The evolutionarily conserved long non‐coding RNA <i>LINC00261</i> drives neuroendocrine prostate cancer proliferation and metastasis <i>via</i> distinct nuclear and cytoplasmic mechanisms

Metastatic neuroendocrine prostate cancer (NEPC) is a highly aggressive disease, whose incidence is rising. Long noncoding RNAs (lncRNAs) represent a large family of disease- and tissue-specific transcripts, most of which are still functionally uncharacterized. Thus, we set out to identify the highly conserved lncRNAs that play a central role in NEPC pathogenesis. To this end, we performed transcriptomic analyses of donor-matched patient-derived xenograft models (PDXs) with immunohistologic features of prostate adenocarcinoma (AR+/PSA+) or NEPC (AR-/SYN+/CHGA+ ) and through differential expression analyses identified lncRNAs that were upregulated upon neuroendocrine transdifferentiation. These genes were prioritized for functional assessment based on the level of conservation in vertebrates. Here, LINC00261 emerged as the top gene with over 3229-fold upregulation in NEPC. Consistently, LINC00261 expression was significantly upregulated in NEPC specimens in multiple patient cohorts. Knockdown of LINC00261 in PC-3 cells dramatically attenuated its proliferative and metastatic abilities, which are explained by parallel downregulation of CBX2 and FOXA2 through distinct molecular mechanisms. In the cell cytoplasm, LINC00261 binds to and sequesters miR-8485 from targeting the CBX2 mRNA, while inside the nucleus, LINC00261 functions as a transcriptional scaffold to induce SMAD-driven expression of the FOXA2 gene. For the first time, these results demonstrate hyperactivation of the LINC00261-CBX2-FOXA2 axes in NEPC to drive proliferation and metastasis, and that LINC00261 may be utilized as a therapeutic target and a biomarker for this incurable disease

Study on comprehensive index system of production safety accident in chemical enterprise

This paper classified, extracted and characterized the causes of accidents based on accident statistics of chemical enterprises in Jiangsu Province from 2015 to 2019. The internal relationship between the accident result and various causes is analyzed from the aspects of human, machine, material, method, ring and pipe; and using the analytic hierarchy process to establish the comprehensive evaluation index system of the accident, which was applied to predict the accident probability of chemical enterprise. The probability of production safety accident in a chemical enterprise is predicted by using this method, and the probability of the accident is at level of "more likely to happen". The example showed that the analytic hierarchy process has strong operability and good effect, and can be used to predict the accident risk of chemical enterprises. causes and can realize the accident probability prediction of chemical enterprises

Numerical Simulation of Detection Response of Field Effect MOS Transistor to Modulated Terahertz Radiation Signal

The detector response characteristics of the field effect MOS transistor (MOSFET) to modulated terahertz radiation signal are studied in details in this paper by a numerical simulation method developed from the basic hydrodynamic equations which govern the terahertz signal transport in the MOSFET. The numerical method is used with a universal MOSFET carrier density equation coupled to the basic hydrodynamic equations in a much wider range of operation region of MOSFET terahertz detector to develop the numerical simulation program. The simulation results agree well with the existing theory which is only suitable in the nonresonant region. The developed numerical simulation program is also used to analyze the photoresponse characteristics of the MOSFET detector to modulated terahertz radiation in the resonant region, demonstrating potential application in the detector design and optimization from a MOSFET

Finite element modeling of male leg and sportswear: contact pressure and clothing deformation

In clinical practice, fast assessment of contact pressure is usually calculated by Laplace's Law, which neither provides detailed surface geometry for soft materials of the leg, nor offers sufficient predictive power for designing high-performance sportswear. To bridge this gap, this paper describes a finite element (FE) model of sports tights that was developed with a detailed anatomic male leg model to predict the compression effects of high-performance sportswear. Non-linear elastic material was applied on the sportswear material to model the large deformation behavior. Experimental validation on athletes was performed. A reasonable agreement was found in the experimental validation. Suitable profiles were achieved along the height of the leg, in terms of both contact pressure and clothing deformation (true strain or logarithmic strain). The maximum contact pressure (2222 Pa) occurred on the posterior of the ankle, while the maximum principal true strain of the sports tights occurred on the edge of the upper thigh. This study indicates that the proposed FE model is useful for the assessment of contact pressure distribution in sportswear.Institute of Textiles and Clothin

Numerical study on heterodyne terahertz detection in field effect transistor

Numerical method on the heterodyne terahertz detection characteristics of field effect characteristics of field effect transistors is studied in this paper which is based on the hydrodynamic equations which govern the terahertz signal transport in field effect transistors (FETs). A modification is made in an existed numerical tool established by our group by coupling the heterodyne characteristics. This modified numerical tool work well in all operation regions of FETs from sub-threshold to strong inversion and from linear to saturation. And the results are used to demonstrate the potential for using MOS transistors as THz detectors and investigate the optimization of the device structure. (C)2010 Optical Society of Americ

Terahertz Wave Generation and Detection Analysis of Silicon Nanowire MOS Field-Effect Transistor

A complete analysis of Terahertz (THz) wave generation and detection of Silicon Nanowire MOS Field-Effect Transistor (SNFET) is presented in this paper. Based on the developed SNFET-THz device theory, the dependence of THz detection of SNFET on bias and structure parameters are obtained and illustrated. The numerical technique to solve fluid dynamic equation groups, which govern the THz wave transport in SNFET, is also introduced. Based on the developed numerical tool, the THz generation and its instability are demonstrated and analyzed in details. From developed numerical simulation program, the evolution processes of THz plasma wave in generation and detection modes are presented

An optimized design of compression sportswear fabric using numerical simulation and the response surface method

Well-designed compression sportswear can be used for the enhancement of athletic performance and reduction of injury. The material and geometric properties of fabric for compression sportswear are vital in achieving compression effects. This study evaluated and optimized the performance of fabric using the design of experiment (DOE) methods, the response surface method (RSM) and the finite element (FE) model. The evaluation and optimization procedure consisted of three phases. The first phase involved developing the FE model of a fabric tube and cylinder, and validated it by compression experiments involving different fabrics. The second phase evaluated the FE prediction using a five-factor experimental design, namely, hyperelastic properties, thickness, density, friction, and tensile strain. The third and final phase was an optimization process using RSM based on the evaluation results. Findings show that the FE predictions approach closely the results of validation experiments. The nonlinear elastic material properties (hyperelastic properties) and shape dimensions (thickness and tensile strain) of fabric tube were found to be important design factors in influencing contact pressure, while the density of fabric and interface friction coefficient played less important roles. The optimal FE model was determined using RSM analysis. The statistically based FE model was found to be an effective approach for evaluating and optimizing the design parameters of fabric for compression sportswear. The results can be applied to make sportswear that has different compression effects at selected anatomical locations to enhance performance and reduce injuries.Institute of Textiles and ClothingDepartment of Health Technology and Informatic

A generic numerical model for detection of terahertz radiation in MOS field-effect transistors

A generic numerical model which is valid both in the strong inversion regime and sub-threshold regime for the detection of terahertz radiation utilizing Metal-Oxide-Semiconductor (MOS) Field-Effect Transistors (FETs) is developed in this paper. A general carrier density equation and gate leakage current are coupled to the basic hydrodynamic equations which govern the electron transport in the 2D channel of the MOS field-effect transistor to obtain the numerical solution; a progress-based photo-response signal of the terahertz radiation of MOSFET is calculated. The simulation results are compared with existing analytical results, proving the validity of the proposed numerical model and overcoming limitations of the analytical theories. (C) 2010 Elsevier Ltd. All rights reserved