Extrait du catalogue des livres qui se trouvent chez Charles Barrois, libraire, place du Carrousel, n° 26, à Paris.

[Catalogue de libraire. Paris. Barrois, Charles-Jacques. 1808]Avec mode text

Culture-independent study of bacterial communities in tropical river sediment

<p>Ubiquitous microbial communities in river sediments actively govern organic matter decomposition, nutrient recycling, and remediation of toxic compounds. In this study, prokaryotic diversity in two major rivers in central Thailand, the Chao Phraya (CP) and the Tha Chin (TC) distributary was investigated. Significant differences in sediment physicochemical properties, particularly silt content, were noted between the two rivers. Tagged 16S rRNA sequencing on a 454 platform showed that the sediment microbiomes were dominated by <i>Gammaproteobacteria</i> and sulfur/sulfate reducing <i>Deltaproteobacteria</i>, represented by orders <i>Desulfobacteriales</i> and <i>Desulfluromonadales</i> together with organic degraders <i>Betaproteobacteria</i> (orders <i>Burkholderiales</i> and <i>Rhodocyclales</i>) together with the co-existence of <i>Bacteroidetes</i> predominated by <i>Sphingobacteriales</i>. Enrichment of specific bacterial orders was found in the clayey CP and silt-rich TC sediments, including various genera with known metabolic capability on decomposition of organic matter and xenobiotic compounds. The data represent one of the pioneered works revealing heterogeneity of bacteria in river sediments in the tropics.</p> <p>Analysis of bacterial diversity by tagged 16S rRNA sequencing revealed enrichment of specific taxa in CP and TC rivers.</p

Characterization of cellulolytic enzyme system of <i>Schizophyllum commune</i> mutant and evaluation of its efficiency on biomass hydrolysis

<p><i>Schizophyllum commune</i> is a basidiomycete equipped with an efficient cellulolytic enzyme system capable of growth on decaying woods. In this study, production of lignocellulose-degrading enzymes from <i>S. commune</i> mutant G-135 (SC-Cel) on various cellulosic substrates was examined. The highest cellulase activities including CMCase, FPase, and β-glucosidase were obtained on Avicel-PH101 while a wider range of enzymes attacking non-cellulosic polysaccharides and lignin were found when grown on alkaline-pretreated biomass. Proteomic analysis of SC-Cel also revealed a complex enzyme system comprising seven glycosyl hydrolase families with an accessory carbohydrate esterase, polysaccharide lyase, and auxiliary redox enzymes. SC-Cel obtained on Avicel-PH101 effectively hydrolyzed all agricultural residues with the maximum glucan conversion of 98.0% using corn cobs with an enzyme dosage of 5 FPU/g-biomass. The work showed potential of SC-Cel on hydrolysis of various herbaceous biomass with enhanced efficiency by addition external β-xylosidase.</p> <p>Enzymatic hydrolysis of alkaline-pretreated biomass by Sc-Cel and yield enhancement by external β-xylosidase (BX).</p

Additional file 1: of Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring

Effect of pH on EndoPG activity. Effect of temperature on EndoPG activity. Effect of pH on stability of EndoPG activity. Effect of temperature (20–60 °C) on stability of EndoPG activity. RAW data of enzyme activities were calculated and showed as volumetric activity (U/ml) and % relative activity. Standard deviations (SD) were calculated from volumetric activity. (XLS 40 kb

Comparative distribution of fungal diversity in sediment and water.

<p>(A) Principal component analysis (PCA) based on percent relative abundance of fungal taxa at genus level in sediment (orange) and water (blue). (B) Bar chart with extended error bar for pair-wise comparisons of fungal genera between sediment (orange) and water (blue) samples.</p

Map of sample collection sites.

<p>The sampling areas are in Bangkok, Pathumthani, Nonthaburi, and Nakornpathom provinces.</p

Statistical analysis and biodiversity index of bacterial 16S rRNA gene and fungal ITS1 tagged-pyrosequences at the 97% similarity.

<p>Statistical analysis and biodiversity index of bacterial 16S rRNA gene and fungal ITS1 tagged-pyrosequences at the 97% similarity.</p

Comparison of fungal diversity in sediment and water.

<p>Percent relative abundance of fungal phyla based on ITS sequence abundance are shown. Left and right bars represent relative abundance collected from sediment and water, respectively.</p

Comparative distribution of bacterial diversity in sediment and water.

<p>(A) Principal component analysis (PCA) based on percent relative abundance of bacterial taxa at genus level in sediment (orange) and water (blue). (B) Bar chart with extended error bar for pair-wise comparisons of bacterial genera between sediment (orange) and water (blue) samples.</p

Summary of pyrosequencing dataset.

<p>Summary of pyrosequencing dataset.</p