Development of Animal Model for Studying Deep Second-Degree Thermal Burns

Thermal lesions were produced in 12 male Wistar rats, positioning a massive aluminum bar 10 mm in diameter (51 g), preheated to 99°C ± 2°C/10 min. on the back of each animal for 15 sec. After 7, 14, 21, and 28 days, animals were euthanized. The edema intensity was mild, with no bubble and formation of a thick and dry crust from the 3rd day. The percentage of tissue shrinkage at 28 days was 66.67 ± 1.66%. There was no sign of infection, bleeding, or secretion. Within 28 days reepithelialization was incomplete, with fibroblastic proliferation and moderate fibrosis and presence of modeled dense collagen fibers. It is concluded that the model established is applicable in obtaining deep second-degree thermal burns in order to evaluate the healing action of therapeutic agents of topical use

Topical Application Effect of the Isolectin Hydrogel (Cramoll 1,4) on Second-Degree Burns: Experimental Model

This study aimed at evaluating the use of hydrogel isolectin in the treatment of second-degree burns. Twenty male rats were randomly divided into two groups (G1 = treatment with hydrogel containing 100 μg/mL Cramoll 1,4 and G2 = Control, hydrogel). After 7, 14, 21, 28, and 35 days, animals were euthanized. On the 7th day, G1 showed intense exudates, necrosis and edema. On the 14th day, G1 showed tissue reepithelialization and moderate autolysis. On the 21st day, G1 showed intense fibroblastic proliferation, presence of dense collagen, and moderate fibrosis. On the 28th day, G1 showed complete tissue epithelialization. On the 35th day, G1 showed modeled dense collagen. The significant wound contraction was initiated from day, 14 in the G1. There were no significant differences in biochemical and hematological parameters analyzed. These results extend the potential of therapeutic applications for Cramoll 1,4 in the treatment of thermal burns

Radiolabeling of Cramoll 1,4: Evaluation of the Biodistribution

The cramoll 1,4 is a well-studied lectin. However, few studies about its biodistribution have been done before. In this study, we radiolabeled the cramol 1,4 with Tc-99m and analyzed the biodistribution. The results showed that the cramol has an abnormal uptake by the bowel with reflections on its clearance mechanism

Lectinas de Cratylia mollis (Cramoll-1,4) : estudo de uma formulação em gel irradiado e seu efeito no processo de reparação tecidual in vivo

Camaratu ou feijão Camaratuba é uma leguminosa da região semi-árida do Nordeste do Brasil. Lectinas de Cratylia mollis (Cramoll-1,4), foram estruturalmente caracterizadas e seus efeitos biológicos e farmacológicos continuam sob investigação, pois seu uso como um produto biofarmacêutico requer maior aprofundamento. O objetivo deste estudo foi propor e caracterizar uma formulação em gel irradiada de Cramoll-1,4, quanto à preservação das propriedades in vitro das lectinas, sob o efeito de radiação gama e sua atividade em processos de reparo tecidual in vivo. Inicialmente foi feita a extração e purificação da Cramoll-1,4, foi desenvolvida uma formulação em gel utilizando-se Carbopol como veículo, que foi irradiada com raios gama de Co60 em uma dose de 7,549 kGy h-1. Para avaliação da biodistribuição, a Cramoll-1,4 foi incubada com Tc-99 e para caracterizar a marcação foi feita cromatografia em camada fina. Em seguida o gel foi empregado em protocolos de reparação tecidual in vivo, mais especificamente tecido ósseo e epitelial. A formulação proposta na concentração de 300 μg manteve a atividade hemaglutinante in vitro, sugerindo um papel de ―proteção‖, dificultando a inativação da Cramoll-1,4 pela radiação. A biodistribuição do referido gel foi avaliada a partir da marcação com tecnécio, mostrou que esta lectina foi captada pelo intestino, metabolizada pelo fígado e excretada pelos rins e não ultrapassa a barreira hematoencefálica. Quanto à aplicação tópica do gel irradiado da Cramoll-1,4 na concentração de 300 μg em defeitos ósseos foi observado que a formulação, promoveu um estímulo imediato no tecido conjuntivo mole, dificultando, entretanto, o contato direto entre a formulação e o tecido ósseo. Assim, observou-se a formação de tecido fibroso e não a esperada neoformação óssea. Em paralelo, a aplicação tópica regular do gel irradiado da Cramoll-1,4 na concentração de 100 μg para o tratamento de queimaduras cutâneas de segundo grau experimentalmente induzidas, acelerou os processos de granulação, reepitelização e retração da ferida.Lectins Cratylia mollis (Cramoll-1,4) have been structurally characterized and their biological and pharmacological effects remain under investigation, since its use as a biopharmaceutical requires further elaboration. The objective of this study was to propose and characterize a gel formulation radiated Cramoll-1,4, and the preservation of properties of lectins in vitro, under the influence of gamma radiation and its activity in tissue repair processes in vivo (skin and bone). Initially, we made the extraction and purification of Cramoll-1,4, was developed a gel formulation using Carbopol as a vehicle, which was irradiated with 60Co gamma rays at a dose of 7,549 kGy h-1. To evaluate the biodistribution, Cramoll-1,4 was incubated with Tc-99 and to characterize the marking was made thin-layer chromatography. Then the gel was used in protocols for tissue repair in vivo, specifically bone tissue and epithelial. The proposed formulation at a concentration of 300 μg retained the hemagglutinating activity in vitro, suggesting a role of "protection", making the inactivation of Cramoll-1,4 radiation. The biodistribution of this gel was evaluated from the labeling with technetium showed that this lectin was captured by the intestine, metabolized by the liver and excreted by the kidneys and does not cross the blood brain barrier. The application of the topical gel irradiated Cramoll-1,4 at a concentration of 300 μg in bone defects was observed that the formulation, promoted an immediate boost in soft tissue, making it difficult, however, direct contact between the formulation and bone tissue. Thus, we observed the formation of fibrous tissue and not the expected new bone formation. In parallel, regular topical application of gel irradiated Cramoll-1,4 at a concentration of 100 μg for the treatment of second-degree skin burns, experimentally induced, sped up the process of granulation, reepithelialization and wound contraction