Arousal regulates frequency tuning in primary auditory cortex.

Changes in arousal influence cortical sensory representations, but the synaptic mechanisms underlying arousal-dependent modulation of cortical processing are unclear. Here, we use 2-photon Ca2+ imaging in the auditory cortex of awake mice to show that heightened arousal, as indexed by pupil diameter, broadens frequency-tuned activity of layer 2/3 (L2/3) pyramidal cells. Sensory representations are less sparse, and the tuning of nearby cells more similar when arousal increases. Despite the reduction in selectivity, frequency discrimination by cell ensembles improves due to a decrease in shared trial-to-trial variability. In vivo whole-cell recordings reveal that mechanisms contributing to the effects of arousal on sensory representations include state-dependent modulation of membrane potential dynamics, spontaneous firing, and tone-evoked synaptic potentials. Surprisingly, changes in short-latency tone-evoked excitatory input cannot explain the effects of arousal on the broadness of frequency-tuned output. However, we show that arousal strongly modulates a slow tone-evoked suppression of recurrent excitation underlying lateral inhibition [H. K. Kato, S. K. Asinof, J. S. Isaacson, Neuron, 95, 412-423, (2017)]. This arousal-dependent "network suppression" gates the duration of tone-evoked responses and regulates the broadness of frequency tuning. Thus, arousal can shape tuning via modulation of indirect changes in recurrent network activity

Trellis decoding complexity of linear block codes

In this partially tutorial paper, we examine minimal trellis representations of linear block codes and analyze several measures of trellis complexity: maximum state and edge dimensions, total span length, and total vertices, edges and mergers. We obtain bounds on these complexities as extensions of well-known dimension/length profile (DLP) bounds. Codes meeting these bounds minimize all the complexity measures simultaneously; conversely, a code attaining the bound for total span length, vertices, or edges, must likewise attain it for all the others. We define a notion of “uniform” optimality that embraces different domains of optimization, such as different permutations of a code or different codes with the same parameters, and we give examples of uniformly optimal codes and permutations. We also give some conditions that identify certain cases when no code or permutation can meet the bounds. In addition to DLP-based bounds, we derive new inequalities relating one complexity measure to another, which can be used in conjunction with known bounds on one measure to imply bounds on the others. As an application, we infer new bounds on maximum state and edge complexity and on total vertices and edges from bounds on span lengths

Bombardier Enables Delivery of Short-Form Bomanins in the Drosophila Toll Response.

Toll mediates a robust and effective innate immune response across vertebrates and invertebrates. In Drosophila melanogaster, activation of Toll by systemic infection drives the accumulation of a rich repertoire of immune effectors in hemolymph, including the recently characterized Bomanins, as well as the classical antimicrobial peptides (AMPs). Here we report the functional characterization of a Toll-induced hemolymph protein encoded by the bombardier (CG18067) gene. Using the CRISPR/Cas9 system to generate a precise deletion of the bombardier transcriptional unit, we found that Bombardier is required for Toll-mediated defense against fungi and Gram-positive bacteria. Assaying cell-free hemolymph, we found that the Bomanin-dependent candidacidal activity is also dependent on Bombardier, but is independent of the antifungal AMPs Drosomycin and Metchnikowin. Using mass spectrometry, we demonstrated that deletion of bombardier results in the specific absence of short-form Bomanins from hemolymph. In addition, flies lacking Bombardier exhibited a defect in pathogen tolerance that we trace to an aberrant condition triggered by Toll activation. These results lead us to a model in which the presence of Bombardier in wild-type flies enables the proper folding, secretion, or intermolecular associations of short-form Bomanins, and the absence of Bombardier disrupts one or more of these steps, resulting in defects in both immune resistance and tolerance

Time evolution, cyclic solutions and geometric phases for general spin in an arbitrarily varying magnetic field

A neutral particle with general spin and magnetic moment moving in an arbitrarily varying magnetic field is studied. The time evolution operator for the Schr\"odinger equation can be obtained if one can find a unit vector that satisfies the equation obeyed by the mean of the spin operator. There exist at least $2s+1$ cyclic solutions in any time interval. Some particular time interval may exist in which all solutions are cyclic. The nonadiabatic geometric phase for cyclic solutions generally contains extra terms in addition to the familiar one that is proportional to the solid angle subtended by the closed trace of the spin vector.Comment: revtex4, 8 pages, no figur

The land plant-specific MIXTA-MYB lineage is implicated in the early evolution of the plant cuticle and the colonization of land.

The evolution of a lipid-based cuticle on aerial plant surfaces that protects against dehydration is considered a fundamental innovation in the colonization of the land by the green plants. However, key evolutionary steps in the early regulation of cuticle synthesis are still poorly understood, owing to limited studies in early-diverging land plant lineages. Here, we characterize a land plant specific subgroup 9 R2R3 MYB transcription factor MpSBG9, in the early-diverging land plant model Marchantia polymorpha, that is homologous to MIXTA proteins in vascular plants. The MpSBG9 functions as a key regulator of cuticle biosynthesis by preferentially regulating expression of orthologous genes for cutin formation, but not wax biosynthesis genes. The MpSBG9 also promotes the formation of papillate cells on the adaxial surface of M. polymorpha, which is consisitent with its canonical role in vascular plants. Our observations imply conserved MYB transcriptional regulation in the control of the cutin biosynthesis pathway as a core genetic network in the common ancestor of all land plants, implicating the land plant-specific MIXTA MYB lineage in the early origin and evolution of the cuticle

Readability of Dutch online patient-directed health information on breast reconstruction

Background The Netherlands and Belgium are among the top five worldwide of highest incidence in breast cancer, leading to an increase in post-mastectomy reconstruction interest. This study aims to analyze readability of Dutch-written online patient-directed information on breast reconstruction. Methods An online patient query for the term borstreconstructie was simulated using the largest search engine, Google. Content from the 10 most popular web sites from the Netherlands and Belgium was collected and formatted into plain text. Readability level assessment was performed using four available tools for the Dutch language: Accesibility.nl, Klinkende Taal, Texamen, and WizeNote, which measure readability according to the Common European Framework of Reference for Languages (CEFR). Results Of the 20 websites (10 Dutch and Belgian), breast reconstruction content was mainly written in B2/C1 and exceeded the recommended B1 level. The readability tool "Klinkende Taal" was found to have the lowest average CEFR level at B2 (average 4.01, 2.40-5.00), WizeNote (average 4.57, 3.00-5.00), and Accesibility.nl (average 4.58, 3.40-5.50). Both had a CEFR level B2/C1, while Texamen scored the highest average at C1 (average 4.77, 3.60-5.25). Kiesbeter.nl, a Dutch government web site, was found to comply with the recommended B1 level on their breast reconstruction information. Conclusions Readability of online health information on breast reconstruction was generally found to be too advanced for the lay population. Therefore, it may hold benefit for patient-directed health information to be analyzed and revised in order to tailor the information to the targeted population

The Utility of Outcome Studies in Plastic Surgery

Summary: Outcome studies help provide the evidence-based science rationalizing treatment end results that factor the experience of patients and the impact on society. They improve the recognition of the shortcoming in clinical practice and provide the foundation for the development of gold standard care. With such evidence, health care practitioners can develop evidence-based justification for treatments and offer patients with superior informed consent for their treatment options. Furthermore, health care and insurance agencies can recognize improved cost-benefit options in the purpose of disease prevention and alleviation of its impact on the patient and society. Health care outcomes are ultimately measured by the treatment of disease, the reduction of symptoms, the normalization of laboratory results and physical measures, saving a life, and patient satisfaction. In this review, we outline the tools available to measure outcomes in plastic surgery and subsequently allow the objective measurements of plastic surgical conditions. Six major outcome categories are discussed: (1) functional measures; (2) preference-based measures and utility outcome scores; (3) patient satisfaction; (4) health outcomes and time; (5) other tools: patient-reported outcome measurement information system, BREAST-Q, and Tracking Operations and Outcomes for Plastic Surgeons; and (6) cost-effectiveness analysis. We use breast hypertrophy requiring breast reduction as an example throughout this review as a representative plastic surgical condition with multiple treatments available

Fast solar electrons, interplanetary plasma and km-wave type-III radio bursts observed from the IMP-6 spacecraft

IMP-6 spacecraft observations of low frequency radio emission, fast electrons, and solar wind plasma are used to examine the dynamics of the fast electron streams which generate solar type-III radio bursts. Of twenty solar electron events observed between April, 1971 and August, 1972, four were found to be amenable to detailed analysis. Observations of the direction of arrival of the radio emission at different frequencies were combined with the solar wind density and velocity measurements at 1 AU to define an Archimedean spiral trajectory for the radio burst exciter. The propagation characteristics of the exciter and of the fast electrons observed at 1 AU were then conpared. We find that: (1) the fast electrons excite the radio emission at the second harmonic; (2) the total distance travelled by the electrons was between 30 and 70% longer than the length of the smooth spiral defined by the radio observations; (3) this additional distance travelled is the result of scattering of the electrons in the interplanetary medium; (4) the observations are consistent with negligible true energy loss by the fast electrons.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43740/1/11207_2004_Article_BF00153227.pd