Demographic details for 720 women between the ages of 18 to 40 years, comparing normal subjects with women defined as having polycystic ovary syndrome by NIH criteria (free androgen index greater than 4.5, with irregular menses).

<p>Demographic details for 720 women between the ages of 18 to 40 years, comparing normal subjects with women defined as having polycystic ovary syndrome by NIH criteria (free androgen index greater than 4.5, with irregular menses).</p

Development and characterization of microsatellite primers for Triops granarius (Branchiopoda: Notostraca) using MiSeq technology

Background Next-generation sequencing technology has allowed for the rapid development of microsatellites, neutral polymorphic markers that can be used for the analysis of population structure. Methods and Results In this study, we performed whole-genome sequencing using the Illumina MiSeq system and de novo assembly to design microsatellite primers for Triops granarius populations in Qatar. The developed microsatellites are suitable for future studies of genetic structuring among geographically isolated freshwater pools. A total of 23 different primer pairs produced typical microsatellite results, with each pair successfully amplified in up to 40 individuals. Only five of the loci produced a significant departure from Hardy-Weinberg equilibrium. Conclusions Some of the underlying mechanisms regarding the few loci that deviated from HWE may be further investigated to determine the source of deviation. As T. granarius is the most widely distributed species of the family, the development of these molecular markers would be useful for conducting population genetics and biogeographical studies broadly.Other Information Published in: Molecular Biology Reports License: https://creativecommons.org/licenses/by/4.0See article on publisher's website: http://dx.doi.org/10.1007/s11033-022-07804-4</p

Indicators of online health information usage.

<p>Indicators of online health information usage.</p

Study sample characteristics of 394 Adult Qatari participants, according to gender.

<p>Study sample characteristics of 394 Adult Qatari participants, according to gender.</p

Sources of health information used by Qatari Adults, according to gender.

<p>Sources of health information used by Qatari Adults, according to gender.</p

Corrigendum: Association of complement-related proteins in subjects with and without second trimester gestational diabetes

In the original article, there was an error. One of the funders was missed out in the Acknowledgements. A correction has been made to the Acknowledgements section. “The authors would like to thank Qatar Metabolic Institute, Medical Research Center, Translational Research Institute, Hamad Medical Corporation, Doha, Qatar for supporting the study. And Medical Research Center, Hamad Medical Corporation for the article processing fees support”. The authors apologize for this error and state that this does not change the scientific conclusions of the article in any way. The original article has been updated

Elevated adipsin and reduced C5a levels in the maternal serum and follicular fluid during implantation are associated with successful pregnancy in obese women

Introduction: Complement factors mediate the recruitment and activation of immune cells and are associated with metabolic changes during pregnancy. The aim of this study was to determine whether complement factors in the maternal serum and follicular fluid (FF) are associated with in vitro fertilization (IVF) outcomes in overweight/obese women. Methods: Forty overweight/obese (BMI = 30.8 ± 5.2 kg/m2) female patients, 33.6 ± 6.3 years old, undergoing IVF treatment for unexplained infertility were recruited. Baseline demographic information, including biochemical hormonal, metabolic, and inflammatory markers, and pregnancy outcome, was collected. Levels of 14 complement markers (C2, C4b, C5, C5a, C9, adipsin, mannose-binding lectin, C1q, C3, C3b/iC3b, C4, factor B, factor H, and properdin) were assessed in the serum and FF and compared to IVF outcome, inflammatory, and metabolic markers using multivariate and univariate models. Results: Out of 40 IVF cycles, 14 (35%) resulted in pregnancy. Compared to women with failed pregnancies, women with successful pregnancies had higher levels of adipsin in the serum and FF (p = 0.01) but lower C5a levels (p = 0.05). Serum adipsin levels were positively correlated with circulating levels of vitamin D (R = 0.5, p = 0.02), glucagon (R = 0.4, p = 0.03), leptin (R = 0.4, p = 0.01), resistin (R = 0.4, p = 0.02), and visfatin (R = 0.4, p = 0.02), but negatively correlated with total protein (R = -0.5, p = 0.03). Higher numbers of top-quality embryos were associated with increased levels of C3, properdin, C1q, factors H and B, C4, and adipsin, but with reduced C2 and C5a levels (p ≤ 0.01). Conclusions: Higher adipsin and lower C5a levels in the maternal serum during implantation are potential markers of successful outcome in obese women undergoing IVF-assisted pregnancies.</p