10 research outputs found
High concentrations of glucose reduce the oxidative metabolism of dog neutrophils in vitro
Background: Dogs are commonly affected by hyperglycemic conditions. Hyperglycemia compromises the immune response and favors bacterial infections; however, reports on the effects of glucose on neutrophil oxidative metabolism and apoptosis are conflicting in humans and rare in dogs. Considering the many complex factors that affect neutrophil oxidative metabolism in vivo, we investigated in vitro the specific effect of high concentrations of glucose on superoxide production and apoptosis rate in neutrophils from healthy dogs.Results: The capacity of the neutrophils to reduce tetrazolium nitroblue decreased significantly in the higher concentration of glucose (15.13 ± 9.73% (8 mmol/L) versus 8.93 ± 5.71% (16 mmol/L)). However, there were no changes in tetrazolium nitroblue reduction at different glucose concentrations when the neutrophils were first activated with phorbol myristate acetate. High concentrations of glucose did not affect the viability and apoptosis rate of canine neutrophils either with or without prior camptothecin stimulation. This study provides the first evidence that high concentrations of glucose inhibit the oxidative metabolism of canine neutrophils in vitro in a manner similar to that which occurs in humans, and that the decrease in superoxide production did not increase the apoptosis rate.Conclusions: A high concentration of glucose reduces the oxidative metabolism of canine neutrophils in vitro. It is likely that glucose at high concentrations rapidly affects membrane receptors responsible for the activation of NADPH oxidase in neutrophils; therefore, the nonspecific immune response can be compromised in dogs with acute and chronic hyperglycemic conditions. © 2013 Bosco et al.; licensee BioMed Central Ltd
High concentrations of glucose reduce the oxidative metabolism of dog neutrophils <it>in vitro</it>
Abstract Background Dogs are commonly affected by hyperglycemic conditions. Hyperglycemia compromises the immune response and favors bacterial infections; however, reports on the effects of glucose on neutrophil oxidative metabolism and apoptosis are conflicting in humans and rare in dogs. Considering the many complex factors that affect neutrophil oxidative metabolism in vivo, we investigated in vitro the specific effect of high concentrations of glucose on superoxide production and apoptosis rate in neutrophils from healthy dogs. Results The capacity of the neutrophils to reduce tetrazolium nitroblue decreased significantly in the higher concentration of glucose (15.13 ± 9.73% (8 mmol/L) versus 8.93 ± 5.71% (16 mmol/L)). However, there were no changes in tetrazolium nitroblue reduction at different glucose concentrations when the neutrophils were first activated with phorbol myristate acetate. High concentrations of glucose did not affect the viability and apoptosis rate of canine neutrophils either with or without prior camptothecin stimulation. This study provides the first evidence that high concentrations of glucose inhibit the oxidative metabolism of canine neutrophils in vitro in a manner similar to that which occurs in humans, and that the decrease in superoxide production did not increase the apoptosis rate. Conclusions A high concentration of glucose reduces the oxidative metabolism of canine neutrophils in vitro. It is likely that glucose at high concentrations rapidly affects membrane receptors responsible for the activation of NADPH oxidase in neutrophils; therefore, the nonspecific immune response can be compromised in dogs with acute and chronic hyperglycemic conditions.</p
Type 2 diabetes: prevalence and associated factors in a Brazilian community - the BambuĂ health and aging study
Avaliação de percepçÔes sobre gestĂŁo da clĂnica em cursos orientados por competĂȘncia
Efeito da dieta hipoenergĂ©tica sobre a composição corporal e nĂvel sĂ©rico lipĂdico de mulheres adultas com sobrepeso Effect of a low-energy diet on the body composition and serum lipid levels of overweight adult women
OBJETIVO: Verificar o efeito de 12 semanas de dieta hipocalĂłrica sobre a composição corporal e o nĂvel sĂ©rico lipĂdico de mulheres adultas com sobrepeso. MĂTODOS: A amostra foi composta por vinte mulheres (23,80, desvio-padrĂŁo de 2,73 anos) da academia Westfit-Bangu, divididas randomicamente em dois grupos de dez: grupo controle e grupo dieta hipoenergĂ©tica. Foi realizada uma avaliação da composição corporal (massa corporal, percentual de gordura, Ăndice de massa corporal e massa magra) e do nĂvel sĂ©rico lipĂdico (colesterol total, triglicerĂdeos, lipoproteĂna de baixa densidade, lipoproteĂna de alta densidade e lipoproteĂnas de muito baixa densidade). Utilizou-se a estatĂstica descritiva (mĂ©dia e desvio-padrĂŁo) e o teste t de Student na anĂĄlise inter e intragrupos. O nĂvel de significĂąncia foi de p<0,05. RESULTADOS: O grupo dieta hipoenergĂ©tica apresentou reduçÔes significativas (p<0,05) nas variĂĄveis antropomĂ©tricas (massa corporal, percentual de gordura, Ăndice de massa corporal e massa magra) e nas variĂĄveis lipĂdicas (triglicerĂdios, colesterol total, lipoproteĂna de baixa densidade e lipoproteĂnas de muito baixa densidade). Quanto Ă variĂĄvel: lipoproteĂna de alta densidade, foi observada uma redução, porĂ©m nĂŁo significativa. CONCLUSĂO: A partir dos resultados apresentados pode-se concluir que a dieta hipoenergĂ©tica foi uma excelente opção no tratamento da obesidade e no controle do nĂvel sĂ©rico dos lipĂdeos, contribuindo desta forma, para a redução dos riscos cardiovasculares na população estudada.<br>OBJECTIVE: This study verified the effects of a 12-week low-calorie diet on the body composition and serum lipid levels of overweight adult women. METHODS: The sample consisted of 20 females (23.80 years with a standard deviation of 2.73 years) of the Westfit-Bangu gym. They were randomly divided into two groups of ten: the Control Group and the Diet Group. Body composition (body mass, %fat, body mass index and lean body mass) and serum lipid levels (total cholesterol, triglycerides and high-, low- and very low-density lipoproteins) were assessed. Descriptive (central tendency and dispersion) and inferential statistics (Student's t-test) were used for inter and intragroup analysis. The significance level was set at p<0.05. RESULTS: The Diet Group presented a significant decrease (p<0.05) in anthropometric variables (body mass, % of fat, BMI and lean mass) and serum lipids (triglycerides, total cholesterol and low- and very low-density lipoproteins). The level of high-density lipoprotein also decreased but not significantly. CONCLUSION: In conclusion, the low-calorie diet was an excellent option for the treatment of obesity and to control serum lipid levels, decreasing the cardiovascular risk of the studied population