3 research outputs found

    Environmental Risk Assessment of Water Resources in Arid and Semi-arid Lands Using Bioassays Systems

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    Abstract: Control of effluents has traditionally been regulated using methods with unspecified responses and measuring global parameters, such as the chemical oxygen demand (COD) or Biological Oxygen Demand (BOD). Moreover, information about biological effects is unknown. Thus, the introduction of in vitro assays using mammalian cells to investigate the presence of environmental contaminants is more than needed. At first, physicochemical parameters of municipal wastewater samples from Tunisia were estimated. On a further step, in vitro bioassays systems were applied intending to find out the biological effects of these samples on mammalian cells. Results using the E-screen assay reveal that the wastewater samples induced estrogenic activity at different concentrations in 6 days incubation period. Besides, the same samples were able to generate stress response on animal cells. These findings correlate with the physicochemical parameters data and thus evaluate the efficiency of treatment system to remove such toxic compounds
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