Proteomic Analysis of the Effect of DHA-Phospholipids from Large Yellow Croaker Roe on Hyperlipidemic Mice

Previously, we found that phospholipids derived from large yellow croaker (<i>Pseudosciaena crocea</i>) roe had a higher level of docosahexaenoic acid (DHA–PL), which had beneficial effects on lipid metabolism. However, the mechanism by which DHA–PL from <i>P. crocea</i> roe exerts these effects has not yet been illuminated. Herein, we investigated the underlying molecular action of DHA–PL by examining changes in liver protein expression in control, hyperlipidemic, and DHA–PL-treated mice. A total of 16 proteins, 9 up-regulated and 7 down-regulated, were identified and classified into several metabolic pathways, such as fat digestion and absorption, peroxisome proliferator activated receptor (PPAR) signaling, and antigen processing and presentation; the largest functional class found was that of fat digestion and absorption. We revealed Apoa1 to be a biomarker of DHA–PL effects on hyperlipidemic mice by DHA–PL diet. These results not only improve our current understanding of hyperlipidemic regulation by DHA–PL, but also suggest that DHA–PL should be applied as a beneficial food additive