A molecular dynamics study on coalbed methane diffusion and water-blocking effects

The extraction efficiency of coalbed methane is affected by many factors. Among them, the fracturing fluid injected during coal seam mining and indigenous liquid water in the coal seam often hinder the diffusion and exploitation of coalbed methane, which leads to the water block effect (WBE). The cause, mechanism, and mitigation methods of the WBE are still not completely understood, and are also the topics of current research. This study was based on a large-scale molecular model which accurately reflects the chemical structure of coal, this study used molecular dynamics (MD) methods to simulate the flow patterns of methane and water in the micro-pores of coal seams, considering the hindering effect of water film on methane. The impact of factors such as water film thickness, pore size, reservoir pressure, and pressure difference on the transport velocities of methane and water film has been quantitatively calculated. The results demonstrate that the existence of water will significantly hinder the diffusion of methane and cause WBE. The pore size and pressure drop will also affect the two-phase flow speed of methane and water. Larger pore size and higher pressure drop favor the flow of methane and water. Through analyzing the structural changes of the water film during its transportation, the patterns of occurrence and disappearance of WBE was investigated. Under the conditions of larger pore sizes and thicker water films, the water phase can maintain a continuous phase during the flow process, which completely seal the pores in the coal seam, and cause the WBE. On the other hand, when the water film is thin or the pore size is small, the attractive interaction between water molecules and coal seam makes it easier for the water phase to break into multiple water molecular clusters and mix with the methane gas phase, thus alleviating the WBE. Based on the nano-scale molecular models, this study quantitatively investigated the effects of pore sizes, pressures differences, and water film on WBE, showing that reducing the interactions between water molecules while enhancing the attraction between water and coal may alleviate WBE. This study can provide some insights on further understanding the mechanisms of WBE and improving the recoverying efficiency of coalbed methane

Association analysis between Acetyl-Coenzyme A Acyltransferase-1 gene polymorphism and growth traits in Xiangsu pigs

IntroductionAcetyl-Coenzyme A Acyltransferase-1 (ACAA1) is a peroxisomal acyltransferase involved in fatty acid metabolism. Current evidence does not precisely reveal the effect of the ACAA1 gene on pig growth performance.MethodsThe present study assessed the mRNA expression levels of the ACAA1 gene in the heart, liver, spleen, lung, kidney of 6-month-old Xiangsu pigs and in the longissimus dorsi muscle at different growth stages (newborn, 6 months and 12 months of age) using RT-qPCR. The relationship between single-nucleotide polymorphisms (SNPs) of ACAA1 gene and growth traits in 6-month-old and 12-month-old Xiangsu pigs was investigated on 184 healthy Xiangsu pigs using Sanger sequencing.ResultsThe ACAA1 gene was expressed in heart, liver, spleen, lung, kidney, and longissimus dorsi muscle of 6-month-old pigs, with the highest level of expression in the liver. ACAA1 gene expression in the longissimus dorsi muscle decreased with age (p < 0.01). In addition, four SNPs were identified in the ACAA1 gene, including exon g.48810 A>G (rs343060194), intron g.51546 T>C (rs319197012), exon g.55035 T>C (rs333279910), and exon g.55088 C>T (rs322138947). Hardy-Weinberg equilibrium (p > 0.05) was found for the four SNPs, and linkage disequilibrium (LD) analysis revealed a strong LD between g.55035 T>C (rs333279910) and g.55088 C>T (rs322138947) (r2 = 1.000). Association analysis showed that g.48810 A>G (rs343060194), g.51546 T>C (rs319197012), g.55035 T>C (rs333279910), and g.55088 C>T (rs322138947) varied in body weight, body length, body height, abdominal circumference, leg and hip circumference and living backfat thickness between 6-month-old and 12-month-old Xiangsu pigs.ConclusionThese findings strongly demonstrate that the ACAA1 gene can be exploited for marker-assisted selection to improve growth-related phenotypes in Xiangsu pigs and present new candidate genes for molecular pig breeding

Validation and Evaluation of a Ship Echo-Based Array Phase Manifold Calibration Method for HF Surface Wave Radar DOA Estimation and Current Measurement

Shore-based phased-array HF radars have been widely used for remotely sensing ocean surface current, wave, and wind around the world. However, phase uncertainties, especially phase distortions, in receiving elements significantly degrade the performance of beam forming and direction-of-arrival (DOA) estimation for phased-array HF radar. To address this problem, the conventional array signal model is modified by adding a direction-based phase error matrix. Subsequently, an array phase manifold calibration method using antenna responses of incoming ship echoes is proposed. Later, an assessment on the proposed array calibration method is made based on the DOA estimations and current measurements that are obtained from the datasets that were collected with a multi-frequency HF (MHF) radar. MHF radar-estimated DOAs using three calibration strategies are compared with the ship directions that are provided by an Automatic Identification System (AIS). Additionally, comparisons between the MHF radar-derived currents while using three calibration strategies and Acoustic Doppler Current Profilers (ADCP)-measured currents are made. The results indicate that the proposed array calibration method is effective in DOA estimation and current measurement for phased-array HF radars, especially in the phase distortion situation

Association Analysis of <i>PRKAA2</i> and <i>MSMB</i> Polymorphisms and Growth Traits of Xiangsu Hybrid Pigs

In this study, Xiangsu hybrid pig growth traits were evaluated via PRKAA2 and MSMB as candidate genes. Sanger sequencing revealed three mutation sites in PRKAA2, namely, g.42101G>T, g.60146A>T, and g.61455G>A, and all these sites were intronic mutations. Moreover, six mutation sites were identified in MSMB: intronic g.4374G>T, exonic g.4564T>C, exonic g.6378G>A, exonic g.6386C>T, intronic g.8643G>A, and intronic g.8857A>G. Association analysis revealed that g.42101G>T, g.60146A>T, g.61455G>A, g.4374G>T, g.4564T>C, g.6378G>A, g.6386C>T, g.8643G>A, and g.8857A>G showed different relationship patterns among body weight, body length, body height, chest circumference, abdominal circumference, tube circumference, and chest depth. Real-time polymerase chain reaction results revealed that the expression of PRKAA2 was highest in the longissimus dorsi muscle, followed by that in the heart, kidney, liver, lung, and spleen. The expression of MSMB was highest in the spleen, followed by that in the liver, kidney, lung, heart, and longissimus dorsi muscle. These results suggest that PRKAA2 and MSMB can be used in marker-assisted selection to improve growth related traits in Xiangsu hybrid pigs, providing new candidate genes for Pig molecular breeding

Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs

The newborn ovary homeobox gene (NOBOX) regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of NOBOX in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that NOBOX is preferentially expressed in the ovary. Six mutations were detected in the NOBOX sequence, including g.1624 T&gt;C, g.1858 G&gt;A, g.2770 G&gt;A, g.2821 A&gt;G, g.5659 A&gt;G, and g.6025 T&gt;A, of which g.1858 G&gt;A was a missense mutation. However, only g.1858 G&gt;A, g.5659 A&gt;G, and g.6025 T&gt;A were significantly associated with litter size traits (p &lt; 0.05). Further prediction of the effect of the missense mutation g.1858 G&gt;A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that NOBOX polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding

Table1_Association analysis between Acetyl-Coenzyme A Acyltransferase-1 gene polymorphism and growth traits in Xiangsu pigs.docx

IntroductionAcetyl-Coenzyme A Acyltransferase-1 (ACAA1) is a peroxisomal acyltransferase involved in fatty acid metabolism. Current evidence does not precisely reveal the effect of the ACAA1 gene on pig growth performance.MethodsThe present study assessed the mRNA expression levels of the ACAA1 gene in the heart, liver, spleen, lung, kidney of 6-month-old Xiangsu pigs and in the longissimus dorsi muscle at different growth stages (newborn, 6 months and 12 months of age) using RT-qPCR. The relationship between single-nucleotide polymorphisms (SNPs) of ACAA1 gene and growth traits in 6-month-old and 12-month-old Xiangsu pigs was investigated on 184 healthy Xiangsu pigs using Sanger sequencing.ResultsThe ACAA1 gene was expressed in heart, liver, spleen, lung, kidney, and longissimus dorsi muscle of 6-month-old pigs, with the highest level of expression in the liver. ACAA1 gene expression in the longissimus dorsi muscle decreased with age (p G (rs343060194), intron g.51546 T>C (rs319197012), exon g.55035 T>C (rs333279910), and exon g.55088 C>T (rs322138947). Hardy-Weinberg equilibrium (p > 0.05) was found for the four SNPs, and linkage disequilibrium (LD) analysis revealed a strong LD between g.55035 T>C (rs333279910) and g.55088 C>T (rs322138947) (r2 = 1.000). Association analysis showed that g.48810 A>G (rs343060194), g.51546 T>C (rs319197012), g.55035 T>C (rs333279910), and g.55088 C>T (rs322138947) varied in body weight, body length, body height, abdominal circumference, leg and hip circumference and living backfat thickness between 6-month-old and 12-month-old Xiangsu pigs.ConclusionThese findings strongly demonstrate that the ACAA1 gene can be exploited for marker-assisted selection to improve growth-related phenotypes in Xiangsu pigs and present new candidate genes for molecular pig breeding.</p

Table_1_Association between polymorphisms in NOBOX and litter size traits in Xiangsu pigs.docx

The newborn ovary homeobox gene (NOBOX) regulates ovarian and early oocyte development, and thus plays an essential role in reproduction. In this study, the mRNA expression level and single nucleotide polymorphism (SNP) of NOBOX in various tissues of Xiangsu pigs were studied to explore the relationship between its polymorphism and litter size traits. Also, bioinformatics was used to evaluate the effects of missense substitutions on protein structure and function. The results revealed that NOBOX is preferentially expressed in the ovary. Six mutations were detected in the NOBOX sequence, including g.1624 T>C, g.1858 G>A, g.2770 G>A, g.2821 A>G, g.5659 A>G, and g.6025 T>A, of which g.1858 G>A was a missense mutation. However, only g.1858 G>A, g.5659 A>G, and g.6025 T>A were significantly associated with litter size traits (p A on protein function revealed that p.V82M is a non-conservative mutation that significantly reduces protein stability and thus alters protein function. Overall, these findings suggest that NOBOX polymorphism is closely related to the litter size of Xiangsu pigs, which may provide new insights into pig breeding.</p