Optical studies of carrier and phonon dynamics in Ga_{1-x}Mn_{x}As

We present a time-resolved optical study of the dynamics of carriers and phonons in Ga_{1-x}Mn_{x}As layers for a series of Mn and hole concentrations. While band filling is the dominant effect in transient optical absorption in low-temperature-grown (LT) GaAs, band gap renormalization effects become important with increasing Mn concentration in Ga_{1-x}Mn_{x}As, as inferred from the sign of the absorption change. We also report direct observation on lattice vibrations in Ga1-xMnxAs layers via reflective electro-optic sampling technique. The data show increasingly fast dephasing of LO phonon oscillations for samples with increasing Mn and hole concentration, which can be understood in term of phonon scattering by the holes.Comment: 13 pages, 3 figures replaced Fig.1 after finding a mistake in previous versio

Global small RNA analysis in fast-growing Arabidopsis thaliana with elevated concentrations of ATP and sugars

BACKGROUND: In higher eukaryotes, small RNAs play a role in regulating gene expression. Overexpression (OE) lines of Arabidopsis thaliana purple acid phosphatase 2 (AtPAP2) were shown to grow faster and exhibit higher ATP and sugar contents. Leaf microarray studies showed that many genes involved in microRNAs (miRNAs) and trans-acting siRNAs (tasiRNAs) biogenesis were significantly changed in the fast-growing lines. In this study, the sRNA profiles of the leaf and the root of 20-day-old plants were sequenced and the impacts of high energy status on sRNA expression were analyzed. RESULTS: 9-13 million reads from each library were mapped to genome. miRNAs, tasiRNAs and natural antisense transcripts-generated small interfering RNAs (natsiRNAs) were identified and compared between libraries. In the leaf of OE lines, 15 known miRNAs increased in abundance and 9 miRNAs decreased in abundance, whereas in the root of OE lines, 2 known miRNAs increased in abundance and 9 miRNAs decreased in abundance. miRNAs with increased abundance in the leaf and root samples of both OE lines (miR158b and miR172a/b) were predicted to target mRNAs coding for Dof zinc finger protein and Apetala 2 (AP2) proteins, respectively. Furthermore, a significant change in the miR173-tasiRNAs-PPR/TPR network was observed in the leaves of both OE lines. CONCLUSION: In this study, the impact of high energy content on the sRNA profiles of Arabidopsis is reported. While the abundance of many stress-induced miRNAs is unaltered, the abundance of some miRNAs related to plant growth and development (miR172 and miR319) is elevated in the fast-growing lines. An induction of miR173-tasiRNAs-PPR/TPR network was also observed in the OE lines. In contrast, only few cis- and trans-natsiRNAs are altered in the fast-growing lines.published_or_final_versio

Growth of anisotropic gold nanoparticles in photoresponsive fluid for UV sensing and erythema prediction

Aim: To develop a novel plasmonic nanosensing technique to monitor the exposure levels of UV light for sunlight disease prevention. Methods: Anisotropic gold nanoparticles were grown inside a UV photoresponsive fluid, which was previously exposed to UV radiation from different sources. The morphology and optical properties of the obtained nanoparticles were monitored by spectroscopy and microscopy. Results: The morphological and optical properties of the nanoparticles were dependent on the UV dose. The UV exposure levels were accurately correlated to the UV minimal doses to produce erythema to different skin types. Conclusion: This plasmonic nanosensing technique can be employed as novel sunlight-indexing tool for monitoring the dangerous level of skin exposure

A BLUEPRINT FOR RESEARCH-LED TEACHING ENGINEERING AT SCHOOLS: A CASE STUDY FOR TAYLOR’S UNIVERSITY

Although it is expected that research conducted at universities and institutions of higher learning will have some positive impact on the teaching quality, the literature seem to point in another direction. Available literature reports zero correlation between teaching and research. However, this need not be the case and a number of recommendations to create a positive correlation between teaching and research are proposed. This paper outlines a framework that utilises the Grand Challenges for Engineering and CDIO to create a clear link between teaching and research in Taylor’s School of Engineering. Aligning the academic staff research objectives to the Grand Challenges, creates a sense of purpose that extends beyond the academic staff to their students. Ensuring that students’ projects and other CDIO activities are derived from the academic staff research interests help creates a learning environment in which research and teaching are integrated. This integration is highly desirable as it benefits both the students and the academic staff

Finite temperature Casimir pistons for electromagnetic field with mixed boundary conditions and its classical limit

In this paper, the finite temperature Casimir force acting on a two-dimensional Casimir piston due to electromagnetic field is computed. It was found that if mixed boundary conditions are assumed on the piston and its opposite wall, then the Casimir force always tends to restore the piston towards the equilibrium position, regardless of the boundary conditions assumed on the walls transverse to the piston. In contrary, if pure boundary conditions are assumed on the piston and the opposite wall, then the Casimir force always tend to pull the piston towards the closer wall and away from the equilibrium position. The nature of the force is not affected by temperature. However, in the high temperature regime, the magnitude of the Casimir force grows linearly with respect to temperature. This shows that the Casimir effect has a classical limit as has been observed in other literatures.Comment: 14 pages, 3 figures, accepted by Journal of Physics

The intracellular domain of sortilin interacts with Amyloid precursor protein and regulates its lysosomal and lipid raft trafficking

The processing of Amyloid precursor protein (APP) is multifaceted, comprising of protein transport, internalization and sequential proteolysis. However, the exact mechanism of APP intracellular trafficking and distribution remains unclear. To determine the interaction between sortilin and APP and the effect of sortilin on APP trafficking and processing, we studied the binding site and its function by mapping experiments, colocalization, coimmunoprecipitation and sucrose gradient fractionation. We identified for the first time that sortilin interacts with APP at both N- and C-terminal regions. The sortilin-FLVHRY (residues 787-792) and APP-NPTYKFFE (residues 759-766) motifs are crucial for the C-terminal interaction. We also found that lack of the FLVHRY motif reduces APP lysosomal targeting and increases APP distribution in lipid rafts in co-transfected HEK293 cells. These results are consistent with our in vivo data where sortilin knockout mice showed a decrease of APP lysosomal distribution and an increase of APP in lipid rafts. We further confirmed that overexpression of sortilin-FLVHRY mutants failed to rescue the lysosomal degradation of APP. Thus, our data suggests that sortilin is implicated in APP lysosomal and lipid raft targeting via its carboxyl-terminal F/YXXXXF/Y motif. Our study provides new molecular insights into APP trafficking and processing.Miao Yang, Balaji Virassamy, Swarna Lekha Vijayaraj, Yoon Lim, Khalil Saadipour, Yan- Jiang Wang, Yan-Chuang Han, Jin-Hua Zhong, Carlos R. Morales, Xin-Fu Zho

Cyclic cosmology from Lagrange-multiplier modified gravity

We investigate cyclic and singularity-free evolutions in a universe governed by Lagrange-multiplier modified gravity, either in scalar-field cosmology, as well as in $f(R)$ one. In the scalar case, cyclicity can be induced by a suitably reconstructed simple potential, and the matter content of the universe can be successfully incorporated. In the case of $f(R)$-gravity, cyclicity can be induced by a suitable reconstructed second function $f_2(R)$ of a very simple form, however the matter evolution cannot be analytically handled. Furthermore, we study the evolution of cosmological perturbations for the two scenarios. For the scalar case the system possesses no wavelike modes due to a dust-like sound speed, while for the $f(R)$ case there exist an oscillation mode of perturbations which indicates a dynamical degree of freedom. Both scenarios allow for stable parameter spaces of cosmological perturbations through the bouncing point.Comment: 8 pages, 3 figures, references added, accepted for publicatio

Hamiltonian Analysis of Lagrange Multiplier Modified Gravity

We develop Hamiltonian formalism for Lagrange Multiplier Modified Gravity. We further calculate the Poisson brackets between constraints and we show that they coincide with the algebra of constraints in Hamiltonian formulation of General Relativity.Comment: 15 pages, references adde