1 research outputs found
Label-Free DNA Sequence Detection through FRET from a Fluorescent Polymer with Pyrene Excimer to SG
A label-free
complex probe composed of a water-soluble fluorescent
pyrene-functionalized polymer, ssDNA, and a nucleic acid stain (SG)
is presented here, which can detect DNA sequence via FRET from pyrene
excimer to SG. Complementary and one-base mismatched strands at nanomolar
concentrations can be distinguished by the examination of the FRET
fluorescence intensity of SG. This novel strategy for detecting DNA
using the fluorescent pyrene-functionalized polymer not only affords
a simple label-free method to detect nucleic acid sequence but also
endows the detection with high sensitivity and selectivity, which
may find wide applications for optical biosensing