8,168 research outputs found
Probing Transverse Momentum Broadening via Dihadron and Hadron-jet Angular Correlations in Relativistic Heavy-ion Collisions
Dijet, dihadron, hadron-jet angular correlations have been reckoned as
important probes of the transverse momentum broadening effects in relativistic
nuclear collisions. When a pair of high-energy jets created in hard collisions
traverse the quark-gluon plasma produced in heavy-ion collisions, they become
de-correlated due to the vacuum soft gluon radiation associated with the
Sudakov logarithms and the medium-induced transverse momentum broadening. For
the first time, we employ the systematical resummation formalism and establish
a baseline calculation to describe the dihadron and hadron-jet angular
correlation data in and peripheral collisions where the medium effect
is negligible. We demonstrate that the medium-induced broadening and the so-called jet quenching parameter can be
extracted from the angular de-correlations observed in collisions. A
global analysis of dihadron and hadron-jet angular correlation data
renders the best fit for a
quark jet at RHIC top energy. Further experimental and theoretical efforts
along the direction of this work shall significantly advance the quantitative
understanding of transverse momentum broadening and help us acquire
unprecedented knowledge of jet quenching parameter in relativistic heavy-ion
collisions.Comment: 6 pages, 3 figure
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system.
Precise genetic modifications in model animals are essential for biomedical research. Here, we report a programmable "base editing" system to induce precise base conversion with high efficiency in zebrafish. Using cytidine deaminase fused to Cas9 nickase, up to 28% of site-specific single-base mutations are achieved in multiple gene loci. In addition, an engineered Cas9-VQR variant with 5'-NGA PAM specificities is used to induce base conversion in zebrafish. This shows that Cas9 variants can be used to expand the utility of this technology. Collectively, the targeted base editing system represents a strategy for precise and effective genome editing in zebrafish.The use of base editing enables precise genetic modifications in model animals. Here the authors show high efficient single-base editing in zebrafish using modified Cas9 and its VQR variant with an altered PAM specificity
Adaptive computation of multiscale entropy and its application in EEG signals for monitoring depth of anesthesia during surgery
Entropy as an estimate of complexity of the electroencephalogram is an effective parameter for monitoring the depth of anesthesia (DOA) during surgery. Multiscale entropy (MSE) is useful to evaluate the complexity of signals over different time scales. However, the limitation of the length of processed signal is a problem due to observing the variation of sample entropy (SE) on different scales. In this study, the adaptive resampling procedure is employed to replace the process of coarse-graining in MSE. According to the analysis of various signals and practical EEG signals, it is feasible to calculate the SE from the adaptive resampled signals, and it has the highly similar results with the original MSE at small scales. The distribution of the MSE of EEG during the whole surgery based on adaptive resampling process is able to show the detailed variation of SE in small scales and complexity of EEG, which could help anesthesiologists evaluate the status of patients.The Center for Dynamical Biomarkers and Translational Medicine, National Central University, Taiwan
which is sponsored by National Science Council (Grant Number: NSC 100-2911-I-008-001). Also, it was supported by Chung-Shan Institute of Science & Technology in Taiwan (Grant Numbers: CSIST-095-V101 and CSIST-095-V102). Furthermore, it was supported by the National Science Foundation of China (No.50935005)
Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9.
The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5'-NGG-3' protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences. Here, we report that a Cas9 ortholog from Staphylococcus aureus (SaCas9), and its KKH variant, successfully induced targeted mutagenesis with high frequency in zebrafish. Confirming previous findings, the SpCas9 variant, VQR, can also induce targeted mutations in zebrafish. Bioinformatics analysis of these new Cas targets suggests that the number of available target sites in the zebrafish genome can be greatly expanded. Collectively, the expanded target repertoire of Cas9 in zebrafish should further facilitate the utility of this organism for genetic studies of vertebrate biology
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