature intestinal epithelial cells, thus playing a key role in the rapid regeneration of the intestinal epithelium. IL-22 secreted by CD4+ T cells and ILC3 cells was reported to maintain the stemness of ISCs. Our previous study found that L-fucose significantly ameliorated DSS-induced colonic inflammation and intestinal epithelial injury. In this study, we discovered enhanced ISC regeneration and increased intestinal IL-22 secretion and its related transcription factor AHR in colitis mice after L-fucose treatment. Further studies showed that L-fucose promoted IL-22 release from CD4+ T cells and intestinal lamina propria monocytes (LPMCs) via activation of nuclear AHR. The coculture system of LPMCs and intestinal organoids demonstrated that L-fucose stimulated the proliferation of ISCs through an indirect manner of IL-22 from LPMCs via the IL-22R-p-STAT3 pathway, and restored TNF-α-induced organoid damage via IL-22-IL-22R signaling. These results revealed that L-fucose helped to heal the epithelial barrier by accelerating ISC proliferation, probably through the AHR/IL-22 pathway of LPMCs, which provides a novel therapy for IBD in the clinic