DataSheet1_Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa.PDF

We designed and synthesized recombinant phage nanofibers displaying ToAP2D peptide and investigated their antifungal effect on Sporothrix and the corresponding mechanism. Antimicrobial peptide, ToAP2D, was used as the template. The effect of synthesized recombinant phages on the immune function of CD4+ T lymphocytes in mice was tested using an enzyme-linked immunosorbent assay. The therapeutic effect and safety of recombinant phage administration on Sporothrix-infected BALB/c mice were evaluated based on survival analysis, histopathological changes, and renal and liver functions. The successfully prepared recombinant phage displaying ToAP2D peptides significantly inhibited Sporothrix growth. According to the scanning electron microscopy results, the recombinant phage caused shrinkage and rupture of Sporothrix globosa, leading to leakage of the contents. The Hoechst/propidium iodide double staining test indicated that the recombinant phage could induce cell apoptosis of Sporothrix globosa. The apoptotic pathway might be due to the accumulation of reactive oxygen species in large quantities in cells, activating caspase dependence; this reduced inflammation, prolonged the survival time, and enhanced levels of IFN-γ and IL-17 in mice. We believe that recombinant phage inhibits Sporothrix growth by adjusting the immune response of mice, inducing Sporothrix apoptosis and improving animal survival. This study offers a new approach to preparing antimicrobial peptides.</p

DataSheet_1_A case report of multicentric reticulohistiocytosis with atypical cutaneous presentation.pdf

Multicentric reticulohistiocytosis (MRH) is a rare systemic disorder characterized by histiocytic hyperplasia that mainly involves the skin, mucous membranes, and joints. The typical clinical features include papules, nodules, and arthritis. MRH lesions are relatively extensive but small and scattered. Joint inflammation is characterized by diffuse symmetric polyarthritis as the first symptom, which can be severe and disabling due to destructive joint changes. MRH is easily misdiagnosed in clinical practice. Here, we report the case of an elderly male patient who presented with polyarticular pain in the hip and interphalangeal joints as the first manifestation, followed by the development of large, isolated, bulging skin nodules, which are atypical MRH lesions. This is rare in all MRH case reports, and we made the correct diagnosis by combining skin histopathology, immunohistochemistry, and other clinical examinations. We performed surgical treatment on the local skin lesions of this patient. This case suggests that clinicians should actively correlate the condition and accurately diagnose MRH when encountering atypical skin changes or other diseases as the first symptom and explore the mechanisms of MRH and other clinical manifestations.</p

Estimated gender- and age-specific prevalence of pterygium and number of people living with pterygium in China in 2010.

<p>Estimated gender- and age-specific prevalence of pterygium and number of people living with pterygium in China in 2010.</p

Variations of pterygium prevalence by age, gender and geographic characteristics in China: A systematic review and meta-analysis

<div><p>Background</p><p>Pterygium is a common chronic ophthalmic condition, which may result in significant visual morbidity or lead to blindness in extreme cases. The prevalence of pterygium in China has not been reported at the sub-national level.</p><p>Methods</p><p>In this study, we conducted a systematic review and meta-analysis to estimate the prevalence of pterygium in China. China National Knowledge Infrastructure (CNKI), Wanfang, Chinese Biomedicine Literature Database (CBM-SinoMed), PubMed, Embase and Medline were searched before September 2016. We performed a multilevel mixed-effect meta-regression based on the included studies, our results showed that age, gender and latitude were significantly associated with pterygium prevalence. Based on the final model, the age and gender-specific prevalence of pterygium in 31 Chinese provinces (except Hongkong, Macau and Taiwan) and the whole country was generated.</p><p>Results</p><p>In 2010, the overall prevalence of pterygium in Chinese people aged 15–84 years was 9.84% (95% CI: 6.72–14.14), and the number of pterygium cases in China was 108.65 million (95% CI: 74.23–156.13).</p><p>Conclusions</p><p>In conclusion, the prevalence of pterygium in Chinese population in 2010 was estimated at both the national and provincial levels. The higher burden of pterygium across the country calls for efforts to advocate public health education encouraging people to take appropriate protective measures.</p></div

Gender- and age-specific prevalence of pterygium and numbers of people living with pterygium in 2010.

<p>Gender- and age-specific prevalence of pterygium and numbers of people living with pterygium in 2010.</p

PRISMA flow chart.

<p>PRISMA flow chart.</p

Estimated gender- and age-specific prevalence of pterygium and number of people living with pterygium in China in 2010.

<p>Estimated gender- and age-specific prevalence of pterygium and number of people living with pterygium in China in 2010.</p

Multilevel univariate and multivariate meta-regression models of the various factors related to the prevalence of pterygium.

<p>Multilevel univariate and multivariate meta-regression models of the various factors related to the prevalence of pterygium.</p

The geographical distribution of population, pterygium prevalence and number of people with pterygium in China in 2010.

<p>(a) number of population; (b) overall prevalence of pterygium; (c) number of people with pterygium. The map was created by PGS using ArcMap version 10.1 (Environmental Systems Research Institute, Redlands, CA).</p

Assessing Spatial Accessibility to Maternity Units in Shenzhen, China

<div><p>Background</p><p>With the rapid development of urbanization, pregnant population is growing rapidly in Shenzhen, and it has been a difficulty to serve more and more pregnant women and reduce spatial access disparities to maternity units (MUs). Understanding of the current status of accessibility to MUs is valuable for supporting the rational allocation of MUs in the future.</p><p>Methods</p><p>Based on pregnant population data and MUs data, this study uses a two-step floating catchment area (2SFCA) method based on Geographic Information System (GIS) to analyze the current spatial accessibility to MUs, and then make a comparison between that to public MUs and private MUs.</p><p>Results</p><p>Our analysis of the accessibility to all MUs within a distance of 20 km shows that the accessibilities of the areas alongside the traditional border management line are acceptable, meanwhile highlights some critical areas, such as the west part of Nanshan district and the vast east part of Longgang district. The comparison between spatial accessibility to public MUs and private MUs shows statistically significant difference.</p><p>Discussion</p><p>Results of this study suggest a great effort should be made to improve the equity of spatial accessibility to MUs in Shenzhen. For policy-making, strategy for the siting and allocation of future MUs, no matter public or private, should guarantee the greatest spatial accessibility for every pregnant woman.</p></div